Abstract
Superficial zone protein (SZP) is a key mediator of boundary lubrication of articular cartilage in joints. In this investigation, we made the unexpected discovery that SZP was expressed in infrapatellar fat pad (IFP) from bovine knee. Quantitative analysis of secreted proteins in the medium of the IFP stromal cells demonstrated a significant stimulation by TGF-β1 and BMP-7. Real-time PCR analysis revealed the SZP expression was up-regulated by TGF-β1 and BMP-7. Chondrogenically differentiated IFP progenitor cells were stimulated by TGF-β1 and BMP-7 to synthesize and secrete SZP. SZP mRNA was significantly up-regulated by chondrogenic induction for 21 days. These findings indicate that the stimulation of SZP expression by TGF-β and BMP-7 may lead to functional improvement of damaged intraarticular tissues and that IFP progenitor cells may be a potential useful source for inducing superficial zone of articular cartilage by tissue engineering for regeneration of damaged articular cartilage due to osteoarthritis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 148-153 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 376 |
| Issue number | 1 |
| DOIs | |
| State | Published - Nov 7 2008 |
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Keywords
- BMP-7
- Chondrogenesis
- Infrapatellar fat pad
- Lubrication
- Lubricin
- Stem/progenitor cells
- Superficial zone protein
- TGF-β
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Induction of chondrogenesis and expression of superficial zone protein (SZP)/lubricin by mesenchymal progenitors in the infrapatellar fat pad of the knee joint treated with TGF-β1 and BMP-7. / Lee, Sang Yang; Nakagawa, Toshiyuki; Reddi, A Hari.
In: Biochemical and Biophysical Research Communications, Vol. 376, No. 1, 07.11.2008, p. 148-153.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of chondrogenesis and expression of superficial zone protein (SZP)/lubricin by mesenchymal progenitors in the infrapatellar fat pad of the knee joint treated with TGF-β1 and BMP-7
AU - Lee, Sang Yang
AU - Nakagawa, Toshiyuki
AU - Reddi, A Hari
PY - 2008/11/7
Y1 - 2008/11/7
N2 - Superficial zone protein (SZP) is a key mediator of boundary lubrication of articular cartilage in joints. In this investigation, we made the unexpected discovery that SZP was expressed in infrapatellar fat pad (IFP) from bovine knee. Quantitative analysis of secreted proteins in the medium of the IFP stromal cells demonstrated a significant stimulation by TGF-β1 and BMP-7. Real-time PCR analysis revealed the SZP expression was up-regulated by TGF-β1 and BMP-7. Chondrogenically differentiated IFP progenitor cells were stimulated by TGF-β1 and BMP-7 to synthesize and secrete SZP. SZP mRNA was significantly up-regulated by chondrogenic induction for 21 days. These findings indicate that the stimulation of SZP expression by TGF-β and BMP-7 may lead to functional improvement of damaged intraarticular tissues and that IFP progenitor cells may be a potential useful source for inducing superficial zone of articular cartilage by tissue engineering for regeneration of damaged articular cartilage due to osteoarthritis.
AB - Superficial zone protein (SZP) is a key mediator of boundary lubrication of articular cartilage in joints. In this investigation, we made the unexpected discovery that SZP was expressed in infrapatellar fat pad (IFP) from bovine knee. Quantitative analysis of secreted proteins in the medium of the IFP stromal cells demonstrated a significant stimulation by TGF-β1 and BMP-7. Real-time PCR analysis revealed the SZP expression was up-regulated by TGF-β1 and BMP-7. Chondrogenically differentiated IFP progenitor cells were stimulated by TGF-β1 and BMP-7 to synthesize and secrete SZP. SZP mRNA was significantly up-regulated by chondrogenic induction for 21 days. These findings indicate that the stimulation of SZP expression by TGF-β and BMP-7 may lead to functional improvement of damaged intraarticular tissues and that IFP progenitor cells may be a potential useful source for inducing superficial zone of articular cartilage by tissue engineering for regeneration of damaged articular cartilage due to osteoarthritis.
KW - BMP-7
KW - Chondrogenesis
KW - Infrapatellar fat pad
KW - Lubrication
KW - Lubricin
KW - Stem/progenitor cells
KW - Superficial zone protein
KW - TGF-β
UR - http://www.scopus.com/inward/record.url?scp=52049101630&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=52049101630&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2008.08.138
DO - 10.1016/j.bbrc.2008.08.138
M3 - Article
C2 - 18774772
AN - SCOPUS:52049101630
VL - 376
SP - 148
EP - 153
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -