In vivo imaging reveals transient microglia recruitment and functional recovery of photoreceptor signaling after injury

Eric B. Miller, Pengfei Zhang, Karli Ching, Edward N Pugh Jr, Marie E Burns

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Microglia respond to damage and microenvironmental changes within the central nervous system by morphologically transforming and migrating to the lesion, but the real-time behavior of populations of these resident immune cells and the neurons they support have seldom been observed simultaneously. Here, we have used in vivo high-resolution optical coherence tomography (OCT) and scanning laser ophthalmoscopy with and without adaptive optics to quantify the 3D distribution and dynamics of microglia in the living retina before and after local damage to photoreceptors. Following photoreceptor injury, microglia migrated both laterally and vertically through the retina over many hours, forming a tight cluster within the area of visible damage that resolved over 2 wk. In vivo OCT optophysiological assessment revealed that the photoreceptors occupying the damaged region lost all light-driven signaling during the period of microglia recruitment. Remarkably, photoreceptors recovered function to near-baseline levels after the microglia had departed the injury locus. These results demonstrate the spatiotemporal dynamics of microglia engagement and restoration of neuronal function during tissue remodeling and highlight the need for mechanistic studies that consider the temporal and structural dynamics of neuron-microglia interactions in vivo.

Original languageEnglish (US)
Pages (from-to)16603-16612
Number of pages10
JournalProceedings of the National Academy of Sciences of the United States of America
Volume116
Issue number33
DOIs
StatePublished - Jan 1 2019

Keywords

  • Imaging
  • Laser
  • Microglia
  • Photoreceptor
  • Retina

ASJC Scopus subject areas

  • General

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