In vivo label-free structural and biochemical imaging of coronary arteries using an integrated ultrasound and multispectral fluorescence lifetime catheter system

Julien Bec, Jennifer E. Phipps, Dimitris Gorpas, Dinglong Ma, Hussain Fatakdawala, Kenneth B. Margulies, Jeffrey Southard, Laura Marcu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Existing clinical intravascular imaging modalities are not capable of accurate detection of critical plaque pathophysiology in the coronary arteries. This study reports the first intravascular catheter combining intravascular ultrasound (IVUS) with multispectral fluorescence lifetime imaging (FLIm) that enables label-free simultaneous assessment of morphological and biochemical features of coronary vessels in vivo. A 3.7 Fr catheter with a fiber-optic channel was constructed based on a 40 MHz clinical IVUS catheter. The ability to safely acquire co-registered FLIm-IVUS data in vivo using Dextran40 solution flushing was demonstrated in swine coronary arteries. FLIm parameters from the arterial wall were consistent with the emission of fluorophores present in healthy arterial wall (collagen, elastin). Additionally, structural and biochemical features from atherosclerotic lesions were acquired in ex vivo human coronary samples and corroborated with histological findings. Current results show that FLIm parameters linked to the amount of structural proteins (e.g. collagen, elastin) and lipids (e.g. foam cells, extracellular lipids) in the first 200 μm of the intima provide important biochemical information that can supplement IVUS data for a comprehensive assessment of plaques pathophysiology. The unique FLIm-IVUS system evaluated here has the potential to provide a comprehensive insight into atherosclerotic lesion formation, diagnostics and response to therapy.

Original languageEnglish (US)
Article number8960
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Optical Imaging
Coronary Vessels
Catheters
Fluorescence
Elastin
Collagen
Lipids
Foam Cells
Swine
Proteins

ASJC Scopus subject areas

  • General

Cite this

In vivo label-free structural and biochemical imaging of coronary arteries using an integrated ultrasound and multispectral fluorescence lifetime catheter system. / Bec, Julien; Phipps, Jennifer E.; Gorpas, Dimitris; Ma, Dinglong; Fatakdawala, Hussain; Margulies, Kenneth B.; Southard, Jeffrey; Marcu, Laura.

In: Scientific Reports, Vol. 7, No. 1, 8960, 01.12.2017.

Research output: Contribution to journalArticle

Bec, Julien ; Phipps, Jennifer E. ; Gorpas, Dimitris ; Ma, Dinglong ; Fatakdawala, Hussain ; Margulies, Kenneth B. ; Southard, Jeffrey ; Marcu, Laura. / In vivo label-free structural and biochemical imaging of coronary arteries using an integrated ultrasound and multispectral fluorescence lifetime catheter system. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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