In vivo screening of artificial small RNAs for silencing endogenous genes in Escherichia coli

Vandana Sharma, Yohei Yokobayashi

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Bacterial noncoding small RNAs (sRNAs) modulate expression of numerous genes through antisense interactions with mRNAs. This chapter describes an in vivo screening strategy to engineer artificial sRNAs that can posttranscriptionally regulate desired endogenous genes in Escherichia coli. Artificial sRNA libraries are constructed by randomizing the antisense domain of natural sRNAs and screened for gene silencing activity using a cotransformed reporter vector. These small synthetic riboregulators can be used in synthetic gene circuits to control cell functions by directly targeting endogenous genes.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages75-84
Number of pages10
Volume1073
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1073
ISSN (Print)10643745

Keywords

  • Gene regulation
  • Gene silencing
  • Noncoding RNA
  • RNA engineering
  • Small RNA
  • Translational regulation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Sharma, V., & Yokobayashi, Y. (2013). In vivo screening of artificial small RNAs for silencing endogenous genes in Escherichia coli. In Methods in Molecular Biology (Vol. 1073, pp. 75-84). (Methods in Molecular Biology; Vol. 1073). https://doi.org/10.1007/978-1-62703-625-2_8