In vitro isolation and characterization of a Neospora sp. from aborted bovine foetuses

Patricia A Conrad, B. C. Barr, K. W. Sverlow, Mark L Anderson, B. Daft, Hailu Kinde, J. P. Dubey, L. Munson, A. Ardans

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

A Neospora sp. was isolated from the brains of two aborted bovine foetuses and grown continuously in vitro in bovine cell cultures. A comparison of the antigenic reactivity of in vitro cultivated tachyzoites with polyclonal antisera to Neospora caninum, Hammondia hammondi or Toxoplasma gondii revealed that the bovine protozoal isolates were similar to N. caninum and antigenically distinct from T. gondii. Tachyzoites of both bovine isolates had similar ultrastructural features, including an apical polar ring, conoid, electron-dense rhoptries and micronemes. The orientation of the micronemes, presence of micropores and a large number of electron-dense granules in the posterior portion of the bovine isolate tachyzoites differed from previous descriptions of N. caninum in vivo. Tachyzoites of the bovine isolates were ultrastructurally more similar to in vitro cultivated N. caninum tachyzoites than to tachyzoites of T. gondii or H. hammondi. The antigenic and ultrastructural similarities between N. caninum and the protozoal parasites isolated from aborted bovine foetuses in this study support the proposition that these parasites belong to the genus Neospora.

Original languageEnglish (US)
Pages (from-to)239-249
Number of pages11
JournalParasitology
Volume106
Issue number3
StatePublished - 1993

Fingerprint

Aborted Fetus
Neospora
tachyzoites
fetus
Neospora caninum
cattle
Toxoplasma
Hammondia hammondi
Toxoplasma gondii
Parasites
electrons
Electrons
parasites
micropores
In Vitro Techniques
antiserum
Immune Sera
granules
cell culture
Cell Culture Techniques

ASJC Scopus subject areas

  • Immunology
  • Parasitology

Cite this

In vitro isolation and characterization of a Neospora sp. from aborted bovine foetuses. / Conrad, Patricia A; Barr, B. C.; Sverlow, K. W.; Anderson, Mark L; Daft, B.; Kinde, Hailu; Dubey, J. P.; Munson, L.; Ardans, A.

In: Parasitology, Vol. 106, No. 3, 1993, p. 239-249.

Research output: Contribution to journalArticle

Conrad, PA, Barr, BC, Sverlow, KW, Anderson, ML, Daft, B, Kinde, H, Dubey, JP, Munson, L & Ardans, A 1993, 'In vitro isolation and characterization of a Neospora sp. from aborted bovine foetuses', Parasitology, vol. 106, no. 3, pp. 239-249.
Conrad, Patricia A ; Barr, B. C. ; Sverlow, K. W. ; Anderson, Mark L ; Daft, B. ; Kinde, Hailu ; Dubey, J. P. ; Munson, L. ; Ardans, A. / In vitro isolation and characterization of a Neospora sp. from aborted bovine foetuses. In: Parasitology. 1993 ; Vol. 106, No. 3. pp. 239-249.
@article{c447f3a403fc449f94c96feda3860ab9,
title = "In vitro isolation and characterization of a Neospora sp. from aborted bovine foetuses",
abstract = "A Neospora sp. was isolated from the brains of two aborted bovine foetuses and grown continuously in vitro in bovine cell cultures. A comparison of the antigenic reactivity of in vitro cultivated tachyzoites with polyclonal antisera to Neospora caninum, Hammondia hammondi or Toxoplasma gondii revealed that the bovine protozoal isolates were similar to N. caninum and antigenically distinct from T. gondii. Tachyzoites of both bovine isolates had similar ultrastructural features, including an apical polar ring, conoid, electron-dense rhoptries and micronemes. The orientation of the micronemes, presence of micropores and a large number of electron-dense granules in the posterior portion of the bovine isolate tachyzoites differed from previous descriptions of N. caninum in vivo. Tachyzoites of the bovine isolates were ultrastructurally more similar to in vitro cultivated N. caninum tachyzoites than to tachyzoites of T. gondii or H. hammondi. The antigenic and ultrastructural similarities between N. caninum and the protozoal parasites isolated from aborted bovine foetuses in this study support the proposition that these parasites belong to the genus Neospora.",
author = "Conrad, {Patricia A} and Barr, {B. C.} and Sverlow, {K. W.} and Anderson, {Mark L} and B. Daft and Hailu Kinde and Dubey, {J. P.} and L. Munson and A. Ardans",
year = "1993",
language = "English (US)",
volume = "106",
pages = "239--249",
journal = "Parasitology",
issn = "0031-1820",
publisher = "Cambridge University Press",
number = "3",

}

TY - JOUR

T1 - In vitro isolation and characterization of a Neospora sp. from aborted bovine foetuses

AU - Conrad, Patricia A

AU - Barr, B. C.

AU - Sverlow, K. W.

AU - Anderson, Mark L

AU - Daft, B.

AU - Kinde, Hailu

AU - Dubey, J. P.

AU - Munson, L.

AU - Ardans, A.

PY - 1993

Y1 - 1993

N2 - A Neospora sp. was isolated from the brains of two aborted bovine foetuses and grown continuously in vitro in bovine cell cultures. A comparison of the antigenic reactivity of in vitro cultivated tachyzoites with polyclonal antisera to Neospora caninum, Hammondia hammondi or Toxoplasma gondii revealed that the bovine protozoal isolates were similar to N. caninum and antigenically distinct from T. gondii. Tachyzoites of both bovine isolates had similar ultrastructural features, including an apical polar ring, conoid, electron-dense rhoptries and micronemes. The orientation of the micronemes, presence of micropores and a large number of electron-dense granules in the posterior portion of the bovine isolate tachyzoites differed from previous descriptions of N. caninum in vivo. Tachyzoites of the bovine isolates were ultrastructurally more similar to in vitro cultivated N. caninum tachyzoites than to tachyzoites of T. gondii or H. hammondi. The antigenic and ultrastructural similarities between N. caninum and the protozoal parasites isolated from aborted bovine foetuses in this study support the proposition that these parasites belong to the genus Neospora.

AB - A Neospora sp. was isolated from the brains of two aborted bovine foetuses and grown continuously in vitro in bovine cell cultures. A comparison of the antigenic reactivity of in vitro cultivated tachyzoites with polyclonal antisera to Neospora caninum, Hammondia hammondi or Toxoplasma gondii revealed that the bovine protozoal isolates were similar to N. caninum and antigenically distinct from T. gondii. Tachyzoites of both bovine isolates had similar ultrastructural features, including an apical polar ring, conoid, electron-dense rhoptries and micronemes. The orientation of the micronemes, presence of micropores and a large number of electron-dense granules in the posterior portion of the bovine isolate tachyzoites differed from previous descriptions of N. caninum in vivo. Tachyzoites of the bovine isolates were ultrastructurally more similar to in vitro cultivated N. caninum tachyzoites than to tachyzoites of T. gondii or H. hammondi. The antigenic and ultrastructural similarities between N. caninum and the protozoal parasites isolated from aborted bovine foetuses in this study support the proposition that these parasites belong to the genus Neospora.

UR - http://www.scopus.com/inward/record.url?scp=0027467057&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027467057&partnerID=8YFLogxK

M3 - Article

C2 - 8488061

AN - SCOPUS:0027467057

VL - 106

SP - 239

EP - 249

JO - Parasitology

JF - Parasitology

SN - 0031-1820

IS - 3

ER -