Inactivation of glutathione S-transferases by nitric oxide-derived oxidants

Exploring a role for tyrosine nitration

P. S Y Wong, J. P. Eiserich, S. Reddy, C. L. Lopez, Carroll E Cross, A. Van der Vliet

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Reactive intermediates derived from nitric oxide (·NO) are thought to play a contributing role in disease states associated with inflammation and infection. We show here that glutathione S-transferases (GSTs), principal enzymes responsible for detoxification of endogenous and exogenous electrophiles, are susceptible to inactivation by reactive nitrogen species (RNS). Treatment of isolated GSTs or rat liver homogenates with either peroxynitrite, the myeloperoxidase/hydrogen peroxide/nitrite system, or tetranitromethane, resulted in loss of GST activity with a concomitant increase in the formation of protein-associated 3-nitrotyrosine (NO2Tyr). This inactivation was only partially (<25%) reversible by dithiothreitol, and exposure of GSTs to hydrogen peroxide or S-nitroso-glutathione was only partially inhibitory (<25%) and did not result in protein nitration. Thus, irreversible modifications such as tyrosine nitration may have contributed to GST inactivation by RNS. Since all GSTs contain a critical, highly conserved, active-site tyrosine residue, we postulated that this Tyr residue might present a primary target for nitration by RNS, thus leading to enzyme inactivation. To directly investigate this possibility, we analyzed purified mouse liver GST-μ, following nitration by several RNS, by trypsin digestion, HPLC separation, and matrix-assisted laser desorption/ionization-time of flight analysis, to determine the degree of tyrosine nitration of individual Tyr residues. Indeed, nitration was found to occur preferentially on several tyrosine residues located in and around the GST active site. However, RNS concentrations that resulted in near complete GST inactivation only caused up to 25% nitration of even preferentially targeted tyrosine residues. Hence, nitration of active-site tyrosine residues may contribute to GST inactivation by RNS, but is unlikely to fully account for enzyme inactivation. Overall, our studies illustrate a potential mechanism by which RNS may promote (oxidative) injury by environmental pollutants in association with inflammation.

Original languageEnglish (US)
Pages (from-to)216-228
Number of pages13
JournalArchives of Biochemistry and Biophysics
Volume394
Issue number2
DOIs
StatePublished - Oct 15 2001

Fingerprint

Nitration
Glutathione Transferase
Oxidants
Reactive Nitrogen Species
Tyrosine
Nitric Oxide
Catalytic Domain
Liver
Hydrogen Peroxide
Enzymes
Tetranitromethane
Inflammation
Environmental Pollutants
Detoxification
Peroxynitrous Acid
Dithiothreitol
Nitrites
Trypsin
Peroxidase
Ionization

Keywords

  • Glutathione S-transferase
  • Inflammation
  • Myeloperoxidase
  • Nitric oxide
  • Peroxynitrite
  • Protein oxidation
  • Tyrosine nitration

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Inactivation of glutathione S-transferases by nitric oxide-derived oxidants : Exploring a role for tyrosine nitration. / Wong, P. S Y; Eiserich, J. P.; Reddy, S.; Lopez, C. L.; Cross, Carroll E; Van der Vliet, A.

In: Archives of Biochemistry and Biophysics, Vol. 394, No. 2, 15.10.2001, p. 216-228.

Research output: Contribution to journalArticle

Wong, P. S Y ; Eiserich, J. P. ; Reddy, S. ; Lopez, C. L. ; Cross, Carroll E ; Van der Vliet, A. / Inactivation of glutathione S-transferases by nitric oxide-derived oxidants : Exploring a role for tyrosine nitration. In: Archives of Biochemistry and Biophysics. 2001 ; Vol. 394, No. 2. pp. 216-228.
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