In vitro infection of primate PBMC with simian/human immunodeficiency virus, SHIV SF33A: Correlation to in vivo outcome

Janet M. Harouse, Rei Chin How Tan, Agegnehu Gettie, Peter Dailey, Preston A. Marx, Paul A Luciw, Cecilia Cheng-Mayer

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The macaque/SIV animal system is an important model for studying AIDS pathogenesis and for evaluating the efficacy of vaccines and anti-viral therapeutics. However, differences between HIV-1 and SIV envelope proteins exist that render the SIV/macaque model of limited value when examining envelope determinants of retroviral pathogenesis. To overcome this problem, we utilized a chimeric virus, SHIV SF33, containing the env gene from HIV-1 SF33 in the context of the molecular clone SIV mac239, in the macaque animal model. In this study SHIV SF33A, a pathogenic virus that evolved in vivo from a rhesus macaque infected intravenously with the molecular clone SHIV SF33 was used in both in vitro and in vivo studies. By using a cell culture system, we examined the biological properties of our parental and animal-adapted chimeric viruses and compared in vitro susceptibility to in vivo studies.

Original languageEnglish (US)
Pages (from-to)81-86
Number of pages6
JournalJournal of Medical Primatology
Volume27
Issue number2-3
StatePublished - 1998

Keywords

  • AIDS
  • Animal models
  • Pathogenesis in vitro models

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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    Harouse, J. M., Tan, R. C. H., Gettie, A., Dailey, P., Marx, P. A., Luciw, P. A., & Cheng-Mayer, C. (1998). In vitro infection of primate PBMC with simian/human immunodeficiency virus, SHIV SF33A: Correlation to in vivo outcome. Journal of Medical Primatology, 27(2-3), 81-86.