Increased biosynthesis of lipoxygenase products by UVB-irradiated guinea pig epidermis

Evidence of a cyclooxygenase inhibitor

Barbara A Burrall, Vincent A. Ziboh

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12 Citations (Scopus)

Abstract

The present studies demonstrate that incubation of arachidonic acid (AA) with a 20,000 g homogenate (containing both microsomal and cytoplasmic fractions) from UVB-irradiated guinea pig epidermis (24-72 h) resulted in decreased transformation of the [14c]AA into the cyclooxygenase products (PGD2, PGE2, and PGF2) while the incorporation of 14C into lipoxygenase products (15-HETE and 12-HETE) increased. An investigation into the selective inhibition of the cyclooxygenase pathway revealed that the in vitro transformation of [14c]AA into [14c]-cyclooxygenase products by the 100,000g particulate fraction prepared from normal unirradiated guinea pig epidermis was inhibited by the 100,000 g cytoplasmic extract prepared from a 24-h postirradiated guinea pig epidermis. These latter data imply that an endogenous inhibitor of the cyclooxygenase pathway is generated and released into the cytoplasm during UVB irradiation and it is likely that this selective inhibition of the cyclooxygenase pathway may contribute at least in part to the increased lipoxygenase products in the 24-h postirradiated skin specimens and possibly the recognized prolonged UVB-induced inflammatory process.

Original languageEnglish (US)
Pages (from-to)643-648
Number of pages6
JournalJournal of Investigative Dermatology
Volume86
Issue number6
StatePublished - Jun 1986

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Lipoxygenase
Cyclooxygenase Inhibitors
Biosynthesis
Prostaglandin-Endoperoxide Synthases
Epidermis
Guinea Pigs
Arachidonic Acid
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Prostaglandin D2
Dinoprost
Dinoprostone
Skin
Cytoplasm
Irradiation

ASJC Scopus subject areas

  • Dermatology

Cite this

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title = "Increased biosynthesis of lipoxygenase products by UVB-irradiated guinea pig epidermis: Evidence of a cyclooxygenase inhibitor",
abstract = "The present studies demonstrate that incubation of arachidonic acid (AA) with a 20,000 g homogenate (containing both microsomal and cytoplasmic fractions) from UVB-irradiated guinea pig epidermis (24-72 h) resulted in decreased transformation of the [14c]AA into the cyclooxygenase products (PGD2, PGE2, and PGF2) while the incorporation of 14C into lipoxygenase products (15-HETE and 12-HETE) increased. An investigation into the selective inhibition of the cyclooxygenase pathway revealed that the in vitro transformation of [14c]AA into [14c]-cyclooxygenase products by the 100,000g particulate fraction prepared from normal unirradiated guinea pig epidermis was inhibited by the 100,000 g cytoplasmic extract prepared from a 24-h postirradiated guinea pig epidermis. These latter data imply that an endogenous inhibitor of the cyclooxygenase pathway is generated and released into the cytoplasm during UVB irradiation and it is likely that this selective inhibition of the cyclooxygenase pathway may contribute at least in part to the increased lipoxygenase products in the 24-h postirradiated skin specimens and possibly the recognized prolonged UVB-induced inflammatory process.",
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T1 - Increased biosynthesis of lipoxygenase products by UVB-irradiated guinea pig epidermis

T2 - Evidence of a cyclooxygenase inhibitor

AU - Burrall, Barbara A

AU - Ziboh, Vincent A.

PY - 1986/6

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N2 - The present studies demonstrate that incubation of arachidonic acid (AA) with a 20,000 g homogenate (containing both microsomal and cytoplasmic fractions) from UVB-irradiated guinea pig epidermis (24-72 h) resulted in decreased transformation of the [14c]AA into the cyclooxygenase products (PGD2, PGE2, and PGF2) while the incorporation of 14C into lipoxygenase products (15-HETE and 12-HETE) increased. An investigation into the selective inhibition of the cyclooxygenase pathway revealed that the in vitro transformation of [14c]AA into [14c]-cyclooxygenase products by the 100,000g particulate fraction prepared from normal unirradiated guinea pig epidermis was inhibited by the 100,000 g cytoplasmic extract prepared from a 24-h postirradiated guinea pig epidermis. These latter data imply that an endogenous inhibitor of the cyclooxygenase pathway is generated and released into the cytoplasm during UVB irradiation and it is likely that this selective inhibition of the cyclooxygenase pathway may contribute at least in part to the increased lipoxygenase products in the 24-h postirradiated skin specimens and possibly the recognized prolonged UVB-induced inflammatory process.

AB - The present studies demonstrate that incubation of arachidonic acid (AA) with a 20,000 g homogenate (containing both microsomal and cytoplasmic fractions) from UVB-irradiated guinea pig epidermis (24-72 h) resulted in decreased transformation of the [14c]AA into the cyclooxygenase products (PGD2, PGE2, and PGF2) while the incorporation of 14C into lipoxygenase products (15-HETE and 12-HETE) increased. An investigation into the selective inhibition of the cyclooxygenase pathway revealed that the in vitro transformation of [14c]AA into [14c]-cyclooxygenase products by the 100,000g particulate fraction prepared from normal unirradiated guinea pig epidermis was inhibited by the 100,000 g cytoplasmic extract prepared from a 24-h postirradiated guinea pig epidermis. These latter data imply that an endogenous inhibitor of the cyclooxygenase pathway is generated and released into the cytoplasm during UVB irradiation and it is likely that this selective inhibition of the cyclooxygenase pathway may contribute at least in part to the increased lipoxygenase products in the 24-h postirradiated skin specimens and possibly the recognized prolonged UVB-induced inflammatory process.

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