Inactivation of Cdh1 by synergistic action of Cdk1 and polo kinase is necessary for proper assembly of the mitotic spindle

Karen Crasta, Hong Hwa Lim, Thomas H. Giddings, Mark Winey, Uttam Surana

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Separation of duplicated centrosomes (spindle-pole bodies or SPBs in yeast) is a crucial step in the biogenesis of the mitotic spindle. In vertebrates, centrosome separation requires the BimC family kinesin Eg5 and the activities of Cdk1 and polo kinase; however, the roles of these kinases are not fully understood. In Saccharomyces cerevisiae, SPB separation also requires activated Cdk1 and the plus-end kinesins Cin8 (homologous to vertebrate Eg5) and Kip1. Here we report that polo kinase has a role in the separation of SPBs. We show that adequate accumulation of Cin8 and Kip1 requires inactivation of the anaphase-promoting complex-activator Cdh1 through sequential phosphorylation by Cdk1 and polo kinase. In this process, Cdk1 functions as a priming kinase in that Cdk1-mediated phosphorylation creates a binding site for polo kinase,which further phosphorylates Cdh1. Thus, Cdh1 inactivation through the synergistic action of Cdk1 and polo kinase provides a new model for inactivation of cell-cycle effectors.

Original languageEnglish (US)
Pages (from-to)665-675
Number of pages11
JournalNature Cell Biology
Volume10
Issue number6
DOIs
StatePublished - Jun 1 2008
Externally publishedYes

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CDC2 Protein Kinase
Spindle Apparatus
Kinesin
Centrosome
Phosphotransferases
Vertebrates
Spindle Pole Bodies
Phosphorylation
Anaphase-Promoting Complex-Cyclosome
Saccharomyces cerevisiae
Cell Cycle
Yeasts
Binding Sites

ASJC Scopus subject areas

  • Cell Biology

Cite this

Inactivation of Cdh1 by synergistic action of Cdk1 and polo kinase is necessary for proper assembly of the mitotic spindle. / Crasta, Karen; Lim, Hong Hwa; Giddings, Thomas H.; Winey, Mark; Surana, Uttam.

In: Nature Cell Biology, Vol. 10, No. 6, 01.06.2008, p. 665-675.

Research output: Contribution to journalArticle

Crasta, Karen ; Lim, Hong Hwa ; Giddings, Thomas H. ; Winey, Mark ; Surana, Uttam. / Inactivation of Cdh1 by synergistic action of Cdk1 and polo kinase is necessary for proper assembly of the mitotic spindle. In: Nature Cell Biology. 2008 ; Vol. 10, No. 6. pp. 665-675.
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