In vitro functional assessment of adult spiral ganglion neurons (SGNs)

Jeong Han Lee; Choongryoul Sihn; Wanging Wang; Cristina Maria Perez Flores; Ebenezer N. Yamoah

doi:10.1007/978-1-4939-3615-1_29

In vitro functional assessment of adult spiral ganglion neurons (SGNs)

Jeong Han Lee, Choongryoul Sihn, Wanging Wang, Cristina Maria Perez Flores, Ebenezer N. Yamoah

Research output: Chapter in Book/Report/Conference proceeding › Chapter

5 Scopus citations

Abstract

Spiral ganglion neurons (SGNs) faithfully encode acoustic waves from hair cells to the cochlear nucleus (CN) using voltage-dependent ion channels. A sizable portion of our knowledge on SGN functions have been derived from pre-hearing neurons. In post-hearing SGNs, the mechanisms of how they encode the massive sound information without delay and precisely are largely unknown. Mature SGNs are housed in the central bony labyrinth of the cochlea, protected by a well-insulated myelin sheath, making it a technical feat to isolate viable neurons for rigorous functional electrophysiology. Recently, we have overcome the previous intractable hindrance in SGN functional analyses. We provide a step-by-step user-friendly protocol with practical applications, including patch-clamp recordings and imaging by using cultured SGNs.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	513-523
Number of pages	11
Volume	1427
DOIs	https://doi.org/10.1007/978-1-4939-3615-1_29
State	Published - 2016
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1427
ISSN (Print)	10643745

Keywords

Auditory system
Hair cells (HCs)
Matured SGNs
Modiolus
Myelin sheath
Spiral ganglion neurons (SGNs)
Unmyelinated

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-3615-1_29

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