In vitro isolation and characterisation of a bovine Neospora species in Japan

I. Yamane, T. Kokuho, K. Shimura, M. Eto, T. Shibahara, M. Haritani, Y. Ouchi, K. Sverlow, Patricia A Conrad

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62 Scopus citations


Eleven aborted bovine fetuses and five calves suspected as having neosporosis were necropsied and tissues from these animals were inoculated into bovine cardiopulmonary aortic endothelial cells and monkey kidney cells and maintained at 37°C with 5 per cent CO2. Neospora tachyzoites were observed in one cell 49 days after inoculation. The isolated parasite (JPA1) was morphologically identical to the previously reported bovine Neospora species (BPA1) and confirmed by its strong antigenic reactivity with bovine control antisera to Neospora species and its lack of reactivity with Toxoplasma gondii and Sarcocystis cruzi antisera. This is the first bovine Neospora species isolate in Asia and further studies with this isolate are now expected.

Original languageEnglish (US)
Pages (from-to)77-80
Number of pages4
JournalResearch in Veterinary Science
Issue number1
StatePublished - Jul 1997


ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Yamane, I., Kokuho, T., Shimura, K., Eto, M., Shibahara, T., Haritani, M., Ouchi, Y., Sverlow, K., & Conrad, P. A. (1997). In vitro isolation and characterisation of a bovine Neospora species in Japan. Research in Veterinary Science, 63(1), 77-80.