In vitro HPV-11 infection of human foreskin

Lloyd H Smith, Chris Foster, Margaret E. Hitchcock, Roslyn Rivkah Isseroff

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Study of the infectious process of human papillomavirus type 11 (HPV-11) has been facilitated by the discovery that HPV11-infected neonatal human foreskin epithelium can proliferate as xenografts into condyloma-like growths within athymic nude mice. Here we describe detection of HPV-11 infection of neonatal human foreskin-derived keratinocytes, infected and cultured entirely in vitro, by use of the polymerase chain reaction and primers straddling the splice donor/ acceptor site of the most prevalent early gene HPV-11 transcript (E1^E4). Expression of the E1^E4 HPV-11 mRNA is abrogated by 60°C heat inactivation of the inoculum. HPV-11-infected foreskin explants continue to produce the E1^E4 mRNA for up to 5 weeks in culture, and second-passage keratinocytes derived from infected explant outgrowths continue to produce the E1^E4 mRNA. The in vitro system described here provides a new way to study HPV-11 infection and may be useful in evaluating early events of infection.

Original languageEnglish (US)
Pages (from-to)292-295
Number of pages4
JournalJournal of Investigative Dermatology
Volume101
Issue number3
StatePublished - Sep 1993

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Keywords

  • human papillomavirus type-11/in vitro infection/human epithelium/PCR

ASJC Scopus subject areas

  • Dermatology

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