Abstract
Angiotensin-converting enzyme (ACE) activities and the effect of the in vitro addition of zinc on ACE activities were studied in various tissues obtained from weanling Sprague-Dawley male rats fed diets containing either 7.7 (zinc-deficient) or 770 μmol of zinc/kg of diet (control) ad libitum for 21 days. Tissue zinc concentrations were measured by atomic-absorption-spectrophotometric method and ACE activities by radioassay using [3H]hippuryl-glycyl-glycine as the substrate. The stimulation of ACE by the in vitro addition of zinc was measured using zinc at a concentration of 17 μmol/L. Plasma ACE activities in zinc-deficient rats were significantly lower than in controls, while ACE activities in lung were two times higher in zinc-deficient rats than in controls. ACE activities in testis were similar in zinc-deficient and control rats. The in vitro addition of zinc increased plasma ACE activities by 70% in zinc-deficient rats, while it had no effect in controls. In lung, testis and aorta, ACE activities were increased significantly by the in vitro addition of zinc in both zinc-deficient and control rats. Plasma ACE stimulation by the in vitro addition of zinc, a measure of apoenzyme production, may be used to evaluate zinc nutriture.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 919-928 |
| Number of pages | 10 |
| Journal | Nutrition Research |
| Volume | 14 |
| Issue number | 6 |
| DOIs | |
| State | Published - 1994 |
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Keywords
- Angiotensin-converting enzyme
- Rat tissues 01
- Zinc deficiency
- Zinc stimulation
ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism
- Nutrition and Dietetics
Cite this
In vitro zinc stimulation of angiotensin-converting enzyme activities in various tissues of zinc-deficient rats. / Tamura, T.; Freeberg, L. E.; Johnston, K. E.; Keen, Carl L.
In: Nutrition Research, Vol. 14, No. 6, 1994, p. 919-928.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro zinc stimulation of angiotensin-converting enzyme activities in various tissues of zinc-deficient rats
AU - Tamura, T.
AU - Freeberg, L. E.
AU - Johnston, K. E.
AU - Keen, Carl L
PY - 1994
Y1 - 1994
N2 - Angiotensin-converting enzyme (ACE) activities and the effect of the in vitro addition of zinc on ACE activities were studied in various tissues obtained from weanling Sprague-Dawley male rats fed diets containing either 7.7 (zinc-deficient) or 770 μmol of zinc/kg of diet (control) ad libitum for 21 days. Tissue zinc concentrations were measured by atomic-absorption-spectrophotometric method and ACE activities by radioassay using [3H]hippuryl-glycyl-glycine as the substrate. The stimulation of ACE by the in vitro addition of zinc was measured using zinc at a concentration of 17 μmol/L. Plasma ACE activities in zinc-deficient rats were significantly lower than in controls, while ACE activities in lung were two times higher in zinc-deficient rats than in controls. ACE activities in testis were similar in zinc-deficient and control rats. The in vitro addition of zinc increased plasma ACE activities by 70% in zinc-deficient rats, while it had no effect in controls. In lung, testis and aorta, ACE activities were increased significantly by the in vitro addition of zinc in both zinc-deficient and control rats. Plasma ACE stimulation by the in vitro addition of zinc, a measure of apoenzyme production, may be used to evaluate zinc nutriture.
AB - Angiotensin-converting enzyme (ACE) activities and the effect of the in vitro addition of zinc on ACE activities were studied in various tissues obtained from weanling Sprague-Dawley male rats fed diets containing either 7.7 (zinc-deficient) or 770 μmol of zinc/kg of diet (control) ad libitum for 21 days. Tissue zinc concentrations were measured by atomic-absorption-spectrophotometric method and ACE activities by radioassay using [3H]hippuryl-glycyl-glycine as the substrate. The stimulation of ACE by the in vitro addition of zinc was measured using zinc at a concentration of 17 μmol/L. Plasma ACE activities in zinc-deficient rats were significantly lower than in controls, while ACE activities in lung were two times higher in zinc-deficient rats than in controls. ACE activities in testis were similar in zinc-deficient and control rats. The in vitro addition of zinc increased plasma ACE activities by 70% in zinc-deficient rats, while it had no effect in controls. In lung, testis and aorta, ACE activities were increased significantly by the in vitro addition of zinc in both zinc-deficient and control rats. Plasma ACE stimulation by the in vitro addition of zinc, a measure of apoenzyme production, may be used to evaluate zinc nutriture.
KW - Angiotensin-converting enzyme
KW - Rat tissues 01
KW - Zinc deficiency
KW - Zinc stimulation
UR - http://www.scopus.com/inward/record.url?scp=0028356586&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028356586&partnerID=8YFLogxK
U2 - 10.1016/S0271-5317(05)80492-0
DO - 10.1016/S0271-5317(05)80492-0
M3 - Article
AN - SCOPUS:0028356586
VL - 14
SP - 919
EP - 928
JO - Nutrition Research
JF - Nutrition Research
SN - 0271-5317
IS - 6
ER -