In vitro exposure to environmental tobacco smoke induces CYP1B1 expression in human luteinized granulosa cells

Justin D. Vidal, Catherine A. VandeVoort, Craig B. Marcus, Nicolas R. Lazarewicz, Alan J Conley

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Women smokers and women exposed to environmental tobacco smoke (ETS) have reduced ovarian function as evidenced by an earlier menopause, reduced follicular numbers, decreased levels of circulating estradiol, and decreased conception rates; however, the mechanism of action of altered ovarian function by ETS is poorly understood. The direct effects of ETS were evaluated using human luteinized granulosa cells (HLGCs) exposed to ETS in primary cell culture. Exposure to ETS caused a decrease in both estradiol and progesterone production. There was a concentration dependent increase in CYP1B1 gene and protein expression without a change in catechol-O-methyltransferase (COMT) expression. This is the first report of CYP1B1 induction secondary to ETS exposure in cells from the human ovary. CYP1B1 metabolizes both endogenous estrogens and polyaromatic hydrocarbons in ETS to a variety of reactive species and may contribute to the complex effects of ETS on ovarian function.

Original languageEnglish (US)
Pages (from-to)731-737
Number of pages7
JournalReproductive Toxicology
Volume22
Issue number4
DOIs
StatePublished - Nov 2006

Keywords

  • Catechol-O-methyltransferase
  • Corpus luteum
  • CYP1B1
  • Environmental tobacco smoke
  • Estradiol
  • Ovary
  • Progesterone

ASJC Scopus subject areas

  • Toxicology

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