In vivo measurement of microvessel permeability and tissue diffusion coefficient in frog mesentery by confocal microscopy

Bingmei M. Fu; Roger H. Adamson; Fitz Roy E Curry

In vivo measurement of microvessel permeability and tissue diffusion coefficient in frog mesentery by confocal microscopy

Bingmei M. Fu, Roger H. Adamson, Fitz Roy E Curry

Physiology and Membrane Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

Our early studies showed that tissue diffusion coefficient (D_t) of small solute sodium fluorescein (0.45 nm radius) in frog mesentery is 30% of its free diffusion coefficient (D_free) in aqueous solution. We test here the hypothesis that because of its size-limiting structure the interstitium would provide larger resistance to larger solute α-lactalbumin (2.0 nm radius). We extended our previous method by using laser-scanning confocal microscopy to measure both solute capillary permeability (P) and solute tissue diffusion coefficients (D_t) from the rate of tissue solute accumulation and the radial concentration gradients around individually perfused microvessels in frog mesentery. P^{α-lactalbumin} was 1.7 ± 0.7 (SD) × 10^-6 cm/s (n = 6). D_t/D_free was 27% ± 5% (SD) (n = 6). This value is comparable to that for small solute sodium fluorescein. Our results indicate that frog mesenteric interstitium is less selective to larger solute α-lactalbumin than the microvessel wall.

Original language	English (US)
Title of host publication	American Society of Mechanical Engineers, Bioengineering Division (Publication) BED
Place of Publication	Fairfield, NJ, United States
Publisher	ASME
Pages	167-174
Number of pages	8
Volume	44
ISBN (Print)	0791816435
State	Published - 1999
Event	Advances in Heat and Mass Transfer in Biotechnology - 1999 (The ASME International Mechanical Engineering Congress and Exposition) - Nashville, TN, USA Duration: Nov 14 1999 → Nov 19 1999

Other

Other	Advances in Heat and Mass Transfer in Biotechnology - 1999 (The ASME International Mechanical Engineering Congress and Exposition)
City	Nashville, TN, USA
Period	11/14/99 → 11/19/99

ASJC Scopus subject areas

Engineering(all)

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In vivo measurement of microvessel permeability and tissue diffusion coefficient in frog mesentery by confocal microscopy. / Fu, Bingmei M.; Adamson, Roger H.; Curry, Fitz Roy E.

American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. Vol. 44 Fairfield, NJ, United States : ASME, 1999. p. 167-174.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Fu, BM, Adamson, RH & Curry, FRE 1999, In vivo measurement of microvessel permeability and tissue diffusion coefficient in frog mesentery by confocal microscopy. in American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. vol. 44, ASME, Fairfield, NJ, United States, pp. 167-174, Advances in Heat and Mass Transfer in Biotechnology - 1999 (The ASME International Mechanical Engineering Congress and Exposition), Nashville, TN, USA, 11/14/99.

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abstract = "Our early studies showed that tissue diffusion coefficient (Dt) of small solute sodium fluorescein (0.45 nm radius) in frog mesentery is 30% of its free diffusion coefficient (Dfree) in aqueous solution. We test here the hypothesis that because of its size-limiting structure the interstitium would provide larger resistance to larger solute α-lactalbumin (2.0 nm radius). We extended our previous method by using laser-scanning confocal microscopy to measure both solute capillary permeability (P) and solute tissue diffusion coefficients (Dt) from the rate of tissue solute accumulation and the radial concentration gradients around individually perfused microvessels in frog mesentery. Pα-lactalbumin was 1.7 ± 0.7 (SD) × 10-6 cm/s (n = 6). Dt/Dfree was 27% ± 5% (SD) (n = 6). This value is comparable to that for small solute sodium fluorescein. Our results indicate that frog mesenteric interstitium is less selective to larger solute α-lactalbumin than the microvessel wall.",

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N2 - Our early studies showed that tissue diffusion coefficient (Dt) of small solute sodium fluorescein (0.45 nm radius) in frog mesentery is 30% of its free diffusion coefficient (Dfree) in aqueous solution. We test here the hypothesis that because of its size-limiting structure the interstitium would provide larger resistance to larger solute α-lactalbumin (2.0 nm radius). We extended our previous method by using laser-scanning confocal microscopy to measure both solute capillary permeability (P) and solute tissue diffusion coefficients (Dt) from the rate of tissue solute accumulation and the radial concentration gradients around individually perfused microvessels in frog mesentery. Pα-lactalbumin was 1.7 ± 0.7 (SD) × 10-6 cm/s (n = 6). Dt/Dfree was 27% ± 5% (SD) (n = 6). This value is comparable to that for small solute sodium fluorescein. Our results indicate that frog mesenteric interstitium is less selective to larger solute α-lactalbumin than the microvessel wall.

AB - Our early studies showed that tissue diffusion coefficient (Dt) of small solute sodium fluorescein (0.45 nm radius) in frog mesentery is 30% of its free diffusion coefficient (Dfree) in aqueous solution. We test here the hypothesis that because of its size-limiting structure the interstitium would provide larger resistance to larger solute α-lactalbumin (2.0 nm radius). We extended our previous method by using laser-scanning confocal microscopy to measure both solute capillary permeability (P) and solute tissue diffusion coefficients (Dt) from the rate of tissue solute accumulation and the radial concentration gradients around individually perfused microvessels in frog mesentery. Pα-lactalbumin was 1.7 ± 0.7 (SD) × 10-6 cm/s (n = 6). Dt/Dfree was 27% ± 5% (SD) (n = 6). This value is comparable to that for small solute sodium fluorescein. Our results indicate that frog mesenteric interstitium is less selective to larger solute α-lactalbumin than the microvessel wall.

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