In vivo evaluation of the cornea and conjunctiva of the normal laboratory beagle using time- and Fourier-domain optical coherence tomography and ultrasound pachymetry

Ann R. Strom, Dennis E. Cortés, Carol A. Rasmussen, Sara M Thomasy, Kim Mcintyre, Shwu Fei Lee, Philip H Kass, Mark J Mannis, Christopher J Murphy

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Objective: To obtain normative data for the canine cornea and conjunctiva using high-resolution time- and Fourier-domain optical coherence tomography (TD-OCT and FD-OCT) and ultrasound pachymetry (USP). Animals: One hundred sixty-eight eyes of 133 healthy young intact laboratory beagles. Procedures: The cornea and conjunctiva of 16 eyes of 8 healthy young intact female beagles were imaged using FD-OCT. Corneal thickness was measured with FD-OCT and USP, while corneal epithelial thickness and conjunctival epithelial thickness were measured with FD-OCT. The central corneal thickness (CCT) was determined in 152 eyes of 125 healthy young adult intact female (35) and male (90) beagles using TD-OCT. Mixed effects linear regression was used for statistical analysis. Results: The CCT was (mean ± standard deviation) 497.54 ± 29.76, 555.49 ± 17.19, and 594.81 ± 33.02 μm as measured by FD-OCT, USP, and TD-OCT, respectively. The central, superior paraxial, superior perilimbal corneal epithelial thickness and superior bulbar conjunctival epithelial thickness were 52.38 ± 7.27, 56.96 ± 6.47, 69.06 ± 8.84 and 42.98 ± 6.17 μm, respectively. When comparing techniques used for measuring CCT (USP vs. FD-OCT and FD-OCT vs. TD-OCT), USP and TD-OCT generated significantly greater values in comparison with FD-OCT (both P <0.001). For all dogs, CCT increased with increasing age and body weight (both P <0.001) and was higher in intact males vs. females using TD-OCT (P = 0.034). Conclusion: High-resolution FD-OCT and TD-OCT provide detailed noninvasive evaluation of in vivo canine anterior segment structures. Normative values of the canine cornea and conjunctiva are reported.

Original languageEnglish (US)
Pages (from-to)50-56
Number of pages7
JournalVeterinary Ophthalmology
Volume19
Issue number1
DOIs
StatePublished - Jan 1 2016

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Keywords

  • Canine
  • Conjunctiva
  • Cornea
  • Laboratory beagle
  • Optical coherence tomography
  • Ultrasound pachymetry

ASJC Scopus subject areas

  • veterinary(all)

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