In vitro platelet release by rat megakaryocytes: effect of heterologous antiplatelet serum.

P. J. Handagama, N. C. Jain, B. F. Feldman, T. B. Farver, C. S. Kono

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

A visual assay to study megakaryocyte platelet release via proplatelet formation in vitro was established. Samples of megakaryocyte-enriched rat bone marrow were incubated (37 C) in RPMI-1640 medium with 15% autologous serum in specially prepared chambers. In the culture system, approximately 6% of megakaryocytes formed proplatelet processes within 24 hours. Inclusion of a heterologous antiplatelet antibody in the culture system inhibited proplatelet formation, compared with that in controls.

Original languageEnglish (US)
Pages (from-to)1147-1149
Number of pages3
JournalAmerican Journal of Veterinary Research
Volume48
Issue number7
StatePublished - Jul 1987

ASJC Scopus subject areas

  • veterinary(all)

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