Abstract
The [14C]-deoxyglucose (2DG) technique has been used to map the effects of increasing intensities of wide band noise on 2DG uptake in mongolian gerbil brain auditory structures. Animals were injected with [14C]2DG and exposed to silence or continuous wide band noise at 25 dB, 45 dB, 65 dB, 85 dB or 105 dB SPL. Brains were removed, frozen-sectioned, and autoradiographed on X-ray film. The ratio of the optical density of gray matter structures to the optical density of cerebellar peduncles in each animal was used to semiquantitate the results. The dorsal and ventral cochlear nuclei, superior olive/trapezoid body, inferior colliculus, and the dorsal and ventral nuclei of the lateral lemniscus all showed increases in 2DG uptake during exposure to wide band noise (WBN). As noise intensity increased from 0 to 105 dB SPL, 2DG uptake increased regularly to a maximum at 85 or 105 dB SPL. As WBN intensity increased, deeper layers of inferior colliculus were activated. The medial geniculate nucleus and auditory cortex xhowed a lesser increase in 2DG uptake during noise exposure. Non-auditory structures, including the cerebellar cortex and the medullary reticular nuclei, showed no increase in 2DG uptake during noise exposure at any intensity tested.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 87-96 |
| Number of pages | 10 |
| Journal | Brain Research |
| Volume | 230 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Dec 28 1981 |
| Externally published | Yes |
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Keywords
- 2-deosyglucose
- audition
- CNS metabolism
- noise exposure
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
Increasing intensities of wide band noise increase [14C]2-deoxyglucose uptake in gerbil central auditory structures. / Sharp, Frank R; Ryan, Allen F.; Goodwin, Paul; Woolf, Nigel K.
In: Brain Research, Vol. 230, No. 1-2, 28.12.1981, p. 87-96.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Increasing intensities of wide band noise increase [14C]2-deoxyglucose uptake in gerbil central auditory structures
AU - Sharp, Frank R
AU - Ryan, Allen F.
AU - Goodwin, Paul
AU - Woolf, Nigel K.
PY - 1981/12/28
Y1 - 1981/12/28
N2 - The [14C]-deoxyglucose (2DG) technique has been used to map the effects of increasing intensities of wide band noise on 2DG uptake in mongolian gerbil brain auditory structures. Animals were injected with [14C]2DG and exposed to silence or continuous wide band noise at 25 dB, 45 dB, 65 dB, 85 dB or 105 dB SPL. Brains were removed, frozen-sectioned, and autoradiographed on X-ray film. The ratio of the optical density of gray matter structures to the optical density of cerebellar peduncles in each animal was used to semiquantitate the results. The dorsal and ventral cochlear nuclei, superior olive/trapezoid body, inferior colliculus, and the dorsal and ventral nuclei of the lateral lemniscus all showed increases in 2DG uptake during exposure to wide band noise (WBN). As noise intensity increased from 0 to 105 dB SPL, 2DG uptake increased regularly to a maximum at 85 or 105 dB SPL. As WBN intensity increased, deeper layers of inferior colliculus were activated. The medial geniculate nucleus and auditory cortex xhowed a lesser increase in 2DG uptake during noise exposure. Non-auditory structures, including the cerebellar cortex and the medullary reticular nuclei, showed no increase in 2DG uptake during noise exposure at any intensity tested.
AB - The [14C]-deoxyglucose (2DG) technique has been used to map the effects of increasing intensities of wide band noise on 2DG uptake in mongolian gerbil brain auditory structures. Animals were injected with [14C]2DG and exposed to silence or continuous wide band noise at 25 dB, 45 dB, 65 dB, 85 dB or 105 dB SPL. Brains were removed, frozen-sectioned, and autoradiographed on X-ray film. The ratio of the optical density of gray matter structures to the optical density of cerebellar peduncles in each animal was used to semiquantitate the results. The dorsal and ventral cochlear nuclei, superior olive/trapezoid body, inferior colliculus, and the dorsal and ventral nuclei of the lateral lemniscus all showed increases in 2DG uptake during exposure to wide band noise (WBN). As noise intensity increased from 0 to 105 dB SPL, 2DG uptake increased regularly to a maximum at 85 or 105 dB SPL. As WBN intensity increased, deeper layers of inferior colliculus were activated. The medial geniculate nucleus and auditory cortex xhowed a lesser increase in 2DG uptake during noise exposure. Non-auditory structures, including the cerebellar cortex and the medullary reticular nuclei, showed no increase in 2DG uptake during noise exposure at any intensity tested.
KW - 2-deosyglucose
KW - audition
KW - CNS metabolism
KW - noise exposure
UR - http://www.scopus.com/inward/record.url?scp=0019823297&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019823297&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(81)90393-0
DO - 10.1016/0006-8993(81)90393-0
M3 - Article
C2 - 7317792
AN - SCOPUS:0019823297
VL - 230
SP - 87
EP - 96
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -