In vivo expression of helicobacter pylori virulence genes in patients with gastritis, ulcer, and gastric cancer

Francisco Avilés-Jiménez, Adriana Reyes-Leon, Erik Nieto-Patlán, Lori M. Hansen, Juan Burgueño, Irma P. Ramos, Margarita Camorlinga-Ponce, Hector Bermúdez, Juan M. Blancas, Lourdes Cabrera, Rosa María Ribas-Aparicio, Jay V Solnick, Javier Torres-López

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The best-studied Helicobacter pylori virulence factor associated with development of peptic ulcer disease or gastric cancer (GC) rather than asymptomatic nonatrophic gastritis (NAG) is the cag pathogenicity island (cagPAI), which encodes a type IV secretion system (T4SS) that injects the CagA oncoprotein into host epithelial cells. Here we used real-time reverse transcription-PCR (RT-PCR) to measure the in vivo expression of genes on the cagPAI and of other virulence genes in patients with NAG, duodenal ulcer (DU), or GC. In vivo expression of H. pylori virulence genes was greater overall in gastric biopsy specimens of patients with GC than in those of patients with NAG or DU. However, since in vitro expression of cagA was not greater in H. pylori strains from patients with GC than in those from patients with NAG or DU, increased expression in GC in vivo is likely a result of environmental conditions in the gastric mucosa, though it may in turn cause more severe pathology. Increased expression of virulence genes in GC may represent a stress response to elevated pH or other environmental conditions in the stomach of patients with GC, which may be less hospitable to H. pylori colonization than the acidic environment in patients with NAG or DU.

Original languageEnglish (US)
Pages (from-to)594-601
Number of pages8
JournalInfection and Immunity
Volume80
Issue number2
DOIs
StatePublished - Feb 2012

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

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    Avilés-Jiménez, F., Reyes-Leon, A., Nieto-Patlán, E., Hansen, L. M., Burgueño, J., Ramos, I. P., Camorlinga-Ponce, M., Bermúdez, H., Blancas, J. M., Cabrera, L., Ribas-Aparicio, R. M., Solnick, J. V., & Torres-López, J. (2012). In vivo expression of helicobacter pylori virulence genes in patients with gastritis, ulcer, and gastric cancer. Infection and Immunity, 80(2), 594-601. https://doi.org/10.1128/IAI.05845-11