Induction of 70-kDa heat shock protein and hsp70 mRNA following transient focal cerebral ischemia in the rat

Hiroyuki Kinouchi, Frank R Sharp, Mary P. Hill, Jari Koistinaho, Stephen M. Sagar, Pak H. Chan

Research output: Contribution to journalArticle

167 Scopus citations

Abstract

Induction of the 70-kDa heat shock protein (HSP70) was demonstrated immunocytochemically in adult rats 4 h to 7 days following temporary middle cerebra) artery (MCA) occlusions lasting 30, 60, or 90 min. Maximal HSP70 induction occurred ∼24 h following ischemia. Thirty minutes of ischemia induced HSP70 in neurons throughout the cortex in the MCA distribution, whereas 90 min of ischemia induced HSP70 in neurons in the penumbra. HSP70 protein was induced in endothelial cells in infarcted neocortex following 60-90 min of MCA occlusion, and HSP70 was induced in endothelial cells in infarcted regions of lateral striatum following 30-90 min of MCA occlusion. hsp70 mRNA was induced in the MCA distribution in cortex and to a lesser extent in striatum at 2 h to 3 days following 60 min of ischemia. It is proposed that brief ischemia induces hsp70 mRNA and HSP70 protein in the cells most vulnerable to ischemia -the neurons. HSP70 protein is not induced in most neurons and glia following 60-90 min of ischemia in areas destined to infarct, whereas it is induced in vascular endothelial cells.

Original languageEnglish (US)
Pages (from-to)105-115
Number of pages11
JournalJournal of Cerebral Blood Flow and Metabolism
Volume13
Issue number1
StatePublished - 1993
Externally publishedYes

Keywords

  • Focal brain
  • Hsp70 mRNA
  • Middle cerebral artery
  • Stress and heat shock protein
  • Stroke

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Neuroscience(all)

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