Increased clonogenic (CFU-C, PFU-C) populations from bone marrow and spleen of nude mice

Floyd D. Wilson, M. Eric Gershwin, Moshe Shifrine, Ruth Graham

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Clonogenic populations from bone marrow and spleen of nude mice and their normal littermates were enumerated in vitro using a methylcellulose supported culture system. This technique allows for the simultaneous quantitation of progenitors of granulocyte-monocyte pathways (colony forming units in culture, CFU-C) and for progenitors of "mesenchymal" elements (plaque-forming units in culture or PFU-C). These populations were distinguished in culture by their growth characteristics and morphology. CFU-C gave rise to suspended colonies of granulocyte-monocyte composition while PFU-C formed surface-adherent colonies of mesenchymal morphological features (fibroblastic and reticuloendothelial morphology). Significant elevations in the relative and absolute numbers of CFU-C and PFU-C were observed in the bone marrow and spleen of 6 wk old nu/nu mice relative to heterozygous littermates. The results are discussed in terms of non-T cell components involved in cell-mediated immunity against neoplastic development.

Original languageEnglish (US)
Pages (from-to)373-383
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume1
Issue number4
DOIs
StatePublished - 1977

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

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