Abstract
Study of the infectious process of human papillomavirus type 11 (HPV-11) has been facilitated by the discovery that HPV11-infected neonatal human foreskin epithelium can proliferate as xenografts into condyloma-like growths within athymic nude mice. Here we describe detection of HPV-11 infection of neonatal human foreskin-derived keratinocytes, infected and cultured entirely in vitro, by use of the polymerase chain reaction and primers straddling the splice donor/ acceptor site of the most prevalent early gene HPV-11 transcript (E1^E4). Expression of the E1^E4 HPV-11 mRNA is abrogated by 60°C heat inactivation of the inoculum. HPV-11-infected foreskin explants continue to produce the E1^E4 mRNA for up to 5 weeks in culture, and second-passage keratinocytes derived from infected explant outgrowths continue to produce the E1^E4 mRNA. The in vitro system described here provides a new way to study HPV-11 infection and may be useful in evaluating early events of infection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 292-295 |
| Number of pages | 4 |
| Journal | Journal of Investigative Dermatology |
| Volume | 101 |
| Issue number | 3 |
| State | Published - Sep 1993 |
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Keywords
- human papillomavirus type-11/in vitro infection/human epithelium/PCR
ASJC Scopus subject areas
- Dermatology
Cite this
In vitro HPV-11 infection of human foreskin. / Smith, Lloyd H; Foster, Chris; Hitchcock, Margaret E.; Isseroff, Roslyn Rivkah.
In: Journal of Investigative Dermatology, Vol. 101, No. 3, 09.1993, p. 292-295.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro HPV-11 infection of human foreskin
AU - Smith, Lloyd H
AU - Foster, Chris
AU - Hitchcock, Margaret E.
AU - Isseroff, Roslyn Rivkah
PY - 1993/9
Y1 - 1993/9
N2 - Study of the infectious process of human papillomavirus type 11 (HPV-11) has been facilitated by the discovery that HPV11-infected neonatal human foreskin epithelium can proliferate as xenografts into condyloma-like growths within athymic nude mice. Here we describe detection of HPV-11 infection of neonatal human foreskin-derived keratinocytes, infected and cultured entirely in vitro, by use of the polymerase chain reaction and primers straddling the splice donor/ acceptor site of the most prevalent early gene HPV-11 transcript (E1^E4). Expression of the E1^E4 HPV-11 mRNA is abrogated by 60°C heat inactivation of the inoculum. HPV-11-infected foreskin explants continue to produce the E1^E4 mRNA for up to 5 weeks in culture, and second-passage keratinocytes derived from infected explant outgrowths continue to produce the E1^E4 mRNA. The in vitro system described here provides a new way to study HPV-11 infection and may be useful in evaluating early events of infection.
AB - Study of the infectious process of human papillomavirus type 11 (HPV-11) has been facilitated by the discovery that HPV11-infected neonatal human foreskin epithelium can proliferate as xenografts into condyloma-like growths within athymic nude mice. Here we describe detection of HPV-11 infection of neonatal human foreskin-derived keratinocytes, infected and cultured entirely in vitro, by use of the polymerase chain reaction and primers straddling the splice donor/ acceptor site of the most prevalent early gene HPV-11 transcript (E1^E4). Expression of the E1^E4 HPV-11 mRNA is abrogated by 60°C heat inactivation of the inoculum. HPV-11-infected foreskin explants continue to produce the E1^E4 mRNA for up to 5 weeks in culture, and second-passage keratinocytes derived from infected explant outgrowths continue to produce the E1^E4 mRNA. The in vitro system described here provides a new way to study HPV-11 infection and may be useful in evaluating early events of infection.
KW - human papillomavirus type-11/in vitro infection/human epithelium/PCR
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UR - http://www.scopus.com/inward/citedby.url?scp=0027317242&partnerID=8YFLogxK
M3 - Article
C2 - 8396606
AN - SCOPUS:0027317242
VL - 101
SP - 292
EP - 295
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 3
ER -