Increased type I procollagen mRNA levels and in vitro protein synthesis in the baboon model of chronic alcoholic liver disease

Mark A Zern, Maria A. Leo, Marie Adele Giambrone, Charles S. Lieber

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

We have investigated the molecular mechanisms responsible for the histologic changes induced by ethanol in the baboon model of alcoholic liver disease. Eleven ethanol-fed baboons and their pair-fed controls had histology evaluated and RNA extracted from percutaneous liver biopsy specimens. In 6 of the ethanol-fed animals, fatty liver developed, but no significant differences were found when the RNA from the control and ethanol-fed livers was translated in the reticulocyte lysate system or analyzed with specific cDNA probes. Five of the baboons given ethanol, however, developed significant fibrosis. Molecular evaluation revealed that the RNA from these livers was more active in in vitro protein synthesis, and the type I procollagen mRNA content was significantly higher per microgram of liver RNA as determined by hybridization analysis (183% ± 23% SEM of control, p < 0.02). In addition, there were higher levels of albumin mRNA content in the livers of ethanol-fed baboons that developed fibrosis (180% ± 21% SEM of control, p < 0.05). There was no change, however, in the levels of β-actin mRNA, a representative constitutive protein. These findings in the baboon model of alcoholic fibrosis show that ethanol consumption (a) increases type I procollagen mRNA, which may foster fibrogenesis; (b) increases albumin mRNA content without causing an increase in serum albumin; and (c) induces no change in levels of β-actin mRNA. These studies also show that percutaneous needle biopsy can supply sufficient tissue to evaluate molecular changes in human liver disease.

Original languageEnglish (US)
Pages (from-to)1123-1131
Number of pages9
JournalGastroenterology
Volume89
Issue number5
StatePublished - 1985
Externally publishedYes

Fingerprint

Alcoholic Liver Diseases
Papio
Collagen Type I
Ethanol
Messenger RNA
Proteins
Liver
RNA
Fibrosis
Actins
Albumins
Reticulocytes
Needle Biopsy
Fatty Liver
In Vitro Techniques
Serum Albumin
Liver Diseases
Histology
Complementary DNA
Biopsy

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Increased type I procollagen mRNA levels and in vitro protein synthesis in the baboon model of chronic alcoholic liver disease. / Zern, Mark A; Leo, Maria A.; Giambrone, Marie Adele; Lieber, Charles S.

In: Gastroenterology, Vol. 89, No. 5, 1985, p. 1123-1131.

Research output: Contribution to journalArticle

Zern, Mark A ; Leo, Maria A. ; Giambrone, Marie Adele ; Lieber, Charles S. / Increased type I procollagen mRNA levels and in vitro protein synthesis in the baboon model of chronic alcoholic liver disease. In: Gastroenterology. 1985 ; Vol. 89, No. 5. pp. 1123-1131.
@article{0918f6ccce7f41089660e80940159e1c,
title = "Increased type I procollagen mRNA levels and in vitro protein synthesis in the baboon model of chronic alcoholic liver disease",
abstract = "We have investigated the molecular mechanisms responsible for the histologic changes induced by ethanol in the baboon model of alcoholic liver disease. Eleven ethanol-fed baboons and their pair-fed controls had histology evaluated and RNA extracted from percutaneous liver biopsy specimens. In 6 of the ethanol-fed animals, fatty liver developed, but no significant differences were found when the RNA from the control and ethanol-fed livers was translated in the reticulocyte lysate system or analyzed with specific cDNA probes. Five of the baboons given ethanol, however, developed significant fibrosis. Molecular evaluation revealed that the RNA from these livers was more active in in vitro protein synthesis, and the type I procollagen mRNA content was significantly higher per microgram of liver RNA as determined by hybridization analysis (183{\%} ± 23{\%} SEM of control, p < 0.02). In addition, there were higher levels of albumin mRNA content in the livers of ethanol-fed baboons that developed fibrosis (180{\%} ± 21{\%} SEM of control, p < 0.05). There was no change, however, in the levels of β-actin mRNA, a representative constitutive protein. These findings in the baboon model of alcoholic fibrosis show that ethanol consumption (a) increases type I procollagen mRNA, which may foster fibrogenesis; (b) increases albumin mRNA content without causing an increase in serum albumin; and (c) induces no change in levels of β-actin mRNA. These studies also show that percutaneous needle biopsy can supply sufficient tissue to evaluate molecular changes in human liver disease.",
author = "Zern, {Mark A} and Leo, {Maria A.} and Giambrone, {Marie Adele} and Lieber, {Charles S.}",
year = "1985",
language = "English (US)",
volume = "89",
pages = "1123--1131",
journal = "Gastroenterology",
issn = "0016-5085",
publisher = "W.B. Saunders Ltd",
number = "5",

