In vitro zinc stimulation of angiotensin-converting enzyme activities in various tissues of zinc-deficient rats

T. Tamura, L. E. Freeberg, K. E. Johnston, Carl L Keen

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Angiotensin-converting enzyme (ACE) activities and the effect of the in vitro addition of zinc on ACE activities were studied in various tissues obtained from weanling Sprague-Dawley male rats fed diets containing either 7.7 (zinc-deficient) or 770 μmol of zinc/kg of diet (control) ad libitum for 21 days. Tissue zinc concentrations were measured by atomic-absorption-spectrophotometric method and ACE activities by radioassay using [3H]hippuryl-glycyl-glycine as the substrate. The stimulation of ACE by the in vitro addition of zinc was measured using zinc at a concentration of 17 μmol/L. Plasma ACE activities in zinc-deficient rats were significantly lower than in controls, while ACE activities in lung were two times higher in zinc-deficient rats than in controls. ACE activities in testis were similar in zinc-deficient and control rats. The in vitro addition of zinc increased plasma ACE activities by 70% in zinc-deficient rats, while it had no effect in controls. In lung, testis and aorta, ACE activities were increased significantly by the in vitro addition of zinc in both zinc-deficient and control rats. Plasma ACE stimulation by the in vitro addition of zinc, a measure of apoenzyme production, may be used to evaluate zinc nutriture.

Original languageEnglish (US)
Pages (from-to)919-928
Number of pages10
JournalNutrition Research
Volume14
Issue number6
DOIs
StatePublished - 1994

Keywords

  • Angiotensin-converting enzyme
  • Rat tissues 01
  • Zinc deficiency
  • Zinc stimulation

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

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