Induced pluripotent stem cells for neural tissue engineering

Aijun Wang, Zhenyu Tang, In Hyun Park, Yiqian Zhu, Shyam Patel, George Q. Daley, Song Li

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Induced pluripotent stem cells (iPSCs) hold great promise for cell therapies and tissue engineering. Neural crest stem cells (NCSCs) are multipotent and represent a valuable system to investigate iPSC differentiation and therapeutic potential. Here we derived NCSCs from human iPSCs and embryonic stem cells (ESCs), and investigated the potential of NCSCs for neural tissue engineering. The differentiation of iPSCs and the expansion of derived NCSCs varied in different cell lines, but all NCSC lines were capable of differentiating into mesodermal and ectodermal lineages, including neural cells. Tissue-engineered nerve conduits were fabricated by seeding NCSCs into nanofibrous tubular scaffolds, and used as a bridge for transected sciatic nerves in a rat model. Electrophysiological analysis showed that only NCSC-engrafted nerve conduits resulted in an accelerated regeneration of sciatic nerves at 1 month. Histological analysis demonstrated that NCSC transplantation promoted axonal myelination. Furthermore, NCSCs differentiated into Schwann cells and were integrated into the myelin sheath around axons. No teratoma formation was observed for up to 1 year after NCSC transplantation in vivo. This study demonstrates that iPSC-derived multipotent NCSCs can be directly used for tissue engineering and that the approach that combines stem cells and scaffolds has tremendous potential for regenerative medicine applications.

Original languageEnglish (US)
Pages (from-to)5023-5032
Number of pages10
JournalBiomaterials
Volume32
Issue number22
DOIs
StatePublished - Aug 2011

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Induced Pluripotent Stem Cells
Neural Stem Cells
Neural Crest
Tissue Engineering
Stem cells
Tissue engineering
Stem Cell Transplantation
Sciatic Nerve
Multipotent Stem Cells
Cell Engineering
Nerve Tissue
Cell Line
Regenerative Medicine
Scaffolds
Schwann Cells
Teratoma
Embryonic Stem Cells
Myelin Sheath
Cell- and Tissue-Based Therapy
Cells

Keywords

  • Nanofibers
  • Nerve guide
  • Nerve tissue engineering
  • Neural crest stem cell
  • Scaffold
  • Stem cells

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Wang, A., Tang, Z., Park, I. H., Zhu, Y., Patel, S., Daley, G. Q., & Li, S. (2011). Induced pluripotent stem cells for neural tissue engineering. Biomaterials, 32(22), 5023-5032. https://doi.org/10.1016/j.biomaterials.2011.03.070

Induced pluripotent stem cells for neural tissue engineering. / Wang, Aijun; Tang, Zhenyu; Park, In Hyun; Zhu, Yiqian; Patel, Shyam; Daley, George Q.; Li, Song.

In: Biomaterials, Vol. 32, No. 22, 08.2011, p. 5023-5032.

Research output: Contribution to journalArticle

Wang, A, Tang, Z, Park, IH, Zhu, Y, Patel, S, Daley, GQ & Li, S 2011, 'Induced pluripotent stem cells for neural tissue engineering', Biomaterials, vol. 32, no. 22, pp. 5023-5032. https://doi.org/10.1016/j.biomaterials.2011.03.070
Wang, Aijun ; Tang, Zhenyu ; Park, In Hyun ; Zhu, Yiqian ; Patel, Shyam ; Daley, George Q. ; Li, Song. / Induced pluripotent stem cells for neural tissue engineering. In: Biomaterials. 2011 ; Vol. 32, No. 22. pp. 5023-5032.
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