Abstract
Primary cell culture is a valuable tool for studying the regulation of gene expression since many differentiated traits are conserved. Cells cultured from the epithelial lining of pig trachea were selected as a model system for mucin synthesis. RNAs were isolated from pig trachea epithelial linings and from pig trachea surface epithelial cells cultured in serum-free media. Cell free translations showed an unusually high incorporation of [3H]proline into a relatively small protein (about 20 kDa), but only with RNA from the cells in culture. RNA prepared from pig trachea cells immediately before placing the cells in culture (day 0) did not contain mRNA encoding this unusual proline-rich protein. However, the expression of this protein was dramatically induced within 2 days of maintaining the cells in culture.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1304-1309 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 172 |
| Issue number | 3 |
| DOIs | |
| State | Published - Nov 15 1990 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Induction in the expression of an unusual proline-rich protein by pig tracheal surface epithelial cells maintained in primary culture. / Tesfaigzi, Johannes; An, Gang; Wu, Reen; Carlson, Don M.
In: Biochemical and Biophysical Research Communications, Vol. 172, No. 3, 15.11.1990, p. 1304-1309.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction in the expression of an unusual proline-rich protein by pig tracheal surface epithelial cells maintained in primary culture
AU - Tesfaigzi, Johannes
AU - An, Gang
AU - Wu, Reen
AU - Carlson, Don M.
PY - 1990/11/15
Y1 - 1990/11/15
N2 - Primary cell culture is a valuable tool for studying the regulation of gene expression since many differentiated traits are conserved. Cells cultured from the epithelial lining of pig trachea were selected as a model system for mucin synthesis. RNAs were isolated from pig trachea epithelial linings and from pig trachea surface epithelial cells cultured in serum-free media. Cell free translations showed an unusually high incorporation of [3H]proline into a relatively small protein (about 20 kDa), but only with RNA from the cells in culture. RNA prepared from pig trachea cells immediately before placing the cells in culture (day 0) did not contain mRNA encoding this unusual proline-rich protein. However, the expression of this protein was dramatically induced within 2 days of maintaining the cells in culture.
AB - Primary cell culture is a valuable tool for studying the regulation of gene expression since many differentiated traits are conserved. Cells cultured from the epithelial lining of pig trachea were selected as a model system for mucin synthesis. RNAs were isolated from pig trachea epithelial linings and from pig trachea surface epithelial cells cultured in serum-free media. Cell free translations showed an unusually high incorporation of [3H]proline into a relatively small protein (about 20 kDa), but only with RNA from the cells in culture. RNA prepared from pig trachea cells immediately before placing the cells in culture (day 0) did not contain mRNA encoding this unusual proline-rich protein. However, the expression of this protein was dramatically induced within 2 days of maintaining the cells in culture.
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U2 - 10.1016/0006-291X(90)91591-F
DO - 10.1016/0006-291X(90)91591-F
M3 - Article
C2 - 1700906
AN - SCOPUS:0025252435
VL - 172
SP - 1304
EP - 1309
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -