In vitro macrophage tropism of pathogenic and nonpathogenic molecular clones of simian immunodeficiency virus (SIVmac)

Babak Banapour, Marta Marthas, Robert J. Munn, Paul A Luciw

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40 Citations (Scopus)

Abstract

The significance of infection of mononuclear phagocytes by immunodeficiency lentiviruses of primates is not clearly established. To explore the relationship of macrophage tropism and pathogenesis, conditions to culture and infect monocyte-derived macrophages from rhesus macaques were established and the growth properties of two molecular proviral clones of simian immunodeficiency virus (SIVmac) were studied. Rhesus macrophages supported productive infection of the nonpathogenic SIVmac-1A11 clone; extensive cytopathology characterized by formation of multinucleated giant cells and release of particle-associated reverse transcriptase activity in culture supernatant were observed. In contrast, the pathogenic SIVmac-239 did not establish a productive infection of macrophages and no cytopathology was observed. Both SIVmac-1A11 and SIVmac-239 replicated and induced cytopathic effects in cultures of rhesus peripheral blood lymphocytes and the Cemx174 lymphoid cell line. These results, together with the previously published reports on the pathogenic potential of these two clones of SIVmac, suggest that macrophage tropism measured in vitro does not correlate with in vitro does not correlate with in vivo virulence.

Original languageEnglish (US)
Pages (from-to)12-19
Number of pages8
JournalVirology
Volume183
Issue number1
DOIs
StatePublished - 1991

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Simian Immunodeficiency Virus
Tropism
Clone Cells
Macrophages
Primate Lentiviruses
Infection
Lymphocytes
RNA-Directed DNA Polymerase
Giant Cells
Phagocytes
Macaca mulatta
Virulence
In Vitro Techniques
Cell Line
Growth

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

In vitro macrophage tropism of pathogenic and nonpathogenic molecular clones of simian immunodeficiency virus (SIVmac). / Banapour, Babak; Marthas, Marta; Munn, Robert J.; Luciw, Paul A.

In: Virology, Vol. 183, No. 1, 1991, p. 12-19.

Research output: Contribution to journalArticle

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