In vitro infection of primate PBMC with simian/human immunodeficiency virus, SHIV SF33A: Correlation to in vivo outcome

Janet M. Harouse, Rei Chin How Tan, Agegnehu Gettie, Peter Dailey, Preston A. Marx, Paul A Luciw, Cecilia Cheng-Mayer

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The macaque/SIV animal system is an important model for studying AIDS pathogenesis and for evaluating the efficacy of vaccines and anti-viral therapeutics. However, differences between HIV-1 and SIV envelope proteins exist that render the SIV/macaque model of limited value when examining envelope determinants of retroviral pathogenesis. To overcome this problem, we utilized a chimeric virus, SHIV SF33, containing the env gene from HIV-1 SF33 in the context of the molecular clone SIV mac239, in the macaque animal model. In this study SHIV SF33A, a pathogenic virus that evolved in vivo from a rhesus macaque infected intravenously with the molecular clone SHIV SF33 was used in both in vitro and in vivo studies. By using a cell culture system, we examined the biological properties of our parental and animal-adapted chimeric viruses and compared in vitro susceptibility to in vivo studies.

Original languageEnglish (US)
Pages (from-to)81-86
Number of pages6
JournalJournal of Medical Primatology
Volume27
Issue number2-3
StatePublished - 1998

Fingerprint

Simian Immunodeficiency Virus
Human immunodeficiency virus
Macaca
Primates
HIV
in vivo studies
Human immunodeficiency virus 1
Viruses
viruses
HIV-1
pathogenesis
Clone Cells
Infection
infection
Viral Vaccines
clones
env Genes
Macaca mulatta
animals
Acquired Immunodeficiency Syndrome

Keywords

  • AIDS
  • Animal models
  • Pathogenesis in vitro models

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Harouse, J. M., Tan, R. C. H., Gettie, A., Dailey, P., Marx, P. A., Luciw, P. A., & Cheng-Mayer, C. (1998). In vitro infection of primate PBMC with simian/human immunodeficiency virus, SHIV SF33A: Correlation to in vivo outcome. Journal of Medical Primatology, 27(2-3), 81-86.

In vitro infection of primate PBMC with simian/human immunodeficiency virus, SHIV SF33A : Correlation to in vivo outcome. / Harouse, Janet M.; Tan, Rei Chin How; Gettie, Agegnehu; Dailey, Peter; Marx, Preston A.; Luciw, Paul A; Cheng-Mayer, Cecilia.

In: Journal of Medical Primatology, Vol. 27, No. 2-3, 1998, p. 81-86.

Research output: Contribution to journalArticle

Harouse, JM, Tan, RCH, Gettie, A, Dailey, P, Marx, PA, Luciw, PA & Cheng-Mayer, C 1998, 'In vitro infection of primate PBMC with simian/human immunodeficiency virus, SHIV SF33A: Correlation to in vivo outcome', Journal of Medical Primatology, vol. 27, no. 2-3, pp. 81-86.
Harouse, Janet M. ; Tan, Rei Chin How ; Gettie, Agegnehu ; Dailey, Peter ; Marx, Preston A. ; Luciw, Paul A ; Cheng-Mayer, Cecilia. / In vitro infection of primate PBMC with simian/human immunodeficiency virus, SHIV SF33A : Correlation to in vivo outcome. In: Journal of Medical Primatology. 1998 ; Vol. 27, No. 2-3. pp. 81-86.
@article{1f6e6e07afa1441e991c9c6c0c792416,
title = "In vitro infection of primate PBMC with simian/human immunodeficiency virus, SHIV SF33A: Correlation to in vivo outcome",
abstract = "The macaque/SIV animal system is an important model for studying AIDS pathogenesis and for evaluating the efficacy of vaccines and anti-viral therapeutics. However, differences between HIV-1 and SIV envelope proteins exist that render the SIV/macaque model of limited value when examining envelope determinants of retroviral pathogenesis. To overcome this problem, we utilized a chimeric virus, SHIV SF33, containing the env gene from HIV-1 SF33 in the context of the molecular clone SIV mac239, in the macaque animal model. In this study SHIV SF33A, a pathogenic virus that evolved in vivo from a rhesus macaque infected intravenously with the molecular clone SHIV SF33 was used in both in vitro and in vivo studies. By using a cell culture system, we examined the biological properties of our parental and animal-adapted chimeric viruses and compared in vitro susceptibility to in vivo studies.",
keywords = "AIDS, Animal models, Pathogenesis in vitro models",
author = "Harouse, {Janet M.} and Tan, {Rei Chin How} and Agegnehu Gettie and Peter Dailey and Marx, {Preston A.} and Luciw, {Paul A} and Cecilia Cheng-Mayer",
year = "1998",
language = "English (US)",
volume = "27",
pages = "81--86",
journal = "Journal of Medical Primatology",
issn = "0047-2565",
publisher = "Blackwell Munksgaard",
number = "2-3",

}

TY - JOUR

T1 - In vitro infection of primate PBMC with simian/human immunodeficiency virus, SHIV SF33A

T2 - Correlation to in vivo outcome

AU - Harouse, Janet M.

AU - Tan, Rei Chin How

AU - Gettie, Agegnehu

AU - Dailey, Peter

AU - Marx, Preston A.

AU - Luciw, Paul A

AU - Cheng-Mayer, Cecilia

PY - 1998

Y1 - 1998

N2 - The macaque/SIV animal system is an important model for studying AIDS pathogenesis and for evaluating the efficacy of vaccines and anti-viral therapeutics. However, differences between HIV-1 and SIV envelope proteins exist that render the SIV/macaque model of limited value when examining envelope determinants of retroviral pathogenesis. To overcome this problem, we utilized a chimeric virus, SHIV SF33, containing the env gene from HIV-1 SF33 in the context of the molecular clone SIV mac239, in the macaque animal model. In this study SHIV SF33A, a pathogenic virus that evolved in vivo from a rhesus macaque infected intravenously with the molecular clone SHIV SF33 was used in both in vitro and in vivo studies. By using a cell culture system, we examined the biological properties of our parental and animal-adapted chimeric viruses and compared in vitro susceptibility to in vivo studies.

AB - The macaque/SIV animal system is an important model for studying AIDS pathogenesis and for evaluating the efficacy of vaccines and anti-viral therapeutics. However, differences between HIV-1 and SIV envelope proteins exist that render the SIV/macaque model of limited value when examining envelope determinants of retroviral pathogenesis. To overcome this problem, we utilized a chimeric virus, SHIV SF33, containing the env gene from HIV-1 SF33 in the context of the molecular clone SIV mac239, in the macaque animal model. In this study SHIV SF33A, a pathogenic virus that evolved in vivo from a rhesus macaque infected intravenously with the molecular clone SHIV SF33 was used in both in vitro and in vivo studies. By using a cell culture system, we examined the biological properties of our parental and animal-adapted chimeric viruses and compared in vitro susceptibility to in vivo studies.

KW - AIDS

KW - Animal models

KW - Pathogenesis in vitro models

UR - http://www.scopus.com/inward/record.url?scp=0032036525&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032036525&partnerID=8YFLogxK

M3 - Article

C2 - 9747947

AN - SCOPUS:0032036525

VL - 27

SP - 81

EP - 86

JO - Journal of Medical Primatology

JF - Journal of Medical Primatology

SN - 0047-2565

IS - 2-3

ER -