In vivo quantification of autofluorescence dynamics during renal ischemia and reperfusion under dual UV excitation

Rajesh N. Raman, Christopher D. Pivetti, Dennis L Matthews, Christoph Troppmann, Stavros G. Demos

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We explore an optical spectroscopy approach to monitor the progression of ischemia and reperfusion in situ using a rat model. The system utilizes the sensitivity of NADH emission to changes in cell metabolism during ischemia and reperfusion. In addition, the emission from tryptophan is employed as a normalization against changes in other optical properties of the tissue. Ischemia was induced in one kidney followed by at least 60 minutes of reperfusion. During both phases, autofluorescence images of the exposed surfaces of both the ischemic kidney and the normal (control) kidney were acquired and the respective average emission intensities were determined. Preliminary results indicate that the kinetics of the ratio of the emissions under these two excitations is related to the injury time.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6441
DOIs
StatePublished - 2007
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V - San Jose, CA, United States
Duration: Jan 22 2007Jan 24 2007

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
CountryUnited States
CitySan Jose, CA
Period1/22/071/24/07

Keywords

  • Autofluorescence
  • In vivo
  • Ischemia
  • Kidney
  • Organ transplantation
  • Rat
  • Reperfusion
  • Tissue viability

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Raman, R. N., Pivetti, C. D., Matthews, D. L., Troppmann, C., & Demos, S. G. (2007). In vivo quantification of autofluorescence dynamics during renal ischemia and reperfusion under dual UV excitation. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6441). [64410J] https://doi.org/10.1117/12.699744