Abstract
Parasitic plants in the Orobanchaceae invade host plant roots through root organs called haustoria. Parasite roots initiate haustorium development when exposed to specific secondary metabolites that are released into the rhizosphere by host plant roots. While molecular approaches are increasingly being taken to understand the genetic mechanism underlying these events, a limitation has been the lack of a transformation system for parasitic plants. Since the haustorium development occurs in roots of Orobanchaceae, root cultures may be suitable material for transient or stable transformation experiments. To this end, root cultures were obtained from explants, and subsequently calluses, from the hemiparasitic plant Triphysaria versicolor. The cultured roots retained their competence to form haustoria when exposed to host roots, host root exudates, or purified haustorium-inducing factors. The root culture haustoria invaded host roots and initiated a vascular continuity between the parasite and host roots. The ontogeny of haustoria development on root cultures was indistinguishable from that on seedlings roots. Root cultures should provide useful material for molecular studies of haustorium development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 257-265 |
| Number of pages | 9 |
| Journal | Plant Cell, Tissue and Organ Culture |
| Volume | 77 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 1 2004 |
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Keywords
- Haustorium development
- Orobanchaceae
- Parasitic plant
- Plant-plant interactions
- Triphysaria
ASJC Scopus subject areas
- Horticulture
Cite this
In vitro haustorium development in roots and root cultures of the hemiparasitic plant Triphysaria versicolor. / Tomilov, Alexey; Tomilova, Natalia; Yoder, John I.
In: Plant Cell, Tissue and Organ Culture, Vol. 77, No. 3, 01.06.2004, p. 257-265.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro haustorium development in roots and root cultures of the hemiparasitic plant Triphysaria versicolor
AU - Tomilov, Alexey
AU - Tomilova, Natalia
AU - Yoder, John I.
PY - 2004/6/1
Y1 - 2004/6/1
N2 - Parasitic plants in the Orobanchaceae invade host plant roots through root organs called haustoria. Parasite roots initiate haustorium development when exposed to specific secondary metabolites that are released into the rhizosphere by host plant roots. While molecular approaches are increasingly being taken to understand the genetic mechanism underlying these events, a limitation has been the lack of a transformation system for parasitic plants. Since the haustorium development occurs in roots of Orobanchaceae, root cultures may be suitable material for transient or stable transformation experiments. To this end, root cultures were obtained from explants, and subsequently calluses, from the hemiparasitic plant Triphysaria versicolor. The cultured roots retained their competence to form haustoria when exposed to host roots, host root exudates, or purified haustorium-inducing factors. The root culture haustoria invaded host roots and initiated a vascular continuity between the parasite and host roots. The ontogeny of haustoria development on root cultures was indistinguishable from that on seedlings roots. Root cultures should provide useful material for molecular studies of haustorium development.
AB - Parasitic plants in the Orobanchaceae invade host plant roots through root organs called haustoria. Parasite roots initiate haustorium development when exposed to specific secondary metabolites that are released into the rhizosphere by host plant roots. While molecular approaches are increasingly being taken to understand the genetic mechanism underlying these events, a limitation has been the lack of a transformation system for parasitic plants. Since the haustorium development occurs in roots of Orobanchaceae, root cultures may be suitable material for transient or stable transformation experiments. To this end, root cultures were obtained from explants, and subsequently calluses, from the hemiparasitic plant Triphysaria versicolor. The cultured roots retained their competence to form haustoria when exposed to host roots, host root exudates, or purified haustorium-inducing factors. The root culture haustoria invaded host roots and initiated a vascular continuity between the parasite and host roots. The ontogeny of haustoria development on root cultures was indistinguishable from that on seedlings roots. Root cultures should provide useful material for molecular studies of haustorium development.
KW - Haustorium development
KW - Orobanchaceae
KW - Parasitic plant
KW - Plant-plant interactions
KW - Triphysaria
UR - http://www.scopus.com/inward/record.url?scp=1842473189&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1842473189&partnerID=8YFLogxK
U2 - 10.1023/B:TICU.0000018392.62980.41
DO - 10.1023/B:TICU.0000018392.62980.41
M3 - Article
AN - SCOPUS:1842473189
VL - 77
SP - 257
EP - 265
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 3
ER -