Induction in the expression of an unusual proline-rich protein by pig tracheal surface epithelial cells maintained in primary culture

Johannes Tesfaigzi, Gang An, Reen Wu, Don M. Carlson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Primary cell culture is a valuable tool for studying the regulation of gene expression since many differentiated traits are conserved. Cells cultured from the epithelial lining of pig trachea were selected as a model system for mucin synthesis. RNAs were isolated from pig trachea epithelial linings and from pig trachea surface epithelial cells cultured in serum-free media. Cell free translations showed an unusually high incorporation of [3H]proline into a relatively small protein (about 20 kDa), but only with RNA from the cells in culture. RNA prepared from pig trachea cells immediately before placing the cells in culture (day 0) did not contain mRNA encoding this unusual proline-rich protein. However, the expression of this protein was dramatically induced within 2 days of maintaining the cells in culture.

Original languageEnglish (US)
Pages (from-to)1304-1309
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume172
Issue number3
DOIs
StatePublished - Nov 15 1990

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Trachea
Cell culture
Proline
Swine
Epithelial Cells
RNA
Linings
Cell Culture Techniques
Proteins
Serum-Free Culture Media
Mucins
Gene expression
Primary Cell Culture
Gene Expression Regulation
Cultured Cells
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Induction in the expression of an unusual proline-rich protein by pig tracheal surface epithelial cells maintained in primary culture. / Tesfaigzi, Johannes; An, Gang; Wu, Reen; Carlson, Don M.

In: Biochemical and Biophysical Research Communications, Vol. 172, No. 3, 15.11.1990, p. 1304-1309.

Research output: Contribution to journalArticle

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