In vivo spectroscopic monitoring of renal ischemia and reperfusion in a rat model

Rajesh N. Raman, Christopher D. Pivetti, Dennis L Matthews, Christoph Troppmann, Stavros G. Demos

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Currently no clinical tool exists that measures the degree of ischemic injury incurred in tissue and assesses tissue function following transplantation. In response to this clinical problem, we explore optical spectroscopy to quantitatively assess ischemic injury. In our method we monitor the autofluorescence intensities under excitation suitable to excite specific tissue fluorophores. Specifically, a first excitation probes NADH, a biomolecule known to change its emission properties depending on the tissue's metabolic state. A second excitation is used to mainly probe tryptophan, a biomolecule expected to be minimally affected by metabolism. We postulate that the ratio of the two autofluorescence signals can be used to monitor tissue behavior during ischemia and reperfusion. To evaluate this approach, we acquire autofluorescence images of the injured and contralateral control kidney in vivo in a rat model under excitation at both wavelengths during injury and reperfusion. Our results indicate that this approach has the potential to provide real-time monitoring of organ function during transplantation.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6091
DOIs
StatePublished - 2006
EventOptical Biopsy VI - San Jose, CA, United States
Duration: Jan 24 2006Jan 24 2006

Other

OtherOptical Biopsy VI
CountryUnited States
CitySan Jose, CA
Period1/24/061/24/06

Keywords

  • In vivo
  • Ischemia
  • Kidney
  • Optical spectroscopy
  • Organ transplantation
  • Rat
  • Reperfusion
  • Tissue viability

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Raman, R. N., Pivetti, C. D., Matthews, D. L., Troppmann, C., & Demos, S. G. (2006). In vivo spectroscopic monitoring of renal ischemia and reperfusion in a rat model. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6091). [609103] https://doi.org/10.1117/12.645344