In vivo, three-dimensional microscopy of trabecular bone

J. H. Kinney, Nancy E Lane, D. L. Haupt

Research output: Contribution to journalArticle

193 Citations (Scopus)

Abstract

Osteoporosis, which is correlated with low bone mass and increased bone fragility, is responsible for about 1.2 million fractures per year in the United States. We have used the three-dimensional (3-D), X-ray tomographic microscope to image the trabecular bone architecture of the proximal tibias of six Sprague-Dawley rats in vivo. Three of these rats were then ovariectomized to induce estrogen depletion, and three remained as controls. Five weeks later, the tibias were reimaged. The ovariectomized rats lost approximately 65% of their trabecular bone volume as compared with an insignificant change in the control rats. The connectivity density of the trabecular bone, as measured by the Euler characteristic, was linearly correlated with trabecular bone volume, even in the ovariectomized rats. Hypoestrogenemic bone loss manifested itself in greatly reduced connectivity and fewer trabecular elements, but not in thinning of trabeculae. The ability to microscopically image sequential changes in the 3-D architecture of trabecular bone in vivo will allow exploration of the earliest stages of hypoestrogenemic bone loss and to more rapidly test the effectiveness of new clinical treatments for this major public health problem.

Original languageEnglish (US)
Pages (from-to)264-270
Number of pages7
JournalJournal of Bone and Mineral Research
Volume10
Issue number2
StatePublished - 1995
Externally publishedYes

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Microscopy
Bone and Bones
Tibia
Osteoporosis
Sprague Dawley Rats
Estrogens
Public Health
X-Rays
Cancellous Bone

ASJC Scopus subject areas

  • Surgery

Cite this

In vivo, three-dimensional microscopy of trabecular bone. / Kinney, J. H.; Lane, Nancy E; Haupt, D. L.

In: Journal of Bone and Mineral Research, Vol. 10, No. 2, 1995, p. 264-270.

Research output: Contribution to journalArticle

Kinney, J. H. ; Lane, Nancy E ; Haupt, D. L. / In vivo, three-dimensional microscopy of trabecular bone. In: Journal of Bone and Mineral Research. 1995 ; Vol. 10, No. 2. pp. 264-270.
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