Abstract
Our early studies showed that tissue diffusion coefficient (Dt) of small solute sodium fluorescein (0.45 nm radius) in frog mesentery is 30% of its free diffusion coefficient (Dfree) in aqueous solution. We test here the hypothesis that because of its size-limiting structure the interstitium would provide larger resistance to larger solute α-lactalbumin (2.0 nm radius). We extended our previous method by using laser-scanning confocal microscopy to measure both solute capillary permeability (P) and solute tissue diffusion coefficients (Dt) from the rate of tissue solute accumulation and the radial concentration gradients around individually perfused microvessels in frog mesentery. Pα-lactalbumin was 1.7 ± 0.7 (SD) × 10-6 cm/s (n = 6). Dt/Dfree was 27% ± 5% (SD) (n = 6). This value is comparable to that for small solute sodium fluorescein. Our results indicate that frog mesenteric interstitium is less selective to larger solute α-lactalbumin than the microvessel wall.
| Original language | English (US) |
|---|---|
| Title of host publication | American Society of Mechanical Engineers, Bioengineering Division (Publication) BED |
| Place of Publication | Fairfield, NJ, United States |
| Publisher | ASME |
| Pages | 167-174 |
| Number of pages | 8 |
| Volume | 44 |
| ISBN (Print) | 0791816435 |
| State | Published - 1999 |
| Event | Advances in Heat and Mass Transfer in Biotechnology - 1999 (The ASME International Mechanical Engineering Congress and Exposition) - Nashville, TN, USA Duration: Nov 14 1999 → Nov 19 1999 |
Other
| Other | Advances in Heat and Mass Transfer in Biotechnology - 1999 (The ASME International Mechanical Engineering Congress and Exposition) |
|---|---|
| City | Nashville, TN, USA |
| Period | 11/14/99 → 11/19/99 |
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ASJC Scopus subject areas
- Engineering(all)
Cite this
In vivo measurement of microvessel permeability and tissue diffusion coefficient in frog mesentery by confocal microscopy. / Fu, Bingmei M.; Adamson, Roger H.; Curry, Fitz Roy E.
American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. Vol. 44 Fairfield, NJ, United States : ASME, 1999. p. 167-174.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - In vivo measurement of microvessel permeability and tissue diffusion coefficient in frog mesentery by confocal microscopy
AU - Fu, Bingmei M.
AU - Adamson, Roger H.
AU - Curry, Fitz Roy E
PY - 1999
Y1 - 1999
N2 - Our early studies showed that tissue diffusion coefficient (Dt) of small solute sodium fluorescein (0.45 nm radius) in frog mesentery is 30% of its free diffusion coefficient (Dfree) in aqueous solution. We test here the hypothesis that because of its size-limiting structure the interstitium would provide larger resistance to larger solute α-lactalbumin (2.0 nm radius). We extended our previous method by using laser-scanning confocal microscopy to measure both solute capillary permeability (P) and solute tissue diffusion coefficients (Dt) from the rate of tissue solute accumulation and the radial concentration gradients around individually perfused microvessels in frog mesentery. Pα-lactalbumin was 1.7 ± 0.7 (SD) × 10-6 cm/s (n = 6). Dt/Dfree was 27% ± 5% (SD) (n = 6). This value is comparable to that for small solute sodium fluorescein. Our results indicate that frog mesenteric interstitium is less selective to larger solute α-lactalbumin than the microvessel wall.
AB - Our early studies showed that tissue diffusion coefficient (Dt) of small solute sodium fluorescein (0.45 nm radius) in frog mesentery is 30% of its free diffusion coefficient (Dfree) in aqueous solution. We test here the hypothesis that because of its size-limiting structure the interstitium would provide larger resistance to larger solute α-lactalbumin (2.0 nm radius). We extended our previous method by using laser-scanning confocal microscopy to measure both solute capillary permeability (P) and solute tissue diffusion coefficients (Dt) from the rate of tissue solute accumulation and the radial concentration gradients around individually perfused microvessels in frog mesentery. Pα-lactalbumin was 1.7 ± 0.7 (SD) × 10-6 cm/s (n = 6). Dt/Dfree was 27% ± 5% (SD) (n = 6). This value is comparable to that for small solute sodium fluorescein. Our results indicate that frog mesenteric interstitium is less selective to larger solute α-lactalbumin than the microvessel wall.
UR - http://www.scopus.com/inward/record.url?scp=0033295534&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033295534&partnerID=8YFLogxK
M3 - Chapter
SN - 0791816435
VL - 44
SP - 167
EP - 174
BT - American Society of Mechanical Engineers, Bioengineering Division (Publication) BED
PB - ASME
CY - Fairfield, NJ, United States
ER -