In vitro functional assessment of adult spiral ganglion neurons (SGNs)

Jeong Han Lee, Choongryoul Sihn, Wanging Wang, Cristina Maria Perez Flores, Ebenezer N. Yamoah

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Spiral ganglion neurons (SGNs) faithfully encode acoustic waves from hair cells to the cochlear nucleus (CN) using voltage-dependent ion channels. A sizable portion of our knowledge on SGN functions have been derived from pre-hearing neurons. In post-hearing SGNs, the mechanisms of how they encode the massive sound information without delay and precisely are largely unknown. Mature SGNs are housed in the central bony labyrinth of the cochlea, protected by a well-insulated myelin sheath, making it a technical feat to isolate viable neurons for rigorous functional electrophysiology. Recently, we have overcome the previous intractable hindrance in SGN functional analyses. We provide a step-by-step user-friendly protocol with practical applications, including patch-clamp recordings and imaging by using cultured SGNs.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages513-523
Number of pages11
Volume1427
DOIs
StatePublished - 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1427
ISSN (Print)10643745

Fingerprint

Spiral Ganglion
Neurons
Hearing
Cochlear Nucleus
In Vitro Techniques
Electrophysiology
Cochlea
Inner Ear
Myelin Sheath
Ion Channels

Keywords

  • Auditory system
  • Hair cells (HCs)
  • Matured SGNs
  • Modiolus
  • Myelin sheath
  • Spiral ganglion neurons (SGNs)
  • Unmyelinated

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Lee, J. H., Sihn, C., Wang, W., Flores, C. M. P., & Yamoah, E. N. (2016). In vitro functional assessment of adult spiral ganglion neurons (SGNs). In Methods in Molecular Biology (Vol. 1427, pp. 513-523). (Methods in Molecular Biology; Vol. 1427). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3615-1_29

In vitro functional assessment of adult spiral ganglion neurons (SGNs). / Lee, Jeong Han; Sihn, Choongryoul; Wang, Wanging; Flores, Cristina Maria Perez; Yamoah, Ebenezer N.

Methods in Molecular Biology. Vol. 1427 Humana Press Inc., 2016. p. 513-523 (Methods in Molecular Biology; Vol. 1427).

Research output: Chapter in Book/Report/Conference proceedingChapter

Lee, JH, Sihn, C, Wang, W, Flores, CMP & Yamoah, EN 2016, In vitro functional assessment of adult spiral ganglion neurons (SGNs). in Methods in Molecular Biology. vol. 1427, Methods in Molecular Biology, vol. 1427, Humana Press Inc., pp. 513-523. https://doi.org/10.1007/978-1-4939-3615-1_29
Lee JH, Sihn C, Wang W, Flores CMP, Yamoah EN. In vitro functional assessment of adult spiral ganglion neurons (SGNs). In Methods in Molecular Biology. Vol. 1427. Humana Press Inc. 2016. p. 513-523. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3615-1_29
Lee, Jeong Han ; Sihn, Choongryoul ; Wang, Wanging ; Flores, Cristina Maria Perez ; Yamoah, Ebenezer N. / In vitro functional assessment of adult spiral ganglion neurons (SGNs). Methods in Molecular Biology. Vol. 1427 Humana Press Inc., 2016. pp. 513-523 (Methods in Molecular Biology).
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