In vivo quantification of mouse autoimmune arthritis by PET/CT

Smriti Kundu-Raychaudhuri, Anupam Mitra, Ananya Datta-Mitra, Abhijit Chaudhari, Siba P Raychaudhuri

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Aim: To quantify the progression and severity of mouse collagen-induced arthritis (CIA) using an in vivo imaging tool, 18F-fluorodeoxyglucose (18F-FDG) PET/CT and validate it against gold standard 'histopathological' evaluation. Method: The PET radiotracer 18F-FDG, a marker for glucose metabolism, was injected in mice at different stages of CIA and the radiotracer distribution was imaged using a PET scanner. A sequential CT scan provided correlated anatomy. Radiotracer concentration was derived from PET/CT images for individual limb joints and on a per-limb basis at different stages of the disease. The imaging outcomes were subjected to correlation analysis with concurrently measured clinical and histological score. Results: Clinical and histological score, and hence disease severity, showed a strong linear correlation (r2 = 0.71, P = 0.001 and r2 = 0.87, P < 0.001, respectively) with radiotracer concentration measured from PET/CT during the progression of CIA. Conclusions: The strong positive correlation of the 18F-FDG PET/CT findings with the histopathological evaluation at different stages of the disease suggest the potential of this imaging tool for the non-invasive assessment of progression and severity in mouse autoimmune arthritis. Thus, in preclinical studies, 18F-FDG PET/CT can be considered as a non-invasive tool to develop novel therapies of inflammatory arthritis.

Original languageEnglish (US)
JournalInternational Journal of Rheumatic Diseases
DOIs
StateAccepted/In press - 2014

Keywords

  • in vivo imaging
  • Inflammation
  • Mouse CIA
  • PET/CT
  • Quantification

ASJC Scopus subject areas

  • Rheumatology

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