TY - JOUR
T1 - In vivo validation of a bimodal technique combining time-resolved fluorescence spectroscopy and ultrasonic backscatter microscopy for diagnosis of oral carcinoma
AU - Sun, Yang
AU - Xie, Hongtao
AU - Liu, Jing
AU - Lam, Matthew
AU - Chaudhari, Abhijit
AU - Zhou, Feifei
AU - Bec, Julien
AU - Yankelevich, Diego R.
AU - Dobbie, Allison
AU - Tinling, Steven L.
AU - Gandour-Edwards, Regina F
AU - Monsky, Wayne L.
AU - Farwell, D Gregory
AU - Marcu, Laura
PY - 2012/11
Y1 - 2012/11
N2 - Tissue diagnostic features generated by a bimodal technique integrating scanning time-resolved fluorescence spectroscopy (TRFS) and ultrasonic backscatter microscopy (UBM) are investigated in an in vivo hamster oral carcinoma model. Tissue fluorescence is excited by a pulsed nitrogen laser and spectrally and temporally resolved using a set of filters/dichroic mirrors and a fast digitizer, respectively. A 41-MHz focused transducer (37-μm axial, 65-μm lateral resolution) is used for UBM scanning. Representative lesions of the different stages of carcinogenesis show that fluorescence characteristics complement ultrasonic features, and both correlate with histological findings. These results demonstrate that TRFS-UBM provide a wealth of co-registered, complementary data concerning tissue composition and structure as it relates to disease status. The direct co-registration of the TRFS data (sensitive to surface molecular changes) with the UBM data (sensitive to cross-sectional structural changes and depth of tumor invasion) is expected to play an important role in pre-operative diagnosis and intraoperative determination of tumor margins.
AB - Tissue diagnostic features generated by a bimodal technique integrating scanning time-resolved fluorescence spectroscopy (TRFS) and ultrasonic backscatter microscopy (UBM) are investigated in an in vivo hamster oral carcinoma model. Tissue fluorescence is excited by a pulsed nitrogen laser and spectrally and temporally resolved using a set of filters/dichroic mirrors and a fast digitizer, respectively. A 41-MHz focused transducer (37-μm axial, 65-μm lateral resolution) is used for UBM scanning. Representative lesions of the different stages of carcinogenesis show that fluorescence characteristics complement ultrasonic features, and both correlate with histological findings. These results demonstrate that TRFS-UBM provide a wealth of co-registered, complementary data concerning tissue composition and structure as it relates to disease status. The direct co-registration of the TRFS data (sensitive to surface molecular changes) with the UBM data (sensitive to cross-sectional structural changes and depth of tumor invasion) is expected to play an important role in pre-operative diagnosis and intraoperative determination of tumor margins.
KW - lifetime imaging
KW - multimodal diagnosis of cancer
KW - oral carcinoma
KW - time-resolved fluorescence spectroscopy
KW - tissue diagnosis
KW - ultrasonic backscatter microscopy
UR - http://www.scopus.com/inward/record.url?scp=84870580651&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84870580651&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.17.11.116003
DO - 10.1117/1.JBO.17.11.116003
M3 - Article
C2 - 23117798
AN - SCOPUS:84870580651
VL - 17
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 11
M1 - 116003
ER -