In vitro functional assessment of adult spiral ganglion neurons (SGNs)

Jeong Han Lee, Choongryoul Sihn, Wanging Wang, Cristina Maria Perez Flores, Ebenezer N. Yamoah

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations


Spiral ganglion neurons (SGNs) faithfully encode acoustic waves from hair cells to the cochlear nucleus (CN) using voltage-dependent ion channels. A sizable portion of our knowledge on SGN functions have been derived from pre-hearing neurons. In post-hearing SGNs, the mechanisms of how they encode the massive sound information without delay and precisely are largely unknown. Mature SGNs are housed in the central bony labyrinth of the cochlea, protected by a well-insulated myelin sheath, making it a technical feat to isolate viable neurons for rigorous functional electrophysiology. Recently, we have overcome the previous intractable hindrance in SGN functional analyses. We provide a step-by-step user-friendly protocol with practical applications, including patch-clamp recordings and imaging by using cultured SGNs.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages11
StatePublished - 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)10643745


  • Auditory system
  • Hair cells (HCs)
  • Matured SGNs
  • Modiolus
  • Myelin sheath
  • Spiral ganglion neurons (SGNs)
  • Unmyelinated

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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