In Vivo CRISPR Screen Identifies TgWIP as a Toxoplasma Modulator of Dendritic Cell Migration

Lamba Omar Sangaré, Einar B. Ólafsson, Yifan Wang, Ninghan Yang, Lindsay Julien, Ana Camejo, Patricia Pesavento, Saima M. Sidik, Sebastian Lourido, Antonio Barragan, Jeroen P.J. Saeij

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Sangaré et al. use a CRISPR screen to determine the in vivo fitness of Toxoplasma genes. They identify a secreted protein, TgWIP, which determines dendritic cell motility and transmigration in the infected host. In mice, TgWIP determines T. gondii dissemination from the site of infection to distant organs.

Original languageEnglish (US)
Pages (from-to)478-492.e8
JournalCell Host and Microbe
Volume26
Issue number4
DOIs
StatePublished - Oct 9 2019

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Keywords

  • actin
  • CRISPR
  • cyst
  • dendritic cell motility
  • dissemination
  • loss-of-function screen
  • migration
  • Toxoplasma gondii
  • virulence
  • WAVE complex

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

Cite this

Sangaré, L. O., Ólafsson, E. B., Wang, Y., Yang, N., Julien, L., Camejo, A., Pesavento, P., Sidik, S. M., Lourido, S., Barragan, A., & Saeij, J. P. J. (2019). In Vivo CRISPR Screen Identifies TgWIP as a Toxoplasma Modulator of Dendritic Cell Migration. Cell Host and Microbe, 26(4), 478-492.e8. https://doi.org/10.1016/j.chom.2019.09.008