@inbook{7b2368b7940c4eba809be36af1c0f09e,
title = "In vitro functional assessment of adult spiral ganglion neurons (SGNs)",
abstract = "Spiral ganglion neurons (SGNs) faithfully encode acoustic waves from hair cells to the cochlear nucleus (CN) using voltage-dependent ion channels. A sizable portion of our knowledge on SGN functions have been derived from pre-hearing neurons. In post-hearing SGNs, the mechanisms of how they encode the massive sound information without delay and precisely are largely unknown. Mature SGNs are housed in the central bony labyrinth of the cochlea, protected by a well-insulated myelin sheath, making it a technical feat to isolate viable neurons for rigorous functional electrophysiology. Recently, we have overcome the previous intractable hindrance in SGN functional analyses. We provide a step-by-step user-friendly protocol with practical applications, including patch-clamp recordings and imaging by using cultured SGNs.",
keywords = "Auditory system, Hair cells (HCs), Matured SGNs, Modiolus, Myelin sheath, Spiral ganglion neurons (SGNs), Unmyelinated",
author = "Lee, {Jeong Han} and Choongryoul Sihn and Wanging Wang and Flores, {Cristina Maria Perez} and Yamoah, {Ebenezer N.}",
year = "2016",
doi = "10.1007/978-1-4939-3615-1_29",
language = "English (US)",
volume = "1427",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "513--523",
booktitle = "Methods in Molecular Biology",
}