In vitro HPV-11 infection of human foreskin

Lloyd H Smith, Chris Foster, Margaret E. Hitchcock, Roslyn Rivkah Isseroff

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Study of the infectious process of human papillomavirus type 11 (HPV-11) has been facilitated by the discovery that HPV11-infected neonatal human foreskin epithelium can proliferate as xenografts into condyloma-like growths within athymic nude mice. Here we describe detection of HPV-11 infection of neonatal human foreskin-derived keratinocytes, infected and cultured entirely in vitro, by use of the polymerase chain reaction and primers straddling the splice donor/ acceptor site of the most prevalent early gene HPV-11 transcript (E1^E4). Expression of the E1^E4 HPV-11 mRNA is abrogated by 60°C heat inactivation of the inoculum. HPV-11-infected foreskin explants continue to produce the E1^E4 mRNA for up to 5 weeks in culture, and second-passage keratinocytes derived from infected explant outgrowths continue to produce the E1^E4 mRNA. The in vitro system described here provides a new way to study HPV-11 infection and may be useful in evaluating early events of infection.

Original languageEnglish (US)
Pages (from-to)292-295
Number of pages4
JournalJournal of Investigative Dermatology
Volume101
Issue number3
StatePublished - Sep 1993

Fingerprint

Human papillomavirus 11
Foreskin
Papillomavirus Infections
Messenger RNA
RNA Splice Sites
Polymerase chain reaction
Keratinocytes
Heterografts
Nude Mice
Genes
In Vitro Techniques
Epithelium
Hot Temperature
Polymerase Chain Reaction
Growth
Infection

Keywords

  • human papillomavirus type-11/in vitro infection/human epithelium/PCR

ASJC Scopus subject areas

  • Dermatology

Cite this

In vitro HPV-11 infection of human foreskin. / Smith, Lloyd H; Foster, Chris; Hitchcock, Margaret E.; Isseroff, Roslyn Rivkah.

In: Journal of Investigative Dermatology, Vol. 101, No. 3, 09.1993, p. 292-295.

Research output: Contribution to journalArticle

Smith, Lloyd H ; Foster, Chris ; Hitchcock, Margaret E. ; Isseroff, Roslyn Rivkah. / In vitro HPV-11 infection of human foreskin. In: Journal of Investigative Dermatology. 1993 ; Vol. 101, No. 3. pp. 292-295.
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