Abstract
The macaque/SIV animal system is an important model for studying AIDS pathogenesis and for evaluating the efficacy of vaccines and anti-viral therapeutics. However, differences between HIV-1 and SIV envelope proteins exist that render the SIV/macaque model of limited value when examining envelope determinants of retroviral pathogenesis. To overcome this problem, we utilized a chimeric virus, SHIV SF33, containing the env gene from HIV-1 SF33 in the context of the molecular clone SIV mac239, in the macaque animal model. In this study SHIV SF33A, a pathogenic virus that evolved in vivo from a rhesus macaque infected intravenously with the molecular clone SHIV SF33 was used in both in vitro and in vivo studies. By using a cell culture system, we examined the biological properties of our parental and animal-adapted chimeric viruses and compared in vitro susceptibility to in vivo studies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 81-86 |
| Number of pages | 6 |
| Journal | Journal of Medical Primatology |
| Volume | 27 |
| Issue number | 2-3 |
| State | Published - 1998 |
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Keywords
- AIDS
- Animal models
- Pathogenesis in vitro models
ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)
Cite this
In vitro infection of primate PBMC with simian/human immunodeficiency virus, SHIV SF33A : Correlation to in vivo outcome. / Harouse, Janet M.; Tan, Rei Chin How; Gettie, Agegnehu; Dailey, Peter; Marx, Preston A.; Luciw, Paul A; Cheng-Mayer, Cecilia.
In: Journal of Medical Primatology, Vol. 27, No. 2-3, 1998, p. 81-86.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro infection of primate PBMC with simian/human immunodeficiency virus, SHIV SF33A
T2 - Correlation to in vivo outcome
AU - Harouse, Janet M.
AU - Tan, Rei Chin How
AU - Gettie, Agegnehu
AU - Dailey, Peter
AU - Marx, Preston A.
AU - Luciw, Paul A
AU - Cheng-Mayer, Cecilia
PY - 1998
Y1 - 1998
N2 - The macaque/SIV animal system is an important model for studying AIDS pathogenesis and for evaluating the efficacy of vaccines and anti-viral therapeutics. However, differences between HIV-1 and SIV envelope proteins exist that render the SIV/macaque model of limited value when examining envelope determinants of retroviral pathogenesis. To overcome this problem, we utilized a chimeric virus, SHIV SF33, containing the env gene from HIV-1 SF33 in the context of the molecular clone SIV mac239, in the macaque animal model. In this study SHIV SF33A, a pathogenic virus that evolved in vivo from a rhesus macaque infected intravenously with the molecular clone SHIV SF33 was used in both in vitro and in vivo studies. By using a cell culture system, we examined the biological properties of our parental and animal-adapted chimeric viruses and compared in vitro susceptibility to in vivo studies.
AB - The macaque/SIV animal system is an important model for studying AIDS pathogenesis and for evaluating the efficacy of vaccines and anti-viral therapeutics. However, differences between HIV-1 and SIV envelope proteins exist that render the SIV/macaque model of limited value when examining envelope determinants of retroviral pathogenesis. To overcome this problem, we utilized a chimeric virus, SHIV SF33, containing the env gene from HIV-1 SF33 in the context of the molecular clone SIV mac239, in the macaque animal model. In this study SHIV SF33A, a pathogenic virus that evolved in vivo from a rhesus macaque infected intravenously with the molecular clone SHIV SF33 was used in both in vitro and in vivo studies. By using a cell culture system, we examined the biological properties of our parental and animal-adapted chimeric viruses and compared in vitro susceptibility to in vivo studies.
KW - AIDS
KW - Animal models
KW - Pathogenesis in vitro models
UR - http://www.scopus.com/inward/record.url?scp=0032036525&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032036525&partnerID=8YFLogxK
M3 - Article
C2 - 9747947
AN - SCOPUS:0032036525
VL - 27
SP - 81
EP - 86
JO - Journal of Medical Primatology
JF - Journal of Medical Primatology
SN - 0047-2565
IS - 2-3
ER -