Abstract
We explore an optical spectroscopy approach to monitor the progression of ischemia and reperfusion in situ using a rat model. The system utilizes the sensitivity of NADH emission to changes in cell metabolism during ischemia and reperfusion. In addition, the emission from tryptophan is employed as a normalization against changes in other optical properties of the tissue. Ischemia was induced in one kidney followed by at least 60 minutes of reperfusion. During both phases, autofluorescence images of the exposed surfaces of both the ischemic kidney and the normal (control) kidney were acquired and the respective average emission intensities were determined. Preliminary results indicate that the kinetics of the ratio of the emissions under these two excitations is related to the injury time.
Original language | English (US) |
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Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
Volume | 6441 |
DOIs | |
State | Published - 2007 |
Event | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V - San Jose, CA, United States Duration: Jan 22 2007 → Jan 24 2007 |
Other
Other | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V |
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Country | United States |
City | San Jose, CA |
Period | 1/22/07 → 1/24/07 |
Keywords
- Autofluorescence
- In vivo
- Ischemia
- Kidney
- Organ transplantation
- Rat
- Reperfusion
- Tissue viability
ASJC Scopus subject areas
- Engineering(all)