Abstract
We recently demonstrated that transforming growth factor-β1 stimulates collagen synthesis in hepatic cells in vitro, and that the synthesis of this cytokine is markedly increased in two rodent models of hepatic fibrosis. In the present study, we investigated the association of transforming growth factor-β1 (TFG-β1) gene expression in human liver disease. Sixteen patients with active liver disease had percutaneous liver biopsies performed for diagnostic purposes. Total RNA was extracted from an unused portion of each biopsy and then subjected to hybridization analysis with the following human cDNA clones: albumin, pro α1 (I) collagen, and TGF-β1. Surgical liver biopsy specimens from two patients without hepatic disease were used as controls. When compared to controls, the patients with active liver disease had a 19% decrease in albumin, a 97% increase in type I collagen, and a 120% increase in transforming growth factor-β1 mRNA levels. Moreover, steady-state levels of TGF-β1 and procollagen mRNAs were significantly correlated. Nuclear run-on assays showed that livers from two patients with fibrosis had TGF-β1 transcription rates that were more than 2-fold higher than rates in control livers. These findings indicate that transforming growth factor-β1 gene expression is significantly enhanced in man during active liver disease.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 259-264 |
| Number of pages | 6 |
| Journal | Journal of Hepatology |
| Volume | 14 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Gastroenterology
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Increased transforming growth factor-β1 gene expression in human liver disease. / Annoni, Giorgio; Weiner, Francis R.; Zern, Mark A.
In: Journal of Hepatology, Vol. 14, No. 2-3, 1992, p. 259-264.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Increased transforming growth factor-β1 gene expression in human liver disease
AU - Annoni, Giorgio
AU - Weiner, Francis R.
AU - Zern, Mark A
PY - 1992
Y1 - 1992
N2 - We recently demonstrated that transforming growth factor-β1 stimulates collagen synthesis in hepatic cells in vitro, and that the synthesis of this cytokine is markedly increased in two rodent models of hepatic fibrosis. In the present study, we investigated the association of transforming growth factor-β1 (TFG-β1) gene expression in human liver disease. Sixteen patients with active liver disease had percutaneous liver biopsies performed for diagnostic purposes. Total RNA was extracted from an unused portion of each biopsy and then subjected to hybridization analysis with the following human cDNA clones: albumin, pro α1 (I) collagen, and TGF-β1. Surgical liver biopsy specimens from two patients without hepatic disease were used as controls. When compared to controls, the patients with active liver disease had a 19% decrease in albumin, a 97% increase in type I collagen, and a 120% increase in transforming growth factor-β1 mRNA levels. Moreover, steady-state levels of TGF-β1 and procollagen mRNAs were significantly correlated. Nuclear run-on assays showed that livers from two patients with fibrosis had TGF-β1 transcription rates that were more than 2-fold higher than rates in control livers. These findings indicate that transforming growth factor-β1 gene expression is significantly enhanced in man during active liver disease.
AB - We recently demonstrated that transforming growth factor-β1 stimulates collagen synthesis in hepatic cells in vitro, and that the synthesis of this cytokine is markedly increased in two rodent models of hepatic fibrosis. In the present study, we investigated the association of transforming growth factor-β1 (TFG-β1) gene expression in human liver disease. Sixteen patients with active liver disease had percutaneous liver biopsies performed for diagnostic purposes. Total RNA was extracted from an unused portion of each biopsy and then subjected to hybridization analysis with the following human cDNA clones: albumin, pro α1 (I) collagen, and TGF-β1. Surgical liver biopsy specimens from two patients without hepatic disease were used as controls. When compared to controls, the patients with active liver disease had a 19% decrease in albumin, a 97% increase in type I collagen, and a 120% increase in transforming growth factor-β1 mRNA levels. Moreover, steady-state levels of TGF-β1 and procollagen mRNAs were significantly correlated. Nuclear run-on assays showed that livers from two patients with fibrosis had TGF-β1 transcription rates that were more than 2-fold higher than rates in control livers. These findings indicate that transforming growth factor-β1 gene expression is significantly enhanced in man during active liver disease.
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U2 - 10.1016/0168-8278(92)90168-O
DO - 10.1016/0168-8278(92)90168-O
M3 - Article
VL - 14
SP - 259
EP - 264
JO - Journal of Hepatology
JF - Journal of Hepatology
SN - 0168-8278
IS - 2-3
ER -