Induction and assessment of hypoxia in glioblastoma cells in vitro

Jean Pierre Gagner, Mirna Lechpammer, David Zagzag

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

To simulate and study the hypoxic microenvironment associated with intracerebral glioma in vivo, simple and reproducible methods are described and discussed for inducing hypoxia or chemical pseudohypoxia in glioma cell cultures and assessing their effects on the expression and nuclear translocation of hypoxia-inducible factor (HIF)-1α, a key transcriptional factor of oxygen homeostasis, by Western blot analysis and immunocytochemistry.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages111-123
Number of pages13
Volume1741
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1741
ISSN (Print)1064-3745

Fingerprint

Glioblastoma
Glioma
Hypoxia-Inducible Factor 1
Homeostasis
Cell Culture Techniques
Western Blotting
Immunohistochemistry
Oxygen
In Vitro Techniques
Hypoxia

Keywords

  • Chemical pseudohypoxia
  • Glioblastoma
  • Glioma cell lines
  • Hypoxia cell culture chamber
  • Hypoxia-inducible factor-1α
  • Hypoxia-regulated genes
  • Immunocytochemistry
  • Prolyl hydroxylases
  • Tumor hypoxia
  • Western blot analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Gagner, J. P., Lechpammer, M., & Zagzag, D. (2018). Induction and assessment of hypoxia in glioblastoma cells in vitro. In Methods in Molecular Biology (Vol. 1741, pp. 111-123). (Methods in Molecular Biology; Vol. 1741). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7659-1_9

Induction and assessment of hypoxia in glioblastoma cells in vitro. / Gagner, Jean Pierre; Lechpammer, Mirna; Zagzag, David.

Methods in Molecular Biology. Vol. 1741 Humana Press Inc., 2018. p. 111-123 (Methods in Molecular Biology; Vol. 1741).

Research output: Chapter in Book/Report/Conference proceedingChapter

Gagner, JP, Lechpammer, M & Zagzag, D 2018, Induction and assessment of hypoxia in glioblastoma cells in vitro. in Methods in Molecular Biology. vol. 1741, Methods in Molecular Biology, vol. 1741, Humana Press Inc., pp. 111-123. https://doi.org/10.1007/978-1-4939-7659-1_9
Gagner JP, Lechpammer M, Zagzag D. Induction and assessment of hypoxia in glioblastoma cells in vitro. In Methods in Molecular Biology. Vol. 1741. Humana Press Inc. 2018. p. 111-123. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7659-1_9
Gagner, Jean Pierre ; Lechpammer, Mirna ; Zagzag, David. / Induction and assessment of hypoxia in glioblastoma cells in vitro. Methods in Molecular Biology. Vol. 1741 Humana Press Inc., 2018. pp. 111-123 (Methods in Molecular Biology).
@inbook{1b8c9da4aa44456b8f24320b9c24e9d0,
title = "Induction and assessment of hypoxia in glioblastoma cells in vitro",
abstract = "To simulate and study the hypoxic microenvironment associated with intracerebral glioma in vivo, simple and reproducible methods are described and discussed for inducing hypoxia or chemical pseudohypoxia in glioma cell cultures and assessing their effects on the expression and nuclear translocation of hypoxia-inducible factor (HIF)-1α, a key transcriptional factor of oxygen homeostasis, by Western blot analysis and immunocytochemistry.",
keywords = "Chemical pseudohypoxia, Glioblastoma, Glioma cell lines, Hypoxia cell culture chamber, Hypoxia-inducible factor-1α, Hypoxia-regulated genes, Immunocytochemistry, Prolyl hydroxylases, Tumor hypoxia, Western blot analysis",
author = "Gagner, {Jean Pierre} and Mirna Lechpammer and David Zagzag",
year = "2018",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-7659-1_9",
language = "English (US)",
volume = "1741",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "111--123",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Induction and assessment of hypoxia in glioblastoma cells in vitro

AU - Gagner, Jean Pierre

AU - Lechpammer, Mirna

AU - Zagzag, David

PY - 2018/1/1

Y1 - 2018/1/1

N2 - To simulate and study the hypoxic microenvironment associated with intracerebral glioma in vivo, simple and reproducible methods are described and discussed for inducing hypoxia or chemical pseudohypoxia in glioma cell cultures and assessing their effects on the expression and nuclear translocation of hypoxia-inducible factor (HIF)-1α, a key transcriptional factor of oxygen homeostasis, by Western blot analysis and immunocytochemistry.

AB - To simulate and study the hypoxic microenvironment associated with intracerebral glioma in vivo, simple and reproducible methods are described and discussed for inducing hypoxia or chemical pseudohypoxia in glioma cell cultures and assessing their effects on the expression and nuclear translocation of hypoxia-inducible factor (HIF)-1α, a key transcriptional factor of oxygen homeostasis, by Western blot analysis and immunocytochemistry.

KW - Chemical pseudohypoxia

KW - Glioblastoma

KW - Glioma cell lines

KW - Hypoxia cell culture chamber

KW - Hypoxia-inducible factor-1α

KW - Hypoxia-regulated genes

KW - Immunocytochemistry

KW - Prolyl hydroxylases

KW - Tumor hypoxia

KW - Western blot analysis

UR - http://www.scopus.com/inward/record.url?scp=85041743950&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041743950&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-7659-1_9

DO - 10.1007/978-1-4939-7659-1_9

M3 - Chapter

C2 - 29392695

AN - SCOPUS:85041743950

VL - 1741

T3 - Methods in Molecular Biology

SP - 111

EP - 123

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -