In vitro functional assessment of adult spiral ganglion neurons (SGNs)

Jeong Han Lee, Choongryoul Sihn, Wanging Wang, Cristina Maria Perez Flores, Ebenezer N. Yamoah

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

Spiral ganglion neurons (SGNs) faithfully encode acoustic waves from hair cells to the cochlear nucleus (CN) using voltage-dependent ion channels. A sizable portion of our knowledge on SGN functions have been derived from pre-hearing neurons. In post-hearing SGNs, the mechanisms of how they encode the massive sound information without delay and precisely are largely unknown. Mature SGNs are housed in the central bony labyrinth of the cochlea, protected by a well-insulated myelin sheath, making it a technical feat to isolate viable neurons for rigorous functional electrophysiology. Recently, we have overcome the previous intractable hindrance in SGN functional analyses. We provide a step-by-step user-friendly protocol with practical applications, including patch-clamp recordings and imaging by using cultured SGNs.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages513-523
Number of pages11
Volume1427
DOIs
StatePublished - 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1427
ISSN (Print)10643745

Keywords

  • Auditory system
  • Hair cells (HCs)
  • Matured SGNs
  • Modiolus
  • Myelin sheath
  • Spiral ganglion neurons (SGNs)
  • Unmyelinated

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Lee, J. H., Sihn, C., Wang, W., Flores, C. M. P., & Yamoah, E. N. (2016). In vitro functional assessment of adult spiral ganglion neurons (SGNs). In Methods in Molecular Biology (Vol. 1427, pp. 513-523). (Methods in Molecular Biology; Vol. 1427). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3615-1_29