In Vivo Tracking of Copper-64 Radiolabeled Nanoparticles in Lactuca sativa

Ryan A. Davis, Devin A. Rippner, Sven H. Hausner, Sanjai J. Parikh, Andrew J. McElrone, Julie Sutcliffe

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Engineered nanoparticles (NPs) are increasingly used in commercial products including automotive lubricants, clothing, deodorants, sunscreens, and cosmetics and can potentially accumulate in our food supply. Given their size it is difficult to detect and visualize the presence of NPs in environmental samples, including crop plants. New analytical tools are needed to fill the void for detection and visualization of NPs in complex biological and environmental matrices. We aimed to determine whether radiolabeled NPs could be used as a noninvasive, highly sensitive analytical tool to quantitatively track and visualize NP transport and accumulation in vivo in lettuce (Lactuca sativa) and to investigate the effect of NP size on transport and distribution over time using a combination of autoradiography, positron emission tomography (PET)/computed tomography (CT), scanning electron microscopy (SEM), and transition electron microscopy (TEM). Azide functionalized NPs were radiolabeled via a "click" reaction with copper-64 (64Cu)-1,4,7-triazacyclononane triacetic acid (NOTA) azadibenzocyclooctyne (ADIBO) conjugate ([64Cu]-ADIBO-NOTA) via copper-free Huisgen-1,3-dipolar cycloaddition reaction. This yielded radiolabeled [64Cu]-NPs of uniform shape and size with a high radiochemical purity (>99%), specific activity of 2.2 mCi/mg of NP, and high stability (i.e., no detectable dissolution) over 24 h across a pH range of 5-9. Both PET/CT and autoradiography showed that [64Cu]-NPs entered the lettuce seedling roots and were rapidly transported to the cotyledons with the majority of the accumulation inside the roots. Uptake and transport of intact NPs was size-dependent, and in combination with the accumulation within the roots suggests a filtering effect of the plant cell walls at various points along the water transport pathway.

Original languageEnglish (US)
Pages (from-to)12537-12546
Number of pages10
JournalEnvironmental Science and Technology
Volume51
Issue number21
DOIs
StatePublished - Nov 7 2017

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Copper
Nanoparticles
copper
tomography
Positron emission tomography
Tomography
nanoparticle
Deodorants
Sun hoods
Food supply
Azides
Cycloaddition
Cosmetics
crop plant
lubricant
Electron transitions
electron microscopy
food supply
void
Electron microscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

In Vivo Tracking of Copper-64 Radiolabeled Nanoparticles in Lactuca sativa. / Davis, Ryan A.; Rippner, Devin A.; Hausner, Sven H.; Parikh, Sanjai J.; McElrone, Andrew J.; Sutcliffe, Julie.

In: Environmental Science and Technology, Vol. 51, No. 21, 07.11.2017, p. 12537-12546.

Research output: Contribution to journalArticle

Davis, Ryan A. ; Rippner, Devin A. ; Hausner, Sven H. ; Parikh, Sanjai J. ; McElrone, Andrew J. ; Sutcliffe, Julie. / In Vivo Tracking of Copper-64 Radiolabeled Nanoparticles in Lactuca sativa. In: Environmental Science and Technology. 2017 ; Vol. 51, No. 21. pp. 12537-12546.
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