In vivo quantification of autofluorescence dynamics during renal ischemia and reperfusion under dual UV excitation

Rajesh N. Raman; Christopher D. Pivetti; Dennis L Matthews; Christoph Troppmann; Stavros G. Demos

doi:10.1117/12.699744

In vivo quantification of autofluorescence dynamics during renal ischemia and reperfusion under dual UV excitation

Rajesh N. Raman, Christopher D. Pivetti, Dennis L Matthews, Christoph Troppmann, Stavros G. Demos

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We explore an optical spectroscopy approach to monitor the progression of ischemia and reperfusion in situ using a rat model. The system utilizes the sensitivity of NADH emission to changes in cell metabolism during ischemia and reperfusion. In addition, the emission from tryptophan is employed as a normalization against changes in other optical properties of the tissue. Ischemia was induced in one kidney followed by at least 60 minutes of reperfusion. During both phases, autofluorescence images of the exposed surfaces of both the ischemic kidney and the normal (control) kidney were acquired and the respective average emission intensities were determined. Preliminary results indicate that the kinetics of the ratio of the emissions under these two excitations is related to the injury time.

Original language	English (US)
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6441
DOIs	https://doi.org/10.1117/12.699744
State	Published - 2007
Event	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V - San Jose, CA, United States Duration: Jan 22 2007 → Jan 24 2007

Other

Other	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Country	United States
City	San Jose, CA
Period	1/22/07 → 1/24/07

Keywords

Autofluorescence
In vivo
Ischemia
Kidney
Organ transplantation
Rat
Reperfusion
Tissue viability

ASJC Scopus subject areas

Engineering(all)

Access to Document

10.1117/12.699744

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Raman, R. N., Pivetti, C. D., Matthews, D. L., Troppmann, C., & Demos, S. G. (2007). In vivo quantification of autofluorescence dynamics during renal ischemia and reperfusion under dual UV excitation. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6441). [64410J] https://doi.org/10.1117/12.699744

In vivo quantification of autofluorescence dynamics during renal ischemia and reperfusion under dual UV excitation. / Raman, Rajesh N.; Pivetti, Christopher D.; Matthews, Dennis L ; Troppmann, Christoph; Demos, Stavros G.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6441 2007. 64410J.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Raman, RN, Pivetti, CD, Matthews, DL , Troppmann, C & Demos, SG 2007, In vivo quantification of autofluorescence dynamics during renal ischemia and reperfusion under dual UV excitation. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6441, 64410J, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, San Jose, CA, United States, 1/22/07. https://doi.org/10.1117/12.699744

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abstract = "We explore an optical spectroscopy approach to monitor the progression of ischemia and reperfusion in situ using a rat model. The system utilizes the sensitivity of NADH emission to changes in cell metabolism during ischemia and reperfusion. In addition, the emission from tryptophan is employed as a normalization against changes in other optical properties of the tissue. Ischemia was induced in one kidney followed by at least 60 minutes of reperfusion. During both phases, autofluorescence images of the exposed surfaces of both the ischemic kidney and the normal (control) kidney were acquired and the respective average emission intensities were determined. Preliminary results indicate that the kinetics of the ratio of the emissions under these two excitations is related to the injury time.",

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AB - We explore an optical spectroscopy approach to monitor the progression of ischemia and reperfusion in situ using a rat model. The system utilizes the sensitivity of NADH emission to changes in cell metabolism during ischemia and reperfusion. In addition, the emission from tryptophan is employed as a normalization against changes in other optical properties of the tissue. Ischemia was induced in one kidney followed by at least 60 minutes of reperfusion. During both phases, autofluorescence images of the exposed surfaces of both the ischemic kidney and the normal (control) kidney were acquired and the respective average emission intensities were determined. Preliminary results indicate that the kinetics of the ratio of the emissions under these two excitations is related to the injury time.

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KW - Ischemia

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KW - Organ transplantation

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KW - Reperfusion

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