In vivo imaging of neuronal activation and plasticity in the rat brain by high resolution positron emission tomography (microPET)

Harley I. Kornblum, Dalia M. Araujo, Alexander J. Annala, Keith J. Tatsukawa, Michael E. Phelps, Simon R Cherry

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

The study of neural repair and neuroplasticity in rodents would be enhanced by the ability to assess neuronal function in vivo. Positron emission tomography (PET) is used to study brain plasticity in humans, but the limited resolution and sensitivity of conventional scanners have generally precluded the use of PET to study neuroplasticity in rodents. We now demonstrate that microPET, a PET scanner developed for use with small animals, can be used to assess metabolic activity in different regions of the conscious rodent brain using [18F]fluorodeoxyglucose (FDG) as the tracer, and to monitor changes in neuronal activity. Limbic seizures result in dramatically elevated metabolic activity in the hippocampus, whereas vibrissal stimulation results in more modest increases in FDG uptake in the contralateral neocortex. We also show that microPET can be used to study lesion-induced plasticity of the brain. Cerebral hemidecortication resulted in diminished relative glucose metabolism in the neostriatum and thalamus ipsilateral to the lesion, with subsequent, significant recovery of metabolic function. These studies demonstrate that microPET can be used for serial assessment of metabolic function of individual, awake rats with a minimal degree of invasiveness, and therefore, has the potential for use in the study of brain disorders and repair.

Original languageEnglish (US)
Pages (from-to)655-660
Number of pages6
JournalNature Biotechnology
Volume18
Issue number6
DOIs
StatePublished - Jun 2000

Fingerprint

Positron emission tomography
Neuronal Plasticity
Positron-Emission Tomography
Plasticity
Rats
Rodentia
Brain
Chemical activation
Imaging techniques
Neostriatum
Repair
Neocortex
Recovery of Function
Fluorodeoxyglucose F18
Brain Diseases
Thalamus
Hippocampus
Seizures
Metabolism
Glucose

Keywords

  • Brain
  • Glucose metabolism
  • Hemispherectomy
  • Seizure
  • Vibrissal stimulation

ASJC Scopus subject areas

  • Microbiology

Cite this

In vivo imaging of neuronal activation and plasticity in the rat brain by high resolution positron emission tomography (microPET). / Kornblum, Harley I.; Araujo, Dalia M.; Annala, Alexander J.; Tatsukawa, Keith J.; Phelps, Michael E.; Cherry, Simon R.

In: Nature Biotechnology, Vol. 18, No. 6, 06.2000, p. 655-660.

Research output: Contribution to journalArticle

Kornblum, Harley I. ; Araujo, Dalia M. ; Annala, Alexander J. ; Tatsukawa, Keith J. ; Phelps, Michael E. ; Cherry, Simon R. / In vivo imaging of neuronal activation and plasticity in the rat brain by high resolution positron emission tomography (microPET). In: Nature Biotechnology. 2000 ; Vol. 18, No. 6. pp. 655-660.
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