In vivo functional imaging of intrinsic scattering changes in the human retina with high-speed ultrahigh resolution OCT

Vivek Srinivasan, Y. Chen, J. S. Duker, J. G. Fujimoto

Research output: Contribution to journalArticle

56 Scopus citations


Non-invasive methods of probing retinal function are of interest for the early detection of retinal disease. While retinal function is traditionally directly measured with the electroretinogram (ERG), recently functional optical imaging of the retina has been demonstrated. In this manuscript, stimulus-induced, intrinsic optical scattering changes in the human retina are measured in vivo with high-speed, ultrahigh resolution optical coherence tomography (OCT) operating at 50,000 axial scans per second and ∼3.3 micron axial resolution. A stimulus and measurement protocol that enables measurement of functional OCT retinal signals is described. OCT signal changes in the photoreceptors are demonstrated. Two distinct responses having different temporal and spatial properties are reported. These results are discussed in the context of optical intrinsic signals measured previously in the retina by fundus imaging and scanning laser ophthalmoscopy. Finally, challenges associated with in vivo functional retinal imaging in human subjects are discussed.

Original languageEnglish (US)
Pages (from-to)3861-3877
Number of pages17
JournalOptics Express
Issue number5
StatePublished - Mar 2 2009
Externally publishedYes


ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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