In vitro fermentability of human milk oligosaccharides by several strains of bifidobacteria

Robert E. Ward, Milady Niñonuevo, David A. Mills, Carlito B Lebrilla, J. Bruce German

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169 Scopus citations


This study was conducted to investigate the catabolism and fermentation of human milk oligosaccharides (HMO) by individual strains of bifidobacteria. Oligosaccharides were isolated from a pooled sample of human milk using solid-phase extraction, and then added to a growth medium as the sole source of fermentable carbohydrate. Of five strains of bifidobacteria tested (Bifidobacterium longum biovar infantis, Bifidobacterium bifidum, Bifidobacterium longum biovar longum, Bifidobacterium breve, and Bifidobacterium adolescentis), B. longum bv. infantis grew better, achieving triple the cell density then the other strains. 73. bifidum did not reach a high cell density, yet generated free sialic acid, fucose and N-acetylglucosamine in the media, suggesting some capacity for HMO degradation. Thin layer chromatography profiles of spent fermentation broth suggests substantial degradation of oligosaccharides by 73. longum bv. infantis, moderate degradation by B. bifidum and little degradation by other strains. While all strains were able to individually ferment two monosaccharide constituents of HMO, glucose and galactose, only 73. longum bv. infantis and B. breve were able to ferment glucosamine, fucose and sialic acid. These results suggest that as a potential prebiotic, HMO may selectively promote the growth of certain bifidobacteria strains, and their catabolism may result in free monosaccharides in the colonic lumen.

Original languageEnglish (US)
Pages (from-to)1398-1405
Number of pages8
JournalMolecular Nutrition and Food Research
Issue number11
StatePublished - Nov 2007


  • Bifidobacteria
  • Fermentation
  • Milk
  • Oligosaccharide
  • Prebiotic

ASJC Scopus subject areas

  • Food Science


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