In Vivo CRISPR Screen Identifies TgWIP as a Toxoplasma Modulator of Dendritic Cell Migration

Lamba Omar Sangaré, Einar B. Ólafsson, Yifan Wang, Ninghan Yang, Lindsay Julien, Ana Camejo, Patricia Pesavento, Saima M. Sidik, Sebastian Lourido, Antonio Barragan, Jeroen P.J. Saeij

Research output: Contribution to journalArticle

Abstract

Sangaré et al. use a CRISPR screen to determine the in vivo fitness of Toxoplasma genes. They identify a secreted protein, TgWIP, which determines dendritic cell motility and transmigration in the infected host. In mice, TgWIP determines T. gondii dissemination from the site of infection to distant organs.

Original languageEnglish (US)
Pages (from-to)478-492.e8
JournalCell Host and Microbe
Volume26
Issue number4
DOIs
StatePublished - Oct 9 2019

Fingerprint

Clustered Regularly Interspaced Short Palindromic Repeats
Toxoplasma
Dendritic Cells
Cell Movement
Infection
Genes
Proteins

Keywords

  • actin
  • CRISPR
  • cyst
  • dendritic cell motility
  • dissemination
  • loss-of-function screen
  • migration
  • Toxoplasma gondii
  • virulence
  • WAVE complex

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

Cite this

In Vivo CRISPR Screen Identifies TgWIP as a Toxoplasma Modulator of Dendritic Cell Migration. / Sangaré, Lamba Omar; Ólafsson, Einar B.; Wang, Yifan; Yang, Ninghan; Julien, Lindsay; Camejo, Ana; Pesavento, Patricia; Sidik, Saima M.; Lourido, Sebastian; Barragan, Antonio; Saeij, Jeroen P.J.

In: Cell Host and Microbe, Vol. 26, No. 4, 09.10.2019, p. 478-492.e8.

Research output: Contribution to journalArticle

Sangaré, LO, Ólafsson, EB, Wang, Y, Yang, N, Julien, L, Camejo, A, Pesavento, P, Sidik, SM, Lourido, S, Barragan, A & Saeij, JPJ 2019, 'In Vivo CRISPR Screen Identifies TgWIP as a Toxoplasma Modulator of Dendritic Cell Migration', Cell Host and Microbe, vol. 26, no. 4, pp. 478-492.e8. https://doi.org/10.1016/j.chom.2019.09.008
Sangaré, Lamba Omar ; Ólafsson, Einar B. ; Wang, Yifan ; Yang, Ninghan ; Julien, Lindsay ; Camejo, Ana ; Pesavento, Patricia ; Sidik, Saima M. ; Lourido, Sebastian ; Barragan, Antonio ; Saeij, Jeroen P.J. / In Vivo CRISPR Screen Identifies TgWIP as a Toxoplasma Modulator of Dendritic Cell Migration. In: Cell Host and Microbe. 2019 ; Vol. 26, No. 4. pp. 478-492.e8.
@article{83d03c761550475980e2a64d147d1b61,
title = "In Vivo CRISPR Screen Identifies TgWIP as a Toxoplasma Modulator of Dendritic Cell Migration",
abstract = "Sangar{\'e} et al. use a CRISPR screen to determine the in vivo fitness of Toxoplasma genes. They identify a secreted protein, TgWIP, which determines dendritic cell motility and transmigration in the infected host. In mice, TgWIP determines T. gondii dissemination from the site of infection to distant organs.",
keywords = "actin, CRISPR, cyst, dendritic cell motility, dissemination, loss-of-function screen, migration, Toxoplasma gondii, virulence, WAVE complex",
author = "Sangar{\'e}, {Lamba Omar} and {\'O}lafsson, {Einar B.} and Yifan Wang and Ninghan Yang and Lindsay Julien and Ana Camejo and Patricia Pesavento and Sidik, {Saima M.} and Sebastian Lourido and Antonio Barragan and Saeij, {Jeroen P.J.}",
year = "2019",
month = "10",
day = "9",
doi = "10.1016/j.chom.2019.09.008",
language = "English (US)",
volume = "26",
pages = "478--492.e8",
journal = "Cell Host and Microbe",
issn = "1931-3128",
publisher = "Cell Press",
number = "4",

}

TY - JOUR

T1 - In Vivo CRISPR Screen Identifies TgWIP as a Toxoplasma Modulator of Dendritic Cell Migration

AU - Sangaré, Lamba Omar

AU - Ólafsson, Einar B.

AU - Wang, Yifan

AU - Yang, Ninghan

AU - Julien, Lindsay

AU - Camejo, Ana

AU - Pesavento, Patricia

AU - Sidik, Saima M.

AU - Lourido, Sebastian

AU - Barragan, Antonio

AU - Saeij, Jeroen P.J.

PY - 2019/10/9

Y1 - 2019/10/9

N2 - Sangaré et al. use a CRISPR screen to determine the in vivo fitness of Toxoplasma genes. They identify a secreted protein, TgWIP, which determines dendritic cell motility and transmigration in the infected host. In mice, TgWIP determines T. gondii dissemination from the site of infection to distant organs.

AB - Sangaré et al. use a CRISPR screen to determine the in vivo fitness of Toxoplasma genes. They identify a secreted protein, TgWIP, which determines dendritic cell motility and transmigration in the infected host. In mice, TgWIP determines T. gondii dissemination from the site of infection to distant organs.

KW - actin

KW - CRISPR

KW - cyst

KW - dendritic cell motility

KW - dissemination

KW - loss-of-function screen

KW - migration

KW - Toxoplasma gondii

KW - virulence

KW - WAVE complex

UR - http://www.scopus.com/inward/record.url?scp=85072626172&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85072626172&partnerID=8YFLogxK

U2 - 10.1016/j.chom.2019.09.008

DO - 10.1016/j.chom.2019.09.008

M3 - Article

C2 - 31600500

AN - SCOPUS:85072626172

VL - 26

SP - 478-492.e8

JO - Cell Host and Microbe

JF - Cell Host and Microbe

SN - 1931-3128

IS - 4

ER -