Increased expression of lectinlike oxidized low density lipoprotein receptor-1 in initial atherosclerotic lesions of Watanabe heritable hyperlipidemic rabbits

Mingyi Chen, Makoto Kakutani, Manabu Minami, Hiroharu Kataoka, Noriaki Kume, Shuh Narumiya, Toru Kita, Tomoh Masaki, Tatsuya Sawamura

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

A novel lectinlike oxidized low density lipoprotein receptor-1 (LOX-1) was recently identified in bovine aortic endothelial cells. It is strongly suggested to have a potential role in the initiation and development of atherosclerosis. In this study, we have isolated cDNA clones encoding the rabbit homologue of LOX-1 by screening a rabbit placenta cDNA library. In amino acid sequence and domain structure organization, the rabbit LOX-1 is highly conserved with the human counterpart. Transfection of rabbit LOX-1 cDNA to HEK-293 cells confers on them the activity to bind and internalize oxidized low density lipoprotein. Rabbit LOX-1 was identified as a 45-kDa protein by Western blot analysis with a specific monoclonal antibody. Notably, analyses by reverse transcription-polymerase chain reaction and Western blot revealed that LOX-1 was accumulated in 8-week-old Watanabe heritable hyperlipidemic rabbit aortas compared with normal rabbit aortas. Immunostaining confirmed that the augmented expression of LOX-1 was primarily localized within the intima at the earliest stages of atherogenesis. The most prominent staining was in the endothelial cells of lesions. Furthermore, the distinctive staining of LOX-1 was identified in the endothelium of nonlesion areas of Watanabe heritable hyperlipidemic rabbit aortas. Taken together, these findings support the possibility that LOX-1 might be involved in the initiation of atherosclerosis.

Original languageEnglish (US)
Pages (from-to)1107-1115
Number of pages9
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume20
Issue number4
StatePublished - Apr 2000
Externally publishedYes

Fingerprint

Class E Scavenger Receptors
Rabbits
Aorta
Atherosclerosis
Endothelial Cells
Complementary DNA
Western Blotting
Staining and Labeling
HEK293 Cells
Gene Library
Placenta
Reverse Transcription
Endothelium
Transfection
Amino Acid Sequence
Clone Cells
Monoclonal Antibodies

Keywords

  • Atherosclerosis
  • Endothelium
  • Lectinlike oxidized low density lipoprotein receptor-1
  • Oxidized low density lipoproteins
  • Watanabe heritable hyperlipidemic rabbits

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Increased expression of lectinlike oxidized low density lipoprotein receptor-1 in initial atherosclerotic lesions of Watanabe heritable hyperlipidemic rabbits. / Chen, Mingyi; Kakutani, Makoto; Minami, Manabu; Kataoka, Hiroharu; Kume, Noriaki; Narumiya, Shuh; Kita, Toru; Masaki, Tomoh; Sawamura, Tatsuya.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 20, No. 4, 04.2000, p. 1107-1115.

Research output: Contribution to journalArticle

Chen, M, Kakutani, M, Minami, M, Kataoka, H, Kume, N, Narumiya, S, Kita, T, Masaki, T & Sawamura, T 2000, 'Increased expression of lectinlike oxidized low density lipoprotein receptor-1 in initial atherosclerotic lesions of Watanabe heritable hyperlipidemic rabbits', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 20, no. 4, pp. 1107-1115.
Chen, Mingyi ; Kakutani, Makoto ; Minami, Manabu ; Kataoka, Hiroharu ; Kume, Noriaki ; Narumiya, Shuh ; Kita, Toru ; Masaki, Tomoh ; Sawamura, Tatsuya. / Increased expression of lectinlike oxidized low density lipoprotein receptor-1 in initial atherosclerotic lesions of Watanabe heritable hyperlipidemic rabbits. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2000 ; Vol. 20, No. 4. pp. 1107-1115.
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