Abstract
A visual assay to study megakaryocyte platelet release via proplatelet formation in vitro was established. Samples of megakaryocyte-enriched rat bone marrow were incubated (37 C) in RPMI-1640 medium with 15% autologous serum in specially prepared chambers. In the culture system, approximately 6% of megakaryocytes formed proplatelet processes within 24 hours. Inclusion of a heterologous antiplatelet antibody in the culture system inhibited proplatelet formation, compared with that in controls.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1147-1149 |
| Number of pages | 3 |
| Journal | American Journal of Veterinary Research |
| Volume | 48 |
| Issue number | 7 |
| State | Published - Jul 1987 |
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ASJC Scopus subject areas
- veterinary(all)
Cite this
In vitro platelet release by rat megakaryocytes : effect of heterologous antiplatelet serum. / Handagama, P. J.; Jain, N. C.; Feldman, B. F.; Farver, T. B.; Kono, C. S.
In: American Journal of Veterinary Research, Vol. 48, No. 7, 07.1987, p. 1147-1149.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro platelet release by rat megakaryocytes
T2 - effect of heterologous antiplatelet serum.
AU - Handagama, P. J.
AU - Jain, N. C.
AU - Feldman, B. F.
AU - Farver, T. B.
AU - Kono, C. S.
PY - 1987/7
Y1 - 1987/7
N2 - A visual assay to study megakaryocyte platelet release via proplatelet formation in vitro was established. Samples of megakaryocyte-enriched rat bone marrow were incubated (37 C) in RPMI-1640 medium with 15% autologous serum in specially prepared chambers. In the culture system, approximately 6% of megakaryocytes formed proplatelet processes within 24 hours. Inclusion of a heterologous antiplatelet antibody in the culture system inhibited proplatelet formation, compared with that in controls.
AB - A visual assay to study megakaryocyte platelet release via proplatelet formation in vitro was established. Samples of megakaryocyte-enriched rat bone marrow were incubated (37 C) in RPMI-1640 medium with 15% autologous serum in specially prepared chambers. In the culture system, approximately 6% of megakaryocytes formed proplatelet processes within 24 hours. Inclusion of a heterologous antiplatelet antibody in the culture system inhibited proplatelet formation, compared with that in controls.
UR - http://www.scopus.com/inward/record.url?scp=0023371357&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023371357&partnerID=8YFLogxK
M3 - Article
C2 - 3631702
AN - SCOPUS:0023371357
VL - 48
SP - 1147
EP - 1149
JO - American Journal of Veterinary Research
JF - American Journal of Veterinary Research
SN - 0002-9645
IS - 7
ER -