}

TY - JOUR

T1 - Increased type I procollagen mRNA levels and in vitro protein synthesis in the baboon model of chronic alcoholic liver disease

AU - Zern, Mark A

AU - Leo, Maria A.

AU - Giambrone, Marie Adele

AU - Lieber, Charles S.

PY - 1985

Y1 - 1985

N2 - We have investigated the molecular mechanisms responsible for the histologic changes induced by ethanol in the baboon model of alcoholic liver disease. Eleven ethanol-fed baboons and their pair-fed controls had histology evaluated and RNA extracted from percutaneous liver biopsy specimens. In 6 of the ethanol-fed animals, fatty liver developed, but no significant differences were found when the RNA from the control and ethanol-fed livers was translated in the reticulocyte lysate system or analyzed with specific cDNA probes. Five of the baboons given ethanol, however, developed significant fibrosis. Molecular evaluation revealed that the RNA from these livers was more active in in vitro protein synthesis, and the type I procollagen mRNA content was significantly higher per microgram of liver RNA as determined by hybridization analysis (183% ± 23% SEM of control, p < 0.02). In addition, there were higher levels of albumin mRNA content in the livers of ethanol-fed baboons that developed fibrosis (180% ± 21% SEM of control, p < 0.05). There was no change, however, in the levels of β-actin mRNA, a representative constitutive protein. These findings in the baboon model of alcoholic fibrosis show that ethanol consumption (a) increases type I procollagen mRNA, which may foster fibrogenesis; (b) increases albumin mRNA content without causing an increase in serum albumin; and (c) induces no change in levels of β-actin mRNA. These studies also show that percutaneous needle biopsy can supply sufficient tissue to evaluate molecular changes in human liver disease.

AB - We have investigated the molecular mechanisms responsible for the histologic changes induced by ethanol in the baboon model of alcoholic liver disease. Eleven ethanol-fed baboons and their pair-fed controls had histology evaluated and RNA extracted from percutaneous liver biopsy specimens. In 6 of the ethanol-fed animals, fatty liver developed, but no significant differences were found when the RNA from the control and ethanol-fed livers was translated in the reticulocyte lysate system or analyzed with specific cDNA probes. Five of the baboons given ethanol, however, developed significant fibrosis. Molecular evaluation revealed that the RNA from these livers was more active in in vitro protein synthesis, and the type I procollagen mRNA content was significantly higher per microgram of liver RNA as determined by hybridization analysis (183% ± 23% SEM of control, p < 0.02). In addition, there were higher levels of albumin mRNA content in the livers of ethanol-fed baboons that developed fibrosis (180% ± 21% SEM of control, p < 0.05). There was no change, however, in the levels of β-actin mRNA, a representative constitutive protein. These findings in the baboon model of alcoholic fibrosis show that ethanol consumption (a) increases type I procollagen mRNA, which may foster fibrogenesis; (b) increases albumin mRNA content without causing an increase in serum albumin; and (c) induces no change in levels of β-actin mRNA. These studies also show that percutaneous needle biopsy can supply sufficient tissue to evaluate molecular changes in human liver disease.

UR - http://www.scopus.com/inward/record.url?scp=0022367508&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022367508&partnerID=8YFLogxK

M3 - Article

C2 - 4043669

AN - SCOPUS:0022367508

VL - 89

SP - 1123

EP - 1131

JO - Gastroenterology

JF - Gastroenterology

SN - 0016-5085

IS - 5

ER -