Increase in the advanced glycation end product pentosidine in Bruch's membrane with age

James T. Handa, Nicole Verzijl, Hiroshi Matsunaga, Amy Aotaki-Keen, Gerard A. Lutty, Johan M. Te Koppele, Toshio Miyata, Leonard M Hjelmeland

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Abstract

PURPOSE. To determine whether there is an age-related increase of pentosidine in human Bruch's membranes and to localize pentosidine and carboxymethyllysine (CML), two well-characterized, advanced glycation end products (AGEs) in aged human Bruch's membranes and choroid in vivo. METHODS. Human Bruch's membrane samples were isolated from the retinal pigment epithelium (RPE) and choroid and subjected to reversed-phase high-performance liquid chromatography to determine pentosidine content. A polyclonal anti- pentosidine antibody and a monoclonal antibody specific for carboxymethyllysine were used to localize AGEs in 20-month-old nondiabetic, 82-year-old nondiabetic, and 82-year-old diabetic globes. RESULTS. Human Bruch's membranes (n = 20) showed a linear age-dependent increase in pentosidine that reached approximately 0.17 millimoles pentosidine per mole hydroxyproline in late life (r = 0.896; P < 0.001). Immunohistochemical evaluation showed evidence of pentosidine in Bruch's membrane, choroidal extracellular matrix, and vessel walls in the 82-year-old nondiabetic and diabetic globes. A similar staining pattern was found with the anti-CML antibody. Basal laminar deposits and drusen stained with both antibodies in the elderly nondiabetic eye. In contrast, neither antibody stained the 20- month-old tissue. CONCLUSIONS. We provide biochemical and immunohistochemical evidence for the formation of pentosidine and CML structures in human Bruch's membrane and choroid with age. These changes could promote aging of the RPE- Bruch's membrane-choroid complex.

Original languageEnglish (US)
Pages (from-to)775-779
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume40
Issue number3
StatePublished - 1999

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Bruch Membrane
Advanced Glycosylation End Products
Choroid
Retinal Pigment Epithelium
Anti-Idiotypic Antibodies
pentosidine
Antibodies
Hydroxyproline
Reverse-Phase Chromatography
Extracellular Matrix
High Pressure Liquid Chromatography
Monoclonal Antibodies
Staining and Labeling
N(6)-carboxymethyllysine

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Handa, J. T., Verzijl, N., Matsunaga, H., Aotaki-Keen, A., Lutty, G. A., Te Koppele, J. M., ... Hjelmeland, L. M. (1999). Increase in the advanced glycation end product pentosidine in Bruch's membrane with age. Investigative Ophthalmology and Visual Science, 40(3), 775-779.

Increase in the advanced glycation end product pentosidine in Bruch's membrane with age. / Handa, James T.; Verzijl, Nicole; Matsunaga, Hiroshi; Aotaki-Keen, Amy; Lutty, Gerard A.; Te Koppele, Johan M.; Miyata, Toshio; Hjelmeland, Leonard M.

In: Investigative Ophthalmology and Visual Science, Vol. 40, No. 3, 1999, p. 775-779.

Research output: Contribution to journalArticle

Handa, JT, Verzijl, N, Matsunaga, H, Aotaki-Keen, A, Lutty, GA, Te Koppele, JM, Miyata, T & Hjelmeland, LM 1999, 'Increase in the advanced glycation end product pentosidine in Bruch's membrane with age', Investigative Ophthalmology and Visual Science, vol. 40, no. 3, pp. 775-779.
Handa JT, Verzijl N, Matsunaga H, Aotaki-Keen A, Lutty GA, Te Koppele JM et al. Increase in the advanced glycation end product pentosidine in Bruch's membrane with age. Investigative Ophthalmology and Visual Science. 1999;40(3):775-779.
Handa, James T. ; Verzijl, Nicole ; Matsunaga, Hiroshi ; Aotaki-Keen, Amy ; Lutty, Gerard A. ; Te Koppele, Johan M. ; Miyata, Toshio ; Hjelmeland, Leonard M. / Increase in the advanced glycation end product pentosidine in Bruch's membrane with age. In: Investigative Ophthalmology and Visual Science. 1999 ; Vol. 40, No. 3. pp. 775-779.
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T1 - Increase in the advanced glycation end product pentosidine in Bruch's membrane with age

AU - Handa, James T.

AU - Verzijl, Nicole

AU - Matsunaga, Hiroshi

AU - Aotaki-Keen, Amy

AU - Lutty, Gerard A.

AU - Te Koppele, Johan M.

AU - Miyata, Toshio

AU - Hjelmeland, Leonard M

PY - 1999

Y1 - 1999

N2 - PURPOSE. To determine whether there is an age-related increase of pentosidine in human Bruch's membranes and to localize pentosidine and carboxymethyllysine (CML), two well-characterized, advanced glycation end products (AGEs) in aged human Bruch's membranes and choroid in vivo. METHODS. Human Bruch's membrane samples were isolated from the retinal pigment epithelium (RPE) and choroid and subjected to reversed-phase high-performance liquid chromatography to determine pentosidine content. A polyclonal anti- pentosidine antibody and a monoclonal antibody specific for carboxymethyllysine were used to localize AGEs in 20-month-old nondiabetic, 82-year-old nondiabetic, and 82-year-old diabetic globes. RESULTS. Human Bruch's membranes (n = 20) showed a linear age-dependent increase in pentosidine that reached approximately 0.17 millimoles pentosidine per mole hydroxyproline in late life (r = 0.896; P < 0.001). Immunohistochemical evaluation showed evidence of pentosidine in Bruch's membrane, choroidal extracellular matrix, and vessel walls in the 82-year-old nondiabetic and diabetic globes. A similar staining pattern was found with the anti-CML antibody. Basal laminar deposits and drusen stained with both antibodies in the elderly nondiabetic eye. In contrast, neither antibody stained the 20- month-old tissue. CONCLUSIONS. We provide biochemical and immunohistochemical evidence for the formation of pentosidine and CML structures in human Bruch's membrane and choroid with age. These changes could promote aging of the RPE- Bruch's membrane-choroid complex.

AB - PURPOSE. To determine whether there is an age-related increase of pentosidine in human Bruch's membranes and to localize pentosidine and carboxymethyllysine (CML), two well-characterized, advanced glycation end products (AGEs) in aged human Bruch's membranes and choroid in vivo. METHODS. Human Bruch's membrane samples were isolated from the retinal pigment epithelium (RPE) and choroid and subjected to reversed-phase high-performance liquid chromatography to determine pentosidine content. A polyclonal anti- pentosidine antibody and a monoclonal antibody specific for carboxymethyllysine were used to localize AGEs in 20-month-old nondiabetic, 82-year-old nondiabetic, and 82-year-old diabetic globes. RESULTS. Human Bruch's membranes (n = 20) showed a linear age-dependent increase in pentosidine that reached approximately 0.17 millimoles pentosidine per mole hydroxyproline in late life (r = 0.896; P < 0.001). Immunohistochemical evaluation showed evidence of pentosidine in Bruch's membrane, choroidal extracellular matrix, and vessel walls in the 82-year-old nondiabetic and diabetic globes. A similar staining pattern was found with the anti-CML antibody. Basal laminar deposits and drusen stained with both antibodies in the elderly nondiabetic eye. In contrast, neither antibody stained the 20- month-old tissue. CONCLUSIONS. We provide biochemical and immunohistochemical evidence for the formation of pentosidine and CML structures in human Bruch's membrane and choroid with age. These changes could promote aging of the RPE- Bruch's membrane-choroid complex.

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