In vitro isolation and characterisation of a bovine Neospora species in Japan

I. Yamane, T. Kokuho, K. Shimura, M. Eto, T. Shibahara, M. Haritani, Y. Ouchi, K. Sverlow, Patricia A Conrad

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Eleven aborted bovine fetuses and five calves suspected as having neosporosis were necropsied and tissues from these animals were inoculated into bovine cardiopulmonary aortic endothelial cells and monkey kidney cells and maintained at 37°C with 5 per cent CO2. Neospora tachyzoites were observed in one cell 49 days after inoculation. The isolated parasite (JPA1) was morphologically identical to the previously reported bovine Neospora species (BPA1) and confirmed by its strong antigenic reactivity with bovine control antisera to Neospora species and its lack of reactivity with Toxoplasma gondii and Sarcocystis cruzi antisera. This is the first bovine Neospora species isolate in Asia and further studies with this isolate are now expected.

Original languageEnglish (US)
Pages (from-to)77-80
Number of pages4
JournalResearch in Veterinary Science
Volume63
Issue number1
StatePublished - Jul 1997

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Neospora
Japan
cattle
antiserum
Immune Sera
Sarcocystis cruzi
Sarcocystis
Aborted Fetus
neosporosis
tachyzoites
Toxoplasma
kidney cells
Toxoplasma gondii
endothelial cells
Haplorhini
monkeys
fetus
Parasites
Endothelial Cells
In Vitro Techniques

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Yamane, I., Kokuho, T., Shimura, K., Eto, M., Shibahara, T., Haritani, M., ... Conrad, P. A. (1997). In vitro isolation and characterisation of a bovine Neospora species in Japan. Research in Veterinary Science, 63(1), 77-80.

In vitro isolation and characterisation of a bovine Neospora species in Japan. / Yamane, I.; Kokuho, T.; Shimura, K.; Eto, M.; Shibahara, T.; Haritani, M.; Ouchi, Y.; Sverlow, K.; Conrad, Patricia A.

In: Research in Veterinary Science, Vol. 63, No. 1, 07.1997, p. 77-80.

Research output: Contribution to journalArticle

Yamane, I, Kokuho, T, Shimura, K, Eto, M, Shibahara, T, Haritani, M, Ouchi, Y, Sverlow, K & Conrad, PA 1997, 'In vitro isolation and characterisation of a bovine Neospora species in Japan', Research in Veterinary Science, vol. 63, no. 1, pp. 77-80.
Yamane I, Kokuho T, Shimura K, Eto M, Shibahara T, Haritani M et al. In vitro isolation and characterisation of a bovine Neospora species in Japan. Research in Veterinary Science. 1997 Jul;63(1):77-80.
Yamane, I. ; Kokuho, T. ; Shimura, K. ; Eto, M. ; Shibahara, T. ; Haritani, M. ; Ouchi, Y. ; Sverlow, K. ; Conrad, Patricia A. / In vitro isolation and characterisation of a bovine Neospora species in Japan. In: Research in Veterinary Science. 1997 ; Vol. 63, No. 1. pp. 77-80.
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