In vivo ocular imaging of the cornea of the normal female laboratory beagle using confocal microscopy

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Objective: To obtain normative data for the normal laboratory beagle cornea using high-resolution in vivo confocal microscopy (IVCM). Animals studied: Sixteen eyes of eight healthy young female intact beagles. Procedures: The central cornea was imaged using IVCM. Mixed effects linear regression was used for statistical analysis. Results: in vivo confocal microscopy allowed detailed visualization and quantification of epithelial cells (superficial epithelial cell diameter: 43.25 ± 6.64 μm, basal cell diameter: 4.43 ± 0.67 μm), and nerve fibers (subepithelial nerve fiber diameter: 2.38 ± 0.69 μm, anterior stromal nerve fiber diameter: 16.93 ± 4.55 μm). Keratocyte density (anterior stroma 993.38 ± 134.24 cells/mm2, posterior stroma 789.38 ± 87.13 cells/mm2) and endothelial cell density (2815.18 ± 212.59 cells/mm2) were also measured. Conclusion: High-resolution IVCM provides detailed noninvasive evaluation of the cornea in the normal laboratory beagle.

Original languageEnglish (US)
Pages (from-to)63-67
Number of pages5
JournalVeterinary Ophthalmology
Issue number1
StatePublished - Jan 1 2016



  • Canine
  • Confocal microscopy
  • Cornea
  • Corneal nerves
  • Endothelial cell density
  • Laboratory beagle

ASJC Scopus subject areas

  • veterinary(all)

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