In vivo measurement of microvessel permeability and tissue diffusion coefficient in frog mesentery by confocal microscopy

Bingmei M. Fu, Roger H. Adamson, Fitz Roy E Curry

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Our early studies showed that tissue diffusion coefficient (Dt) of small solute sodium fluorescein (0.45 nm radius) in frog mesentery is 30% of its free diffusion coefficient (Dfree) in aqueous solution. We test here the hypothesis that because of its size-limiting structure the interstitium would provide larger resistance to larger solute α-lactalbumin (2.0 nm radius). We extended our previous method by using laser-scanning confocal microscopy to measure both solute capillary permeability (P) and solute tissue diffusion coefficients (Dt) from the rate of tissue solute accumulation and the radial concentration gradients around individually perfused microvessels in frog mesentery. Pα-lactalbumin was 1.7 ± 0.7 (SD) × 10-6 cm/s (n = 6). Dt/Dfree was 27% ± 5% (SD) (n = 6). This value is comparable to that for small solute sodium fluorescein. Our results indicate that frog mesenteric interstitium is less selective to larger solute α-lactalbumin than the microvessel wall.

Original languageEnglish (US)
Title of host publicationAmerican Society of Mechanical Engineers, Bioengineering Division (Publication) BED
Place of PublicationFairfield, NJ, United States
PublisherASME
Pages167-174
Number of pages8
Volume44
ISBN (Print)0791816435
StatePublished - 1999
EventAdvances in Heat and Mass Transfer in Biotechnology - 1999 (The ASME International Mechanical Engineering Congress and Exposition) - Nashville, TN, USA
Duration: Nov 14 1999Nov 19 1999

Other

OtherAdvances in Heat and Mass Transfer in Biotechnology - 1999 (The ASME International Mechanical Engineering Congress and Exposition)
CityNashville, TN, USA
Period11/14/9911/19/99

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ASJC Scopus subject areas

  • Engineering(all)

Cite this

Fu, B. M., Adamson, R. H., & Curry, F. R. E. (1999). In vivo measurement of microvessel permeability and tissue diffusion coefficient in frog mesentery by confocal microscopy. In American Society of Mechanical Engineers, Bioengineering Division (Publication) BED (Vol. 44, pp. 167-174). ASME.