Increased neurogenesis in the dentate gyrus after transient global ischemia in gerbils

Jialing Liu, Karen Solway, Robert O. Messing, Frank R Sharp

Research output: Contribution to journalArticle

904 Citations (Scopus)

Abstract

Neurogenesis in the dentate gyrus of adult rodents is regulated by NMDA receptors, adrenal steroids, environmental stimuli, and seizures. To determine whether ischemia affects neurogenesis, newly divided cells in the dentate gyrus were examined after transient global ischemia in adult gerbils. 5-Bromo-2'-deoxyuridine-5'-monophosphate (BrdU) immunohistochemistry demonstrated a 12-fold increase in cell birth in the dentate subgranular zone 1-2 weeks after 10 min bilateral common carotid artery occlusions. Two minutes of ischemia did not significantly increase BrdU incorporation. Confocal microscopy demonstrated that BrdU immunoreactive cells in the granule cell layer colocalized with neuron-specific markers for neuronal nuclear antigen, microtubule-associated protein-2, and calbindin D(28k), indicating that the newly divided cells migrated from the subgranular zone into the granule cell layer and matured into neurons. Newborn cells with a neuronal phenotype were first seen 26 d after ischemia, survived for at least 7 months, were located only in the granule cell layer, and comprised ~60% of BrdU-labeled cells in the granule cell layer 6 weeks after ischemia. The increased neurogenesis was not attributable to entorhinal cortical lesions, because no cell loss was detected in this region. Ischemic preconditioning for 2 min, which protects CA1 neurons against subsequent ischemic damage, did not prevent increased neurogenesis in the granule cell layer after a subsequent severe ischemic challenge. Thus, ischemia-induced dentate neurogenesis is not attributable to CA1 neuronal loss. Enhanced neurogenesis in the dentate gyrus may be a compensatory adaptive response to ischemia- associated injury and could promote functional recovery after ischemic hippocampal injury.

Original languageEnglish (US)
Pages (from-to)7768-7778
Number of pages11
JournalJournal of Neuroscience
Volume18
Issue number19
StatePublished - Oct 1 1998
Externally publishedYes

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Gerbillinae
Neurogenesis
Dentate Gyrus
Ischemia
Bromodeoxyuridine
Neurons
Calbindins
Nuclear Antigens
Ischemic Preconditioning
Microtubule-Associated Proteins
Common Carotid Artery
Wounds and Injuries
N-Methyl-D-Aspartate Receptors
Confocal Microscopy
Rodentia
Seizures
Immunohistochemistry
Steroids
Parturition

Keywords

  • BDNF
  • BrdU
  • CA1
  • Calbindin
  • Cerebral ischemia
  • Dentate gyrus
  • Entorhinal cortex
  • Erythropoietin
  • FGF
  • GFAP
  • Granule neuron
  • Hippocampus
  • MAP-2
  • NeuN
  • Neural stem cells
  • Neurogenesis
  • NMDA receptor
  • Spreading depression
  • Subependyma

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Increased neurogenesis in the dentate gyrus after transient global ischemia in gerbils. / Liu, Jialing; Solway, Karen; Messing, Robert O.; Sharp, Frank R.

In: Journal of Neuroscience, Vol. 18, No. 19, 01.10.1998, p. 7768-7778.

Research output: Contribution to journalArticle

Liu, Jialing ; Solway, Karen ; Messing, Robert O. ; Sharp, Frank R. / Increased neurogenesis in the dentate gyrus after transient global ischemia in gerbils. In: Journal of Neuroscience. 1998 ; Vol. 18, No. 19. pp. 7768-7778.
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