Abstract
We explore an optical spectroscopy approach to monitor the progression of ischemia and reperfusion in situ using a rat model. The system utilizes the sensitivity of NADH emission to changes in cell metabolism during ischemia and reperfusion. In addition, the emission from tryptophan is employed as a normalization against changes in other optical properties of the tissue. Ischemia was induced in one kidney followed by at least 60 minutes of reperfusion. During both phases, autofluorescence images of the exposed surfaces of both the ischemic kidney and the normal (control) kidney were acquired and the respective average emission intensities were determined. Preliminary results indicate that the kinetics of the ratio of the emissions under these two excitations is related to the injury time.
| Original language | English (US) |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 6441 |
| DOIs | |
| State | Published - 2007 |
| Event | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V - San Jose, CA, United States Duration: Jan 22 2007 → Jan 24 2007 |
Other
| Other | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 1/22/07 → 1/24/07 |
Fingerprint
Keywords
- Autofluorescence
- In vivo
- Ischemia
- Kidney
- Organ transplantation
- Rat
- Reperfusion
- Tissue viability
ASJC Scopus subject areas
- Engineering(all)
Cite this
In vivo quantification of autofluorescence dynamics during renal ischemia and reperfusion under dual UV excitation. / Raman, Rajesh N.; Pivetti, Christopher D.; Matthews, Dennis L; Troppmann, Christoph; Demos, Stavros G.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6441 2007. 64410J.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - In vivo quantification of autofluorescence dynamics during renal ischemia and reperfusion under dual UV excitation
AU - Raman, Rajesh N.
AU - Pivetti, Christopher D.
AU - Matthews, Dennis L
AU - Troppmann, Christoph
AU - Demos, Stavros G.
PY - 2007
Y1 - 2007
N2 - We explore an optical spectroscopy approach to monitor the progression of ischemia and reperfusion in situ using a rat model. The system utilizes the sensitivity of NADH emission to changes in cell metabolism during ischemia and reperfusion. In addition, the emission from tryptophan is employed as a normalization against changes in other optical properties of the tissue. Ischemia was induced in one kidney followed by at least 60 minutes of reperfusion. During both phases, autofluorescence images of the exposed surfaces of both the ischemic kidney and the normal (control) kidney were acquired and the respective average emission intensities were determined. Preliminary results indicate that the kinetics of the ratio of the emissions under these two excitations is related to the injury time.
AB - We explore an optical spectroscopy approach to monitor the progression of ischemia and reperfusion in situ using a rat model. The system utilizes the sensitivity of NADH emission to changes in cell metabolism during ischemia and reperfusion. In addition, the emission from tryptophan is employed as a normalization against changes in other optical properties of the tissue. Ischemia was induced in one kidney followed by at least 60 minutes of reperfusion. During both phases, autofluorescence images of the exposed surfaces of both the ischemic kidney and the normal (control) kidney were acquired and the respective average emission intensities were determined. Preliminary results indicate that the kinetics of the ratio of the emissions under these two excitations is related to the injury time.
KW - Autofluorescence
KW - In vivo
KW - Ischemia
KW - Kidney
KW - Organ transplantation
KW - Rat
KW - Reperfusion
KW - Tissue viability
UR - http://www.scopus.com/inward/record.url?scp=34247366061&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34247366061&partnerID=8YFLogxK
U2 - 10.1117/12.699744
DO - 10.1117/12.699744
M3 - Conference contribution
AN - SCOPUS:34247366061
SN - 0819465542
SN - 9780819465542
VL - 6441
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ER -