Induction of chondrogenesis and expression of superficial zone protein (SZP)/lubricin by mesenchymal progenitors in the infrapatellar fat pad of the knee joint treated with TGF-β1 and BMP-7

Sang Yang Lee, Toshiyuki Nakagawa, A Hari Reddi

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Superficial zone protein (SZP) is a key mediator of boundary lubrication of articular cartilage in joints. In this investigation, we made the unexpected discovery that SZP was expressed in infrapatellar fat pad (IFP) from bovine knee. Quantitative analysis of secreted proteins in the medium of the IFP stromal cells demonstrated a significant stimulation by TGF-β1 and BMP-7. Real-time PCR analysis revealed the SZP expression was up-regulated by TGF-β1 and BMP-7. Chondrogenically differentiated IFP progenitor cells were stimulated by TGF-β1 and BMP-7 to synthesize and secrete SZP. SZP mRNA was significantly up-regulated by chondrogenic induction for 21 days. These findings indicate that the stimulation of SZP expression by TGF-β and BMP-7 may lead to functional improvement of damaged intraarticular tissues and that IFP progenitor cells may be a potential useful source for inducing superficial zone of articular cartilage by tissue engineering for regeneration of damaged articular cartilage due to osteoarthritis.

Original languageEnglish (US)
Pages (from-to)148-153
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume376
Issue number1
DOIs
StatePublished - Nov 7 2008

Fingerprint

Bone Morphogenetic Protein 7
Chondrogenesis
Knee Joint
Adipose Tissue
Fats
Cartilage
Articular Cartilage
Proteins
Stem Cells
Lubrication
Tissue Engineering
Stromal Cells
lubricin
Tissue engineering
Osteoarthritis
Real-Time Polymerase Chain Reaction
Regeneration
Knee
Joints
Tissue

Keywords

  • BMP-7
  • Chondrogenesis
  • Infrapatellar fat pad
  • Lubrication
  • Lubricin
  • Stem/progenitor cells
  • Superficial zone protein
  • TGF-β

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

@article{2e57e25d586649d8881f5fe842f62a00,
title = "Induction of chondrogenesis and expression of superficial zone protein (SZP)/lubricin by mesenchymal progenitors in the infrapatellar fat pad of the knee joint treated with TGF-β1 and BMP-7",
abstract = "Superficial zone protein (SZP) is a key mediator of boundary lubrication of articular cartilage in joints. In this investigation, we made the unexpected discovery that SZP was expressed in infrapatellar fat pad (IFP) from bovine knee. Quantitative analysis of secreted proteins in the medium of the IFP stromal cells demonstrated a significant stimulation by TGF-β1 and BMP-7. Real-time PCR analysis revealed the SZP expression was up-regulated by TGF-β1 and BMP-7. Chondrogenically differentiated IFP progenitor cells were stimulated by TGF-β1 and BMP-7 to synthesize and secrete SZP. SZP mRNA was significantly up-regulated by chondrogenic induction for 21 days. These findings indicate that the stimulation of SZP expression by TGF-β and BMP-7 may lead to functional improvement of damaged intraarticular tissues and that IFP progenitor cells may be a potential useful source for inducing superficial zone of articular cartilage by tissue engineering for regeneration of damaged articular cartilage due to osteoarthritis.",
keywords = "BMP-7, Chondrogenesis, Infrapatellar fat pad, Lubrication, Lubricin, Stem/progenitor cells, Superficial zone protein, TGF-β",
author = "Lee, {Sang Yang} and Toshiyuki Nakagawa and Reddi, {A Hari}",
year = "2008",
month = "11",
day = "7",
doi = "10.1016/j.bbrc.2008.08.138",
language = "English (US)",
volume = "376",
pages = "148--153",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Induction of chondrogenesis and expression of superficial zone protein (SZP)/lubricin by mesenchymal progenitors in the infrapatellar fat pad of the knee joint treated with TGF-β1 and BMP-7

AU - Lee, Sang Yang

AU - Nakagawa, Toshiyuki

AU - Reddi, A Hari

PY - 2008/11/7

Y1 - 2008/11/7

N2 - Superficial zone protein (SZP) is a key mediator of boundary lubrication of articular cartilage in joints. In this investigation, we made the unexpected discovery that SZP was expressed in infrapatellar fat pad (IFP) from bovine knee. Quantitative analysis of secreted proteins in the medium of the IFP stromal cells demonstrated a significant stimulation by TGF-β1 and BMP-7. Real-time PCR analysis revealed the SZP expression was up-regulated by TGF-β1 and BMP-7. Chondrogenically differentiated IFP progenitor cells were stimulated by TGF-β1 and BMP-7 to synthesize and secrete SZP. SZP mRNA was significantly up-regulated by chondrogenic induction for 21 days. These findings indicate that the stimulation of SZP expression by TGF-β and BMP-7 may lead to functional improvement of damaged intraarticular tissues and that IFP progenitor cells may be a potential useful source for inducing superficial zone of articular cartilage by tissue engineering for regeneration of damaged articular cartilage due to osteoarthritis.

AB - Superficial zone protein (SZP) is a key mediator of boundary lubrication of articular cartilage in joints. In this investigation, we made the unexpected discovery that SZP was expressed in infrapatellar fat pad (IFP) from bovine knee. Quantitative analysis of secreted proteins in the medium of the IFP stromal cells demonstrated a significant stimulation by TGF-β1 and BMP-7. Real-time PCR analysis revealed the SZP expression was up-regulated by TGF-β1 and BMP-7. Chondrogenically differentiated IFP progenitor cells were stimulated by TGF-β1 and BMP-7 to synthesize and secrete SZP. SZP mRNA was significantly up-regulated by chondrogenic induction for 21 days. These findings indicate that the stimulation of SZP expression by TGF-β and BMP-7 may lead to functional improvement of damaged intraarticular tissues and that IFP progenitor cells may be a potential useful source for inducing superficial zone of articular cartilage by tissue engineering for regeneration of damaged articular cartilage due to osteoarthritis.

KW - BMP-7

KW - Chondrogenesis

KW - Infrapatellar fat pad

KW - Lubrication

KW - Lubricin

KW - Stem/progenitor cells

KW - Superficial zone protein

KW - TGF-β

UR - http://www.scopus.com/inward/record.url?scp=52049101630&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=52049101630&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2008.08.138

DO - 10.1016/j.bbrc.2008.08.138

M3 - Article

C2 - 18774772

AN - SCOPUS:52049101630

VL - 376

SP - 148

EP - 153

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -