Abstract
Eleven aborted bovine fetuses and five calves suspected as having neosporosis were necropsied and tissues from these animals were inoculated into bovine cardiopulmonary aortic endothelial cells and monkey kidney cells and maintained at 37°C with 5 per cent CO2. Neospora tachyzoites were observed in one cell 49 days after inoculation. The isolated parasite (JPA1) was morphologically identical to the previously reported bovine Neospora species (BPA1) and confirmed by its strong antigenic reactivity with bovine control antisera to Neospora species and its lack of reactivity with Toxoplasma gondii and Sarcocystis cruzi antisera. This is the first bovine Neospora species isolate in Asia and further studies with this isolate are now expected.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 77-80 |
| Number of pages | 4 |
| Journal | Research in Veterinary Science |
| Volume | 63 |
| Issue number | 1 |
| State | Published - Jul 1997 |
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ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)
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In vitro isolation and characterisation of a bovine Neospora species in Japan. / Yamane, I.; Kokuho, T.; Shimura, K.; Eto, M.; Shibahara, T.; Haritani, M.; Ouchi, Y.; Sverlow, K.; Conrad, Patricia A.
In: Research in Veterinary Science, Vol. 63, No. 1, 07.1997, p. 77-80.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro isolation and characterisation of a bovine Neospora species in Japan
AU - Yamane, I.
AU - Kokuho, T.
AU - Shimura, K.
AU - Eto, M.
AU - Shibahara, T.
AU - Haritani, M.
AU - Ouchi, Y.
AU - Sverlow, K.
AU - Conrad, Patricia A
PY - 1997/7
Y1 - 1997/7
N2 - Eleven aborted bovine fetuses and five calves suspected as having neosporosis were necropsied and tissues from these animals were inoculated into bovine cardiopulmonary aortic endothelial cells and monkey kidney cells and maintained at 37°C with 5 per cent CO2. Neospora tachyzoites were observed in one cell 49 days after inoculation. The isolated parasite (JPA1) was morphologically identical to the previously reported bovine Neospora species (BPA1) and confirmed by its strong antigenic reactivity with bovine control antisera to Neospora species and its lack of reactivity with Toxoplasma gondii and Sarcocystis cruzi antisera. This is the first bovine Neospora species isolate in Asia and further studies with this isolate are now expected.
AB - Eleven aborted bovine fetuses and five calves suspected as having neosporosis were necropsied and tissues from these animals were inoculated into bovine cardiopulmonary aortic endothelial cells and monkey kidney cells and maintained at 37°C with 5 per cent CO2. Neospora tachyzoites were observed in one cell 49 days after inoculation. The isolated parasite (JPA1) was morphologically identical to the previously reported bovine Neospora species (BPA1) and confirmed by its strong antigenic reactivity with bovine control antisera to Neospora species and its lack of reactivity with Toxoplasma gondii and Sarcocystis cruzi antisera. This is the first bovine Neospora species isolate in Asia and further studies with this isolate are now expected.
UR - http://www.scopus.com/inward/record.url?scp=0031174929&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031174929&partnerID=8YFLogxK
M3 - Article
VL - 63
SP - 77
EP - 80
JO - Research in Veterinary Science
JF - Research in Veterinary Science
SN - 0034-5288
IS - 1
ER -