In vivo exposure to ozone depletes vitamins C and E and induces lipid peroxidation in epidermal layers of murine skin

Jens J. Thiele, Maret G. Traber, Kenneth Tsang, Carroll E Cross, Lester Packer

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

To evaluate skin susceptibility to ozone (O3) and to localize possible oxidative damage within the skin layer, hairless mice were exposed to 10 ppm O3 or air (0 ppm O3) for 2 h. The mice were euthanized, the skin removed and frozen. Three skin layers (upper epidermis, lower epidermis/papillary dermis, and dermis) were separated, antioxidant concentrations (α-tocopherol and ascorbic acid) and the lipid peroxidation product malondialdehyde (MDA) measured. In the upper epidermis, O3 significantly depleted α-tocopherol (22%; p < .05) and ascorbic acid (55%; p < .01). These antioxidants were unchanged by O3 in the lower skin layers. More remarkably, MDA increased ten-fold in the upper epidermis (p < .001) and two-fold in the lower epidermis/papillary epidermis (p < .05): it was unchanged in the dermis. Thus, exposure to O3 in vivo depletes ascorbic acid and α-tocopherol and strongly induces lipid peroxidation in skin. High MDA concentrations measured in the upper epidermis suggest that O3 reacts directly with fatty acids on the skin surface layers. These results further suggest that chronic exposure to lower O3 concentrations found in urban smog could potentially have implications for skin health.

Original languageEnglish (US)
Pages (from-to)385-391
Number of pages7
JournalFree Radical Biology and Medicine
Volume23
Issue number3
DOIs
StatePublished - 1997

Fingerprint

Ozone
Vitamin E
Lipid Peroxidation
Ascorbic Acid
Epidermis
Skin
Lipids
Tocopherols
Dermis
Malondialdehyde
Smog
Antioxidants
Hairless Mouse
Fatty Acids
Air
Health

Keywords

  • α-Tocopherol
  • Ascorbic acid
  • Free radicals
  • Malondialdehyde
  • Oxidative stress
  • Skin

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

In vivo exposure to ozone depletes vitamins C and E and induces lipid peroxidation in epidermal layers of murine skin. / Thiele, Jens J.; Traber, Maret G.; Tsang, Kenneth; Cross, Carroll E; Packer, Lester.

In: Free Radical Biology and Medicine, Vol. 23, No. 3, 1997, p. 385-391.

Research output: Contribution to journalArticle

Thiele, Jens J. ; Traber, Maret G. ; Tsang, Kenneth ; Cross, Carroll E ; Packer, Lester. / In vivo exposure to ozone depletes vitamins C and E and induces lipid peroxidation in epidermal layers of murine skin. In: Free Radical Biology and Medicine. 1997 ; Vol. 23, No. 3. pp. 385-391.
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