Inability of an isogenic urease-negative mutant strain of Helicobacter mustelae to colonize the ferret stomach

K. A. Andrutis, J. G. Fox, D. B. Schauer, R. P. Marini, J. C. Murphy, L. Yan, Jay V Solnick

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Abstract

Eight ferrets specific-pathogen-free for Helicobacter mustelae were given, per dose, approximately 3.0 x 107 CFU of either the wild-type parent strain of H. mustelae (NCTC 12032) (two ferrets), the isogenic urease-negative mutant strain of H. mustelae (10::Tn3Km) (four ferrets), or sterile culture broth (two ferrets). Infection status was monitored by endoscopic gastric biopsy for urease activity, histopathology, and culture and by serology at 3, 6, 10, and 21 weeks. All ferrets were necropsied at 25 weeks. Both negative control ferrets remained uninfected, both ferrets receiving the H. mustelae wild-type parent strain became infected after two doses of the organism, and all four ferrets given two doses of the isogenic urease-negative mutant strain of H. mustelae remained uninfected throughout the 6-month study. Histopathology correlated with infection status. H. mustelae-infected ferrets exhibited diffuse mononuclear inflammation in the subglandular region and the lamina propria of the gastric mucosa, while uninfected ferrets showed no or minimal inflammation. These results suggest that urease activity is essential for colonization of the ferret stomach by H. mustelae.

Original languageEnglish (US)
Pages (from-to)3722-3725
Number of pages4
JournalInfection and Immunity
Volume63
Issue number9
StatePublished - 1995
Externally publishedYes

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Helicobacter mustelae
Ferrets
Urease
Stomach
Inflammation
Specific Pathogen-Free Organisms
Serology
Gastric Mucosa
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Andrutis, K. A., Fox, J. G., Schauer, D. B., Marini, R. P., Murphy, J. C., Yan, L., & Solnick, J. V. (1995). Inability of an isogenic urease-negative mutant strain of Helicobacter mustelae to colonize the ferret stomach. Infection and Immunity, 63(9), 3722-3725.

Inability of an isogenic urease-negative mutant strain of Helicobacter mustelae to colonize the ferret stomach. / Andrutis, K. A.; Fox, J. G.; Schauer, D. B.; Marini, R. P.; Murphy, J. C.; Yan, L.; Solnick, Jay V.

In: Infection and Immunity, Vol. 63, No. 9, 1995, p. 3722-3725.

Research output: Contribution to journalArticle

Andrutis, KA, Fox, JG, Schauer, DB, Marini, RP, Murphy, JC, Yan, L & Solnick, JV 1995, 'Inability of an isogenic urease-negative mutant strain of Helicobacter mustelae to colonize the ferret stomach', Infection and Immunity, vol. 63, no. 9, pp. 3722-3725.
Andrutis KA, Fox JG, Schauer DB, Marini RP, Murphy JC, Yan L et al. Inability of an isogenic urease-negative mutant strain of Helicobacter mustelae to colonize the ferret stomach. Infection and Immunity. 1995;63(9):3722-3725.
Andrutis, K. A. ; Fox, J. G. ; Schauer, D. B. ; Marini, R. P. ; Murphy, J. C. ; Yan, L. ; Solnick, Jay V. / Inability of an isogenic urease-negative mutant strain of Helicobacter mustelae to colonize the ferret stomach. In: Infection and Immunity. 1995 ; Vol. 63, No. 9. pp. 3722-3725.
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