In vitro platelet release by rat megakaryocytes: effect of heterologous antiplatelet serum.

P. J. Handagama; N. C. Jain; B. F. Feldman; T. B. Farver; C. S. Kono

In vitro platelet release by rat megakaryocytes: effect of heterologous antiplatelet serum.

P. J. Handagama, N. C. Jain, B. F. Feldman, T. B. Farver, C. S. Kono

School of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

A visual assay to study megakaryocyte platelet release via proplatelet formation in vitro was established. Samples of megakaryocyte-enriched rat bone marrow were incubated (37 C) in RPMI-1640 medium with 15% autologous serum in specially prepared chambers. In the culture system, approximately 6% of megakaryocytes formed proplatelet processes within 24 hours. Inclusion of a heterologous antiplatelet antibody in the culture system inhibited proplatelet formation, compared with that in controls.

Original language	English (US)
Pages (from-to)	1147-1149
Number of pages	3
Journal	American Journal of Veterinary Research
Volume	48
Issue number	7
State	Published - Jul 1987

ASJC Scopus subject areas

veterinary(all)

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abstract = "A visual assay to study megakaryocyte platelet release via proplatelet formation in vitro was established. Samples of megakaryocyte-enriched rat bone marrow were incubated (37 C) in RPMI-1640 medium with 15% autologous serum in specially prepared chambers. In the culture system, approximately 6% of megakaryocytes formed proplatelet processes within 24 hours. Inclusion of a heterologous antiplatelet antibody in the culture system inhibited proplatelet formation, compared with that in controls.",

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AU - Handagama, P. J.

AU - Jain, N. C.

AU - Feldman, B. F.

AU - Farver, T. B.

AU - Kono, C. S.

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AB - A visual assay to study megakaryocyte platelet release via proplatelet formation in vitro was established. Samples of megakaryocyte-enriched rat bone marrow were incubated (37 C) in RPMI-1640 medium with 15% autologous serum in specially prepared chambers. In the culture system, approximately 6% of megakaryocytes formed proplatelet processes within 24 hours. Inclusion of a heterologous antiplatelet antibody in the culture system inhibited proplatelet formation, compared with that in controls.

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In vitro platelet release by rat megakaryocytes: effect of heterologous antiplatelet serum.

Abstract

ASJC Scopus subject areas

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