Abstract
Angiotensin-converting enzyme (ACE) activities and the effect of the in vitro addition of zinc on ACE activities were studied in various tissues obtained from weanling Sprague-Dawley male rats fed diets containing either 7.7 (zinc-deficient) or 770 μmol of zinc/kg of diet (control) ad libitum for 21 days. Tissue zinc concentrations were measured by atomic-absorption-spectrophotometric method and ACE activities by radioassay using [3H]hippuryl-glycyl-glycine as the substrate. The stimulation of ACE by the in vitro addition of zinc was measured using zinc at a concentration of 17 μmol/L. Plasma ACE activities in zinc-deficient rats were significantly lower than in controls, while ACE activities in lung were two times higher in zinc-deficient rats than in controls. ACE activities in testis were similar in zinc-deficient and control rats. The in vitro addition of zinc increased plasma ACE activities by 70% in zinc-deficient rats, while it had no effect in controls. In lung, testis and aorta, ACE activities were increased significantly by the in vitro addition of zinc in both zinc-deficient and control rats. Plasma ACE stimulation by the in vitro addition of zinc, a measure of apoenzyme production, may be used to evaluate zinc nutriture.
Original language | English (US) |
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Pages (from-to) | 919-928 |
Number of pages | 10 |
Journal | Nutrition Research |
Volume | 14 |
Issue number | 6 |
DOIs | |
State | Published - 1994 |
Keywords
- Angiotensin-converting enzyme
- Rat tissues 01
- Zinc deficiency
- Zinc stimulation
ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism
- Nutrition and Dietetics