Abstract
Spiral ganglion neurons (SGNs) faithfully encode acoustic waves from hair cells to the cochlear nucleus (CN) using voltage-dependent ion channels. A sizable portion of our knowledge on SGN functions have been derived from pre-hearing neurons. In post-hearing SGNs, the mechanisms of how they encode the massive sound information without delay and precisely are largely unknown. Mature SGNs are housed in the central bony labyrinth of the cochlea, protected by a well-insulated myelin sheath, making it a technical feat to isolate viable neurons for rigorous functional electrophysiology. Recently, we have overcome the previous intractable hindrance in SGN functional analyses. We provide a step-by-step user-friendly protocol with practical applications, including patch-clamp recordings and imaging by using cultured SGNs.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 513-523 |
| Number of pages | 11 |
| Volume | 1427 |
| DOIs | |
| State | Published - 2016 |
| Externally published | Yes |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1427 |
| ISSN (Print) | 10643745 |
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Keywords
- Auditory system
- Hair cells (HCs)
- Matured SGNs
- Modiolus
- Myelin sheath
- Spiral ganglion neurons (SGNs)
- Unmyelinated
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
In vitro functional assessment of adult spiral ganglion neurons (SGNs). / Lee, Jeong Han; Sihn, Choongryoul; Wang, Wanging; Flores, Cristina Maria Perez; Yamoah, Ebenezer N.
Methods in Molecular Biology. Vol. 1427 Humana Press Inc., 2016. p. 513-523 (Methods in Molecular Biology; Vol. 1427).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - In vitro functional assessment of adult spiral ganglion neurons (SGNs)
AU - Lee, Jeong Han
AU - Sihn, Choongryoul
AU - Wang, Wanging
AU - Flores, Cristina Maria Perez
AU - Yamoah, Ebenezer N.
PY - 2016
Y1 - 2016
N2 - Spiral ganglion neurons (SGNs) faithfully encode acoustic waves from hair cells to the cochlear nucleus (CN) using voltage-dependent ion channels. A sizable portion of our knowledge on SGN functions have been derived from pre-hearing neurons. In post-hearing SGNs, the mechanisms of how they encode the massive sound information without delay and precisely are largely unknown. Mature SGNs are housed in the central bony labyrinth of the cochlea, protected by a well-insulated myelin sheath, making it a technical feat to isolate viable neurons for rigorous functional electrophysiology. Recently, we have overcome the previous intractable hindrance in SGN functional analyses. We provide a step-by-step user-friendly protocol with practical applications, including patch-clamp recordings and imaging by using cultured SGNs.
AB - Spiral ganglion neurons (SGNs) faithfully encode acoustic waves from hair cells to the cochlear nucleus (CN) using voltage-dependent ion channels. A sizable portion of our knowledge on SGN functions have been derived from pre-hearing neurons. In post-hearing SGNs, the mechanisms of how they encode the massive sound information without delay and precisely are largely unknown. Mature SGNs are housed in the central bony labyrinth of the cochlea, protected by a well-insulated myelin sheath, making it a technical feat to isolate viable neurons for rigorous functional electrophysiology. Recently, we have overcome the previous intractable hindrance in SGN functional analyses. We provide a step-by-step user-friendly protocol with practical applications, including patch-clamp recordings and imaging by using cultured SGNs.
KW - Auditory system
KW - Hair cells (HCs)
KW - Matured SGNs
KW - Modiolus
KW - Myelin sheath
KW - Spiral ganglion neurons (SGNs)
KW - Unmyelinated
UR - http://www.scopus.com/inward/record.url?scp=84990173567&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84990173567&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-3615-1_29
DO - 10.1007/978-1-4939-3615-1_29
M3 - Chapter
AN - SCOPUS:84990173567
VL - 1427
T3 - Methods in Molecular Biology
SP - 513
EP - 523
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -