In vivo evaluation of the cornea and conjunctiva of the normal laboratory beagle using time- and Fourier-domain optical coherence tomography and ultrasound pachymetry

Ann R. Strom, Dennis E. Cortés, Carol A. Rasmussen, Sara M Thomasy, Kim Mcintyre, Shwu Fei Lee, Philip H Kass, Mark J Mannis, Christopher J Murphy

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objective: To obtain normative data for the canine cornea and conjunctiva using high-resolution time- and Fourier-domain optical coherence tomography (TD-OCT and FD-OCT) and ultrasound pachymetry (USP). Animals: One hundred sixty-eight eyes of 133 healthy young intact laboratory beagles. Procedures: The cornea and conjunctiva of 16 eyes of 8 healthy young intact female beagles were imaged using FD-OCT. Corneal thickness was measured with FD-OCT and USP, while corneal epithelial thickness and conjunctival epithelial thickness were measured with FD-OCT. The central corneal thickness (CCT) was determined in 152 eyes of 125 healthy young adult intact female (35) and male (90) beagles using TD-OCT. Mixed effects linear regression was used for statistical analysis. Results: The CCT was (mean ± standard deviation) 497.54 ± 29.76, 555.49 ± 17.19, and 594.81 ± 33.02 μm as measured by FD-OCT, USP, and TD-OCT, respectively. The central, superior paraxial, superior perilimbal corneal epithelial thickness and superior bulbar conjunctival epithelial thickness were 52.38 ± 7.27, 56.96 ± 6.47, 69.06 ± 8.84 and 42.98 ± 6.17 μm, respectively. When comparing techniques used for measuring CCT (USP vs. FD-OCT and FD-OCT vs. TD-OCT), USP and TD-OCT generated significantly greater values in comparison with FD-OCT (both P <0.001). For all dogs, CCT increased with increasing age and body weight (both P <0.001) and was higher in intact males vs. females using TD-OCT (P = 0.034). Conclusion: High-resolution FD-OCT and TD-OCT provide detailed noninvasive evaluation of in vivo canine anterior segment structures. Normative values of the canine cornea and conjunctiva are reported.

Original languageEnglish (US)
Pages (from-to)50-56
Number of pages7
JournalVeterinary Ophthalmology
Volume19
Issue number1
DOIs
StatePublished - Jan 1 2016

Fingerprint

conjunctiva
Conjunctiva
tomography
Optical Coherence Tomography
cornea
Beagle
Cornea
Canidae
Corneal Pachymetry
Young Adult
Linear Models
dogs
eyes
Body Weight
Dogs
young adults
statistical analysis
body weight

Keywords

  • Canine
  • Conjunctiva
  • Cornea
  • Laboratory beagle
  • Optical coherence tomography
  • Ultrasound pachymetry

ASJC Scopus subject areas

  • veterinary(all)

Cite this

In vivo evaluation of the cornea and conjunctiva of the normal laboratory beagle using time- and Fourier-domain optical coherence tomography and ultrasound pachymetry. / Strom, Ann R.; Cortés, Dennis E.; Rasmussen, Carol A.; Thomasy, Sara M; Mcintyre, Kim; Lee, Shwu Fei; Kass, Philip H; Mannis, Mark J; Murphy, Christopher J.

In: Veterinary Ophthalmology, Vol. 19, No. 1, 01.01.2016, p. 50-56.

Research output: Contribution to journalArticle

@article{fd3fe1880d9e4cbbbaed6721f25b5a94,
title = "In vivo evaluation of the cornea and conjunctiva of the normal laboratory beagle using time- and Fourier-domain optical coherence tomography and ultrasound pachymetry",
abstract = "Objective: To obtain normative data for the canine cornea and conjunctiva using high-resolution time- and Fourier-domain optical coherence tomography (TD-OCT and FD-OCT) and ultrasound pachymetry (USP). Animals: One hundred sixty-eight eyes of 133 healthy young intact laboratory beagles. Procedures: The cornea and conjunctiva of 16 eyes of 8 healthy young intact female beagles were imaged using FD-OCT. Corneal thickness was measured with FD-OCT and USP, while corneal epithelial thickness and conjunctival epithelial thickness were measured with FD-OCT. The central corneal thickness (CCT) was determined in 152 eyes of 125 healthy young adult intact female (35) and male (90) beagles using TD-OCT. Mixed effects linear regression was used for statistical analysis. Results: The CCT was (mean ± standard deviation) 497.54 ± 29.76, 555.49 ± 17.19, and 594.81 ± 33.02 μm as measured by FD-OCT, USP, and TD-OCT, respectively. The central, superior paraxial, superior perilimbal corneal epithelial thickness and superior bulbar conjunctival epithelial thickness were 52.38 ± 7.27, 56.96 ± 6.47, 69.06 ± 8.84 and 42.98 ± 6.17 μm, respectively. When comparing techniques used for measuring CCT (USP vs. FD-OCT and FD-OCT vs. TD-OCT), USP and TD-OCT generated significantly greater values in comparison with FD-OCT (both P <0.001). For all dogs, CCT increased with increasing age and body weight (both P <0.001) and was higher in intact males vs. females using TD-OCT (P = 0.034). Conclusion: High-resolution FD-OCT and TD-OCT provide detailed noninvasive evaluation of in vivo canine anterior segment structures. Normative values of the canine cornea and conjunctiva are reported.",
keywords = "Canine, Conjunctiva, Cornea, Laboratory beagle, Optical coherence tomography, Ultrasound pachymetry",
author = "Strom, {Ann R.} and Cort{\'e}s, {Dennis E.} and Rasmussen, {Carol A.} and Thomasy, {Sara M} and Kim Mcintyre and Lee, {Shwu Fei} and Kass, {Philip H} and Mannis, {Mark J} and Murphy, {Christopher J}",
year = "2016",
month = "1",
day = "1",
doi = "10.1111/vop.12256",
language = "English (US)",
volume = "19",
pages = "50--56",
journal = "Veterinary Ophthalmology",
issn = "1463-5216",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - In vivo evaluation of the cornea and conjunctiva of the normal laboratory beagle using time- and Fourier-domain optical coherence tomography and ultrasound pachymetry

AU - Strom, Ann R.

AU - Cortés, Dennis E.

AU - Rasmussen, Carol A.

AU - Thomasy, Sara M

AU - Mcintyre, Kim

AU - Lee, Shwu Fei

AU - Kass, Philip H

AU - Mannis, Mark J

AU - Murphy, Christopher J

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Objective: To obtain normative data for the canine cornea and conjunctiva using high-resolution time- and Fourier-domain optical coherence tomography (TD-OCT and FD-OCT) and ultrasound pachymetry (USP). Animals: One hundred sixty-eight eyes of 133 healthy young intact laboratory beagles. Procedures: The cornea and conjunctiva of 16 eyes of 8 healthy young intact female beagles were imaged using FD-OCT. Corneal thickness was measured with FD-OCT and USP, while corneal epithelial thickness and conjunctival epithelial thickness were measured with FD-OCT. The central corneal thickness (CCT) was determined in 152 eyes of 125 healthy young adult intact female (35) and male (90) beagles using TD-OCT. Mixed effects linear regression was used for statistical analysis. Results: The CCT was (mean ± standard deviation) 497.54 ± 29.76, 555.49 ± 17.19, and 594.81 ± 33.02 μm as measured by FD-OCT, USP, and TD-OCT, respectively. The central, superior paraxial, superior perilimbal corneal epithelial thickness and superior bulbar conjunctival epithelial thickness were 52.38 ± 7.27, 56.96 ± 6.47, 69.06 ± 8.84 and 42.98 ± 6.17 μm, respectively. When comparing techniques used for measuring CCT (USP vs. FD-OCT and FD-OCT vs. TD-OCT), USP and TD-OCT generated significantly greater values in comparison with FD-OCT (both P <0.001). For all dogs, CCT increased with increasing age and body weight (both P <0.001) and was higher in intact males vs. females using TD-OCT (P = 0.034). Conclusion: High-resolution FD-OCT and TD-OCT provide detailed noninvasive evaluation of in vivo canine anterior segment structures. Normative values of the canine cornea and conjunctiva are reported.

AB - Objective: To obtain normative data for the canine cornea and conjunctiva using high-resolution time- and Fourier-domain optical coherence tomography (TD-OCT and FD-OCT) and ultrasound pachymetry (USP). Animals: One hundred sixty-eight eyes of 133 healthy young intact laboratory beagles. Procedures: The cornea and conjunctiva of 16 eyes of 8 healthy young intact female beagles were imaged using FD-OCT. Corneal thickness was measured with FD-OCT and USP, while corneal epithelial thickness and conjunctival epithelial thickness were measured with FD-OCT. The central corneal thickness (CCT) was determined in 152 eyes of 125 healthy young adult intact female (35) and male (90) beagles using TD-OCT. Mixed effects linear regression was used for statistical analysis. Results: The CCT was (mean ± standard deviation) 497.54 ± 29.76, 555.49 ± 17.19, and 594.81 ± 33.02 μm as measured by FD-OCT, USP, and TD-OCT, respectively. The central, superior paraxial, superior perilimbal corneal epithelial thickness and superior bulbar conjunctival epithelial thickness were 52.38 ± 7.27, 56.96 ± 6.47, 69.06 ± 8.84 and 42.98 ± 6.17 μm, respectively. When comparing techniques used for measuring CCT (USP vs. FD-OCT and FD-OCT vs. TD-OCT), USP and TD-OCT generated significantly greater values in comparison with FD-OCT (both P <0.001). For all dogs, CCT increased with increasing age and body weight (both P <0.001) and was higher in intact males vs. females using TD-OCT (P = 0.034). Conclusion: High-resolution FD-OCT and TD-OCT provide detailed noninvasive evaluation of in vivo canine anterior segment structures. Normative values of the canine cornea and conjunctiva are reported.

KW - Canine

KW - Conjunctiva

KW - Cornea

KW - Laboratory beagle

KW - Optical coherence tomography

KW - Ultrasound pachymetry

UR - http://www.scopus.com/inward/record.url?scp=84953635289&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84953635289&partnerID=8YFLogxK

U2 - 10.1111/vop.12256

DO - 10.1111/vop.12256

M3 - Article

C2 - 25676065

AN - SCOPUS:84953635289

VL - 19

SP - 50

EP - 56

JO - Veterinary Ophthalmology

JF - Veterinary Ophthalmology

SN - 1463-5216

IS - 1

ER -