Abstract
A visual assay to study megakaryocyte platelet release via proplatelet formation in vitro was established. Samples of megakaryocyte-enriched rat bone marrow were incubated (37 C) in RPMI-1640 medium with 15% autologous serum in specially prepared chambers. In the culture system, approximately 6% of megakaryocytes formed proplatelet processes within 24 hours. Inclusion of a heterologous antiplatelet antibody in the culture system inhibited proplatelet formation, compared with that in controls.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1147-1149 |
| Number of pages | 3 |
| Journal | American Journal of Veterinary Research |
| Volume | 48 |
| Issue number | 7 |
| State | Published - Jul 1987 |
ASJC Scopus subject areas
- veterinary(all)
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Cite this
Handagama, P. J., Jain, N. C., Feldman, B. F., Farver, T. B., & Kono, C. S. (1987). In vitro platelet release by rat megakaryocytes: effect of heterologous antiplatelet serum. American Journal of Veterinary Research, 48(7), 1147-1149.