In-vivo validation of fluorescence lifetime imaging (FLIm) of coronary arteries in swine

Julien Bec, Dinglong Ma, Diego R. Yankelevich, Dimitris Gorpas, William T. Ferrier, Jeffrey Southard, Laura Marcu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

We report a scanning imaging system that enables high speed multispectral fluorescence lifetime imaging (FLIm) of coronary arteries. This system combines a custom low profile (3 Fr) imaging catheter using a 200 μm core side viewing UV-grade silica fiber optic, an acquisition system able to measure fluorescence decays over four spectral bands at 20 kHz and a fast data analysis and display module. In vivo use of the system has been optimized, with particular emphasis on clearing blood from the optical pathway. A short acquisition time (5 seconds for a 20 mm long coronary segment) enabled data acquisition during a bolus saline solution injection through the 7 Fr catheter guide. The injection parameters were precisely controlled using a power injector and optimized to provide good image quality while limiting the bolus injection duration and volume (12 cc/s, 80 cc total volume). The ability of the system to acquire data in vivo was validated in healthy swine by imaging different sections of the left anterior descending (LAD) coronary. A stent coated with fluorescent markers was placed in the LAD and imaged, demonstrating the ability of the system to discriminate in vivo different fluorescent features and structures from the vessel background fluorescence using spectral and lifetime information. Intensity en face images over the four bands of the instrument were available within seconds whereas lifetime images were computed in 2 minutes, providing efficient feedback during the procedure. This successful demonstration of FLIm in coronaries enables future study of atherosclerotic cardiovascular diseases.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume9303
ISBN (Print)9781628413939
DOIs
StatePublished - 2015
EventPhotonic Therapeutics and Diagnostics XI - San Francisco, United States
Duration: Feb 7 2015Feb 8 2015

Other

OtherPhotonic Therapeutics and Diagnostics XI
CountryUnited States
CitySan Francisco
Period2/7/152/8/15

Fingerprint

swine
Optical Imaging
arteries
Coronary Vessels
Aptitude
Swine
Fluorescence
Imaging techniques
life (durability)
fluorescence
Injections
Catheters
injection
Data Display
acquisition
Sodium Chloride
Silicon Dioxide
Stents
clearing
Cardiovascular Diseases

Keywords

  • Atherosclerosis
  • Fluorescence lifetime imaging
  • Intravascular catheter
  • Time resolved fluorescence spectroscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Bec, J., Ma, D., Yankelevich, D. R., Gorpas, D., Ferrier, W. T., Southard, J., & Marcu, L. (2015). In-vivo validation of fluorescence lifetime imaging (FLIm) of coronary arteries in swine. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 9303). [93033M] SPIE. https://doi.org/10.1117/12.2079833

In-vivo validation of fluorescence lifetime imaging (FLIm) of coronary arteries in swine. / Bec, Julien; Ma, Dinglong; Yankelevich, Diego R.; Gorpas, Dimitris; Ferrier, William T.; Southard, Jeffrey; Marcu, Laura.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9303 SPIE, 2015. 93033M.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Bec, J, Ma, D, Yankelevich, DR, Gorpas, D, Ferrier, WT, Southard, J & Marcu, L 2015, In-vivo validation of fluorescence lifetime imaging (FLIm) of coronary arteries in swine. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 9303, 93033M, SPIE, Photonic Therapeutics and Diagnostics XI, San Francisco, United States, 2/7/15. https://doi.org/10.1117/12.2079833
Bec J, Ma D, Yankelevich DR, Gorpas D, Ferrier WT, Southard J et al. In-vivo validation of fluorescence lifetime imaging (FLIm) of coronary arteries in swine. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9303. SPIE. 2015. 93033M https://doi.org/10.1117/12.2079833
Bec, Julien ; Ma, Dinglong ; Yankelevich, Diego R. ; Gorpas, Dimitris ; Ferrier, William T. ; Southard, Jeffrey ; Marcu, Laura. / In-vivo validation of fluorescence lifetime imaging (FLIm) of coronary arteries in swine. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9303 SPIE, 2015.
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