Increased vulnerability of the midly traumatized rat brain to cerebral ischemia: the use of controlled secondary ischemia as a research tool to identify common or different mechanisms contributing to mechanical and ischemic brain injury

L. W. Jenkins, K. Moszynski, Bruce G Lyeth, W. Lewelt, D. S. DeWitt, A. Allen, C. E. Dixon, J. T. Povlishock, T. J. Majewski, G. L. Clifton, H. F. Young, D. P. Becker, R. L. Hayes

Research output: Contribution to journalArticle

223 Scopus citations

Abstract

Fasted Wistar rats were subjected to either a mild mechanical injury, 6 min of transient forebrain ischemia, or a mild mechanical injury followed 1 h later by 6 min of forebrain ischemia. EEG and evoked potentials were assessed intermittently and morphological analyses were performed after 7 das postinjury survival. In all groups complete qualitative recovery of electrical activity and general behavior was observed with 7-day survival. However, rats subjected to combined concussion and ischemia displayed EEG spike activity and a delayed return of EEG and evoked potentials during acute recovery not evident in other groups. No overt neuronal cells loss was seen in trauma alone and was minimal or absent in ischemia alone. However, extensive bilateral CA1 and subicular pyramidal cell loss was found in the septal and mid-dorsal hippocampi in the combined trauma and ischemia group. In contrast, no overt axonal injury was found in any group. We conclude that even mild mechanical injury can potentiate selective ischemic hippocampal neuronal necrosis in the absence of overt axonal injury. This potentiation also occurs in conjunction with more generalized electrophysiological disturbances such as EEG evidence of postischemic neuronal hyperactivity suggesting that mild concussion may also decrease the threshold for post-ischemic neuronal excitation. These results suggest the potential of this model for examining common or different injury mechanisms in mechanical and ischemic brain injury.

Original languageEnglish (US)
Pages (from-to)211-224
Number of pages14
JournalBrain Research
Volume477
Issue number1-2
DOIs
StatePublished - Jan 16 1989
Externally publishedYes

Keywords

  • Axonal injury
  • Concussion
  • Delayed neuronal necrosis
  • Hippocampal hyperactivity
  • Secondary ischemia
  • Selective vulnerability

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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  • Cite this

    Jenkins, L. W., Moszynski, K., Lyeth, B. G., Lewelt, W., DeWitt, D. S., Allen, A., Dixon, C. E., Povlishock, J. T., Majewski, T. J., Clifton, G. L., Young, H. F., Becker, D. P., & Hayes, R. L. (1989). Increased vulnerability of the midly traumatized rat brain to cerebral ischemia: the use of controlled secondary ischemia as a research tool to identify common or different mechanisms contributing to mechanical and ischemic brain injury. Brain Research, 477(1-2), 211-224. https://doi.org/10.1016/0006-8993(89)91409-1