Inability of real-time reverse transcriptase PCR assay to detect subtype H7 avian influenza viruses isolated from wild birds

Zheng Xing, Carol Cardona, Phuong Dao, Beate Crossley, Sharon Hietala, Walter M Boyce

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

We report a failure of the real-time reverse transcriptase PCR H7 subtyping protocol currently used in national avian influenza surveillance programs. Significant substitutions in primer and probe target sequences were identified, especially in wild bird viruses. The protocol, originally designed for detecting H7 influenza viruses in poultry, is not reliable for wild bird surveillance.

Original languageEnglish (US)
Pages (from-to)1844-1846
Number of pages3
JournalJournal of Clinical Microbiology
Volume46
Issue number5
DOIs
StatePublished - May 2008

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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