In vivo volumetric imaging of human retinal circulation with phase-variance optical coherence tomography

Dae Yu Kim, Jeff Fingler, John S Werner, Daniel M. Schwartz, Scott E. Fraser, Robert Zawadzki

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

We present in vivo volumetric images of human retinal micro-circulation using Fourier-domain optical coherence tomography (Fd-OCT) with the phase-variance based motion contrast method. Currently fundus fluorescein angiography (FA) is the standard technique in clinical settings for visualizing blood circulation of the retina. High contrast imaging of retinal vasculature is achieved by injection of a fluorescein dye into the systemic circulation. We previously reported phase-variance optical coherence tomography (pvOCT) as an alternative and non-invasive technique to image human retinal capillaries. In contrast to FA, pvOCT allows not only noninvasive visualization of a two-dimensional retinal perfusion map but also volumetric morphology of retinal microvasculature with high sensitivity. In this paper we report high-speed acquisition at 125 kHz A-scans with pvOCT to reduce motion artifacts and increase the scanning area when compared with previous reports. Two scanning schemes with different sampling densities and scanning areas are evaluated to find optimal parameters for high acquisition speed in vivo imaging. In order to evaluate this technique, we compare pvOCT capillary imaging at 3×3 mm2 and 1.5×1.5 mm2 with fundus FA for a normal human subject. Additionally, a volumetric view of retinal capillaries and a stitched image acquired with ten 3×3 mm2 pvOCT sub-volumes are presented. Visualization of retinal vasculature with pvOCT has potential for diagnosis of retinal vascular diseases.

Original languageEnglish (US)
Pages (from-to)1504-1513
Number of pages10
JournalBiomedical Optics Express
Volume2
Issue number6
DOIs
StatePublished - 2011

Fingerprint

Optical Coherence Tomography
tomography
angiography
Fluorescein Angiography
scanning
acquisition
blood circulation
Retinal Diseases
Retinal Vessels
retina
Blood Circulation
Microvessels
Fluorescein
Vascular Diseases
Artifacts
Retina
artifacts
Coloring Agents
Perfusion
dyes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

Cite this

In vivo volumetric imaging of human retinal circulation with phase-variance optical coherence tomography. / Kim, Dae Yu; Fingler, Jeff; Werner, John S; Schwartz, Daniel M.; Fraser, Scott E.; Zawadzki, Robert.

In: Biomedical Optics Express, Vol. 2, No. 6, 2011, p. 1504-1513.

Research output: Contribution to journalArticle

Kim, Dae Yu ; Fingler, Jeff ; Werner, John S ; Schwartz, Daniel M. ; Fraser, Scott E. ; Zawadzki, Robert. / In vivo volumetric imaging of human retinal circulation with phase-variance optical coherence tomography. In: Biomedical Optics Express. 2011 ; Vol. 2, No. 6. pp. 1504-1513.
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