In vivo cytokine response to experimental feline infectious peritonitis virus infection

Gregg A. Dean, Thierry Olivry, Christine Stanton, Niels C Pedersen

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Feline infectious peritonitis virus (FIPV) is a coronavirus that causes sporadic fatal disease in cats characterized by vasculitis, granulomatous inflammation and effusive pleuritis/peritonitis. Histologic changes in lymphoid tissues include lymphoid hyperplasia, lymphoid depletion, histiocytosis, and granuloma formation. Although viremia occurs, histologic lesions are not found uniformly throughout lymphoid tissues. We used experimental infection of cats with a highly pathogenic FIPV isolate, UCD8, to study histologic lesions, virus replication, and cytokine expression in multiple lymphoid tissues during the effusive phase of disease. Viral RNA was found in 76% of central tissues (mediastinal lymph node, spleen, mesenteric lymph node) examined, as compared to 27% of peripheral tissues (popliteal lymph node, cervical lymph node, femoral bone marrow). All tissues positive for virus replication also demonstrated lymphoid depletion. Generally, affected tissues had lower levels of IL-4 and IL-12-p40 mRNA and higher levels of IL-10 mRNA. Although no differences in IFN-γ or TNF-α mRNA were measured, TNF-α protein expression was greater in affected tissues and demonstrated a shift in the source of TNF-α from macrophages to lymphocytes. Together, these results colocalize FIPV replication, lymphocyte depletion in tissues, and alterations in cytokine transcription and translation. A possible role for TNF-α in the previously described FIPV-induced lymphocyte apoptosis is also suggested.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalVeterinary Microbiology
Volume97
Issue number1-2
DOIs
StatePublished - Dec 2 2003

Fingerprint

Feline Coronavirus
Feline coronavirus
Virus Diseases
cytokines
Cytokines
Lymphoid Tissue
Virus Replication
Lymph Nodes
infection
lymph nodes
virus replication
Messenger RNA
lymphocytes
Cat Diseases
Lymphocytes
Histiocytosis
Lymphocyte Depletion
lesions (animal)
Pleurisy
Coronavirus

Keywords

  • Coronavirus
  • Cytokines
  • Feline infectious peritonitis
  • IL-10
  • TNF-α

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

In vivo cytokine response to experimental feline infectious peritonitis virus infection. / Dean, Gregg A.; Olivry, Thierry; Stanton, Christine; Pedersen, Niels C.

In: Veterinary Microbiology, Vol. 97, No. 1-2, 02.12.2003, p. 1-12.

Research output: Contribution to journalArticle

Dean, Gregg A. ; Olivry, Thierry ; Stanton, Christine ; Pedersen, Niels C. / In vivo cytokine response to experimental feline infectious peritonitis virus infection. In: Veterinary Microbiology. 2003 ; Vol. 97, No. 1-2. pp. 1-12.
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