Induction and assessment of hypoxia in glioblastoma cells in vitro

Jean Pierre Gagner, Mirna Lechpammer, David Zagzag

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

To simulate and study the hypoxic microenvironment associated with intracerebral glioma in vivo, simple and reproducible methods are described and discussed for inducing hypoxia or chemical pseudohypoxia in glioma cell cultures and assessing their effects on the expression and nuclear translocation of hypoxia-inducible factor (HIF)-1α, a key transcriptional factor of oxygen homeostasis, by Western blot analysis and immunocytochemistry.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages111-123
Number of pages13
Volume1741
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1741
ISSN (Print)1064-3745

Keywords

  • Chemical pseudohypoxia
  • Glioblastoma
  • Glioma cell lines
  • Hypoxia cell culture chamber
  • Hypoxia-inducible factor-1α
  • Hypoxia-regulated genes
  • Immunocytochemistry
  • Prolyl hydroxylases
  • Tumor hypoxia
  • Western blot analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Gagner, J. P., Lechpammer, M., & Zagzag, D. (2018). Induction and assessment of hypoxia in glioblastoma cells in vitro. In Methods in Molecular Biology (Vol. 1741, pp. 111-123). (Methods in Molecular Biology; Vol. 1741). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7659-1_9