In vitro platelet release by rat megakaryocytes

effect of heterologous antiplatelet serum.

P. J. Handagama, N. C. Jain, B. F. Feldman, Thomas B Farver, C. S. Kono

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A visual assay to study megakaryocyte platelet release via proplatelet formation in vitro was established. Samples of megakaryocyte-enriched rat bone marrow were incubated (37 C) in RPMI-1640 medium with 15% autologous serum in specially prepared chambers. In the culture system, approximately 6% of megakaryocytes formed proplatelet processes within 24 hours. Inclusion of a heterologous antiplatelet antibody in the culture system inhibited proplatelet formation, compared with that in controls.

Original languageEnglish (US)
Pages (from-to)1147-1149
Number of pages3
JournalAmerican Journal of Veterinary Research
Volume48
Issue number7
StatePublished - Jul 1987

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megakaryocytes
Megakaryocytes
Blood Platelets
rats
Serum
Heterophile Antibodies
bone marrow
Bone Marrow
antibodies
assays
In Vitro Techniques
sampling

ASJC Scopus subject areas

  • veterinary(all)

Cite this

In vitro platelet release by rat megakaryocytes : effect of heterologous antiplatelet serum. / Handagama, P. J.; Jain, N. C.; Feldman, B. F.; Farver, Thomas B; Kono, C. S.

In: American Journal of Veterinary Research, Vol. 48, No. 7, 07.1987, p. 1147-1149.

Research output: Contribution to journalArticle

Handagama, P. J. ; Jain, N. C. ; Feldman, B. F. ; Farver, Thomas B ; Kono, C. S. / In vitro platelet release by rat megakaryocytes : effect of heterologous antiplatelet serum. In: American Journal of Veterinary Research. 1987 ; Vol. 48, No. 7. pp. 1147-1149.
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