In vitro platelet release by rat megakaryocytes: effect of metabolic inhibitors and cytoskeletal disrupting agents.

P. J. Handagama, B. F. Feldman, N. C. Jain, T. B. Farver, C. S. Kono

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Development of an in vitro visual assay facilitated the study of large numbers of megakaryocytes undergoing proplatelet formation in short-term cultures. Approximately 9% of megakaryocytes formed platelets during a 24-hour period. In the presence of an inhibitor of anaerobic glycolysis (NaF), proplatelet formation was inhibited, whereas inhibitors of respiration (NaCN) did not significantly (P greater than 0.05) decrease proplatelet formation. Presence of the microtubule-disrupting agents colchicine and vincristine sulfate in culture medium inhibited proplatelet formation, whereas the microfilament-disrupting agent cytochalasin B had a less pronounced inhibition.

Original languageEnglish (US)
Pages (from-to)1142-1146
Number of pages5
JournalAmerican Journal of Veterinary Research
Volume48
Issue number7
StatePublished - Jul 1987

ASJC Scopus subject areas

  • veterinary(all)

